Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity

Kumar, Rajesh; Mishra, Ajay Kumar; Dubey, Nawal Kishore; Tripathi, Yamini B.

doi:10.1016/j.ijfoodmicro.2006.10.017

Cited by 268 publications

(188 citation statements)

References 26 publications

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“…However, the oil of the same plant evaluated with the poisoned food technique completely inhibited mycelial growth of this filamentous fungi at 100 µg/mL (Kumar et al, 2007). This difference in antifungal activity between essential oils from plants of the same species can be explained by the chemotype (chemical composition), geographical region of plant collection, age of plant, extraction method of oils, method used to assess the antifungal activity or by the intra or inter species differences (Samaranayake et al, 1995;Cruz et al, 2007;Maksimovic et al, 2008).…”

Section: Resultsmentioning

confidence: 95%

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Correa-Royero¹,

Tangarife²,

Durán³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Atividade antifúngica in vitro e os efeitos citotóxicos de óleos essenciais e extratos de plantas medicinais e aromáticas contra Candida krusei e Aspergillus fumigatus" As plantas são geralmente utilizadas na medicina tradicional como agentes antimicrobianos e seus óleos essenciais e extratos foram conhecidos por possuir atividade antifúngica. O objetivo deste estudo foi avaliar in vitro a atividade de 32 óleos essenciais e 29 extratos contra Candida krusei e Aspergillus fumigatus, bem como o efeito citotóxico em células Vero. A curva do tempo-morte e a interação entre antifúngicos e Chenopodium ambrosioidese do extrato de Myrcia cucullata mostraram atividade antifúngica contra C. krusei (geometric means of the minimal inhibitory concentration [GM-MIC] 7,82 e 31,25 µg/ mL, respectivamente). Lippia citriodora foi ativa contra C. krusei e A. fumigatus (GM-CIM = 99,21 µg/mL e 62,5 µg/mL, respectivamente). Os testes de tempo-morte feitos com óleo de C. ambrosioides mostraram atividade fungicida em 4x MIC. A interação do óleo C. ambrosioides com itraconazol e anfotericina B foi testada pela técnica de xadrez. Nenhuma interação foi detectada pela combinação do óleo C. ambrosioides com anfotericina B e itraconazol (intervalo fractional inhibitory index [FICI] = 1,03-1,06 e 1,03-1,00, respectivamente). Os ensaios de citotoxicidade para todas as amostras foram realizadas com MTT. Apenas os óleos Hedyosmun sp. e L. dulcis foram citotóxicos.Unitermos: Candida krusei, Aspergillus fumigatus, óleos essenciais, extratos, curva do tempomorte, técnica de xadrez. ABSTRACT:The plants are usually used in traditional medicine as antimicrobial agents and their essential oils and extracts have been known to possess antifungal activity. The aim of this study was to evaluate the activity of 32 essential oils and 29 extracts in vitro against C. krusei and A. fumigatus as well as the cytotoxic effect on Vero cells. Time-kill curve and interaction between antifungal and the most active sample against C. krusei, was also evaluated. The oils from C. ambrosioides and the extract of M. cucullata showed antifungal activity against C. krusei (GM-MIC 7.82 and 31.25 µg/mL, respectively). L. citriodora was active against C. krusei and A. fumigates (GM-MIC = 99.21 µg/mL and 62.5 µg/mL respectively). Time-kill assays done with C. ambrosioides oil showed fungicidal activity at 4x MIC. The interaction of C. ambrosioides oil with itraconazole and amphotericin B was tested following the chequerboard technique. No interaction was detected for the combination of C. ambrosioides oil with amphotericin B and itraconazole (FICI range = 1.03-1.06 and 1.03-1.00, respectively). Cytotoxicity assays for all samples were carried out with MTT. Only the oil from Hedyosmun sp. and L. dulcis were cytotoxic.

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Section: Resultsmentioning

confidence: 95%

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Correa-Royero¹,

Tangarife²,

Durán³

et al. 2010

Rev. bras. farmacogn.

