Evaluation of CHROMagar™-Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens

Pérez-Viso, Blanca; Aracil‐Gisbert, Sonia; Coque, Teresa M.; Campo, Rosa del; Ruíz-Garbajosa, Patricia

doi:10.1007/s10096-021-04328-w

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…The higher speed in diagnostics may be the major advantage of the PCR technique. In comparison to the microbial culture, Neonatology 2023;120:176-184 DOI: 10.1159/000526836 the same-day result of the PCR diagnostics is extremely valuable for cohorting and barrier nursing as recommended in the German guidelines for infection prevention and control in neonates [12]; even if a quicker approach using Serratia-selective agar plates has recently become available [23]. Comparing the costs (consumables + personnel) for the initial culture-based strain identification with antibiotic resistance testing versus that for PCR-based screening in the entire cohort of 505 patients enrolled in this study, the novel method was about 3-fold more expensive than the routine culture.…”

Section: Discussionmentioning

confidence: 99%

Validation of a New PCR-Based Screening Method for Prevention of Serratia marcescens Outbreaks in the Neonatal Intensive Care Unit

et al. 2023

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Background: Serratia marcescens may cause severe nosocomial infections, mostly in very low birth weight infants. Since S. marcescens exhibits by far the highest adjusted incidence rate for horizontal transmission, it can cause complex outbreak situations in neonatal intensive care units. Objective: The aim of this study was to establish a fast and highly sensitive colonization screening for prompt cohorting and barrier nursing strategies. Methods: A probe-based duplex PCR assay targeting the 16S rRNA gene of S. marcescens was developed and validated by using 36 reference strains, 14 S. marcescens outbreak- and nonoutbreak isolates, defined by epidemiological linkage and molecular typing, and applied in 1,347 clinical specimens from 505 patients. Results and Conclusions: The novel PCR assay proved to be highly specific and had an in vitro sensitivity of 100 gene copies per reaction (∼15 bacteria). It showed a similar (in laryngeal/tracheal specimens) or even higher (in rectal/stoma swabs) in vivo sensitivity in comparison to routine microbial culture and was much quicker (<24 h vs. 2 days). By combining different oligonucleotide primers, there was robust detection of genetic variants of S. marcescens strains. PCR inhibition was low (1.6%) and observed with rectal swabs only. Cohort analysis illustrated applicability of the PCR assay as a quick tool to prevent outbreak scenarios by allowing rapid decisions on cohorting and barrier nursing. In summary, this novel molecular screening for colonization by S. marcescens is specific, highly sensitive, and substantially accelerates detection.

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Section: Discussionmentioning

confidence: 99%

Validation of a New PCR-Based Screening Method for Prevention of Serratia marcescens Outbreaks in the Neonatal Intensive Care Unit

et al. 2023

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Yersiniaceae

Kingry,

Gladney,

Petersen

2023

ClinMicroNow

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The family Yersiniaceae includes two well‐known pathogenic genera, Serratia and Yersinia , and multiple other genera which inhabit a wide range of ecological niches. Serratia marcescens is the Serratia species most often isolated from clinical specimens and can cause urinary tract infections, pneumonia, bloodstream infections, endocarditis, and wound infections. This chapter primarily focuses on the taxonomy, detection, and identification of Serratia and Yersinia . Yersiniaceae members are Gram‐negative rods that are non‐spore‐forming, oxidase‐negative, catalase‐positive facultative anaerobes which display nonfastidious growth. The Yersiniaceae are widely distributed throughout the environment and transmitted to humans via arthropod bites, contact with infected animals, ingestion of contaminated food or water, or exposure to hospital environments and medical procedures. Serology can be used as an adjunct in the diagnosis of disease due to Yersinia enterocolitica or Yersinia pseudotuberculosis . Many S. marcescens strains carry both chromosomally encoded and plasmid‐mediated resistance determinants for several different types of antimicrobials.

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Evaluation of CHROMagar™ B. cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis

Maruri-Aransolo,

de Dios Caballero,

Michelena

et al. 2024

Eur J Clin Microbiol Infect Dis

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Introduction Burkholderia cepacia complex (BCC) are non-fermenting Gram-negative bacteria that can chronically colonize the lungs of people with cystic fibrosis (pwCF), causing a severe and progressive respiratory failure, post-transplant complications and epidemic outbreaks. Therefore, rapid and accurate identification of these bacteria is relevant for pwCF, in order to facilitate early eradication and prevent chronic colonization. However, BCCs are often quite difficult to detect on culture media as they have a slow growth rate and can be hidden by other fast-growing microorganisms, including Pseudomonas aeruginosa and filamentous fungi. Material and methods We evaluated the sensitivity of CHROMagar™ B. cepacia agar using 11 isolates from a well-characterized BCC collection, using BCA agar (Oxoid, UK) as a gold standard. We also studied 180 clinical sputum samples to calculate positive (PPV) and negative (NPV) predictive values. Furthermore, we used three of the well-characterized BCC isolates to determine the limit of detection (LOD). Results Eleven isolates grew on CHROMagar™ B. cepacia at 37ºC after 48 h. The NPV and PPV of CHROMagar™ B. cepacia were 100% and 87.5%, respectively. The LOD of CHROMagar™ B. cepacia was around 1 × 103 CFU/ml, requiring a ten-fold dilution lower bacterial load than BCA for BCC detection. Conclusion CHROMagar™ B. cepacia agar proved to have a very good sensitivity and specificity for the detection of clinical BCCs. Moreover, the chromogenic nature of the medium allowed us to clearly differentiate BCC from other Gram-negative species, filamentous fungi and yeasts, thereby facilitating the identification of contaminants.

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Evaluation of CHROMagar™-Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens

Cited by 3 publications

References 9 publications

Validation of a New PCR-Based Screening Method for Prevention of Serratia marcescens Outbreaks in the Neonatal Intensive Care Unit

Validation of a New PCR-Based Screening Method for Prevention of Serratia marcescens Outbreaks in the Neonatal Intensive Care Unit

Yersiniaceae

Evaluