Evaluation of Chinese medicinal herbs fingerprinting by HPLC‐DAD for the detection of toxic aristolochic acids

Abstract

Aristolochic acids are known to contribute to various renal disorders; therefore, expanding the availability of analytical methodology to detect these compounds is important in order to assess the quality of Chinese herbal medicines in which they can be found. Twelve medicinal herbal samples were procured from various sources and extracted in duplicate prior to a "fingerprint" analysis using conventional HPLC-DAD. Multivariate analysis was performed on the entire chromatographed fingerprints. The resulting output was a partial least-square discriminant analysis model, which was able to evaluate the potential presence of aristolochic acids I and II as well as providing an individual herbal "fingerprint". The results of this study provide evidence that the presence of aristolochic acids contained within certain herbal extractions could be detected using a simple method, although some limitations apply to this method for quality control, since newly detected samples for aristolochic acid (positives) will need further confirmation with purity checks or MS hyphenation.

Aristolochic Acids in the Roots of Aristolochia Chilensis, a Dangerous Chilean Medicinal Plant

“…[14][15][16][17][18][19][20][21][22][23][24] Their UV spectra ( Figure Nº 1) and retention times were compared, and co-injection was performed using standards of AA-I (1), AA-II (2), AA-III (3), AA-IV (4), AA-Ia (5), AA-IIIa (6), and AA-IVa (7), which had been previously isolated from A. chilensis, A. argentina, and Battus polydamas archidamas 22,25,26 . Quantification was based on peak areas of chromatograms taken at 254 nm.…”

“…In this study, high-performance liquid chromatography with diodearray detection (HPLC-DAD), a technique widely used for the detection and quantification of AAs in herbal medicine [14][15][16][17][18][19][20][21][22][23][24] was used to determine the chemical composition of AAs from the roots of A. chilensis. The findings reveal that the roots contain a significant amount of AAs, making it dangerous to use A. chilensis as a medicinal plant because of the harmful effects of AAs.…”

Quantitative and qualitative determination of LiuweiDihuang preparations by ultra high performance liquid chromatography in dual‐wavelength fingerprinting mode and random forest

“…Previous studies on QC methods for traditional Chinese formulas have predominantly used only single wavelength detection to detect and/or quantify only a few compounds acting as biomarkers in the sample. Methods incorporating dual‐wavelength and multi‐wavelength detection could be more suitable for the QC of traditional Chinese formulas . In our study, two wavelengths, 210 and 238 nm, were used for detection.…”

Selective analysis of aristolochic acid I in herbal medicines by dummy molecularly imprinted solid‐phase extraction and HPLC

“…Due to the nephrotoxic actions, some manufacturers usually apply alkali to mask the AAs in order not to be detected. Generally, classical chromatography methods like HPLC with different detectors , TLC , CE were applied for analysis of AAI in complex mixtures and traditional Chinese medicine (TCMs). Owing to the trace levels of AAI and complexity of actual TCM samples, the sample preparation process has become a key factor and procedure for instrumental analysis.…”