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“…However, resistance to chemotherapy is the primary problem in giardiasis treatment. Therefore, there is a pressing need for a low-cost drug with rapid and efficacious antigiardial action that has no toxicity or side effects (Kumar et al 2007). FC appears to be an effective and safe alternative for the treatment of resistant infections.…”

Section: Discussionmentioning

confidence: 99%

The anti-giardial effectiveness of fungal and commercial chitosan against Giardia intestinalis cysts in vitro

et al. 2014

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Chitosan with poly-N-acetylglucosamine sequences is a deacetylated derivative of chitin that can be found in the exoskeletons of crabs, shrimp and lobsters, the cuticles of insects and the cell walls of fungi. The aim of the present study was to compare the effects of fungal chitosan (FC) prepared from the cell walls of Penicillium viridicatum and Penicillium aurantiogriseum with commercially available chitosan (CC) against Giardia intestinalis cysts in vitro. The giardia cysts were isolated using a sucrose method. Four concentrations (50, 100, 200 and 400 lg/ml) of each type of prepared chitosan were applied for 10, 30, 60 and 180 min. The viability of the cysts was checked via 0.1 % eosin staining. Our results indicate that P. viridicatum (with a 47.5 % DD) and P. aurantiogriseum (with a 47.3 % DD) at different concentrations after 180 min precipitated, respectively, 56, 69, 81 and 100 %, and 63, 75, 86 and 100 % mortality rates. CC (with a 54 % DD) showed 79, 84, 93 and 100 % mortality rates. In conclusion, both FC and CC at 400 lg/ml concentrations after 180 min of exposure showed the most potent effect against G. intestinalis cysts. Accordingly, chitosan could be suggested as a new natural nanoform agent for future research in the safe and effective treatment of Giardia infections.

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“…The antioxidant activity by ABTS radical cation scavenging was determined according to methodology previously described (Kumar, Mishra, Dubey, & Tripathi, 2007;Re et al, 1999). ABTS radical was freshly prepared by adding 5 mL of 2.45 mM potassium persulphate solution to 5 mL of a 7 mM ABTS solution and kept for 12 h in dark.…”

Section: Abts Radical Cation Scavenging Activitymentioning

confidence: 99%

Phenolic content and antioxidant capacity of extracts ofLaurus nobilisL.,Coriandrum sativumL. andAmaranthus hybridusL.

Muñiz‐Márquez

Rodríguez

Balagurusamy

et al. 2013

CyTA - Journal of Food

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Extraction of polyphenols of Laurus nobilis L. (Lauraceae), Coriandrum sativum L. (Apiaceae) and Amaranthus hybridus L. (Amaranthaceae) was carried out by solid-liquid extraction. The effect of ethanol concentration and the extraction time were determined to obtain the maximum of polyphenols. Three different methods, DPPH radical scavenging, ABTS radical cation scavenging and lipid oxidation inhibition, were tested to determine the antioxidant capacity in the extracts. The results indicate that L. nobilis has a high antioxidant potential: 94.73%, 47.71% and 76.86% for DPPH, ABTS and lipid oxidation inhibition, respectively. On the other hand, C. sativum and A. hybridus showed 13.69% and 10.16% in DPPH assay, and 9.22% and 14.96% in ABTS assay, while it did not show any antioxidant capacity in the lipid oxidation inhibition method under the experimental conditions. In addition, the phenolic compounds in the extracts were also characterized by High Performance Liquid Chromatography (HPLC) analysis. (Amaranthaceae) se llevó a cabo en una extracción sólido-líquido. El efecto de la concentración de etanol y el tiempo de extracción fueron evaluados para obtener el máximo de polifenoles. Tres diferentes métodos incluyendo el radical de barrido DPPH, radical catión de barrido ABTS y la inhibición de la oxidación lipídica fueron probados para determinar la capacidad antioxidante en los extractos. Los resultados indican que L. nobilis presentó mayor potencial antioxidante con un 94,73, 47,71 y 76,86% para DPPH, ABTS e inhibición de la oxidación lipídica respectivamente. Por otra parte, C. sativum y A. hybridus mostraron un 13,69 and 10,16% en el ensayo de DPPH, 9,22 y 14,96% en el ensayo de ABTS mientras que para la inhibición de la oxidación lipídica no tuvieron capacidad antioxidante bajo las condiciones probadas. Además, la caracterización de compuestos fenólicos en los extractos también fue estudiada por el análisis de Cromatografía Líquida de Alta Resolución (HPLC).

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Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity

Cited by 268 publications

References 26 publications

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

The anti-giardial effectiveness of fungal and commercial chitosan against Giardia intestinalis cysts in vitro

Phenolic content and antioxidant capacity of extracts ofLaurus nobilisL.,Coriandrum sativumL. andAmaranthus hybridusL.

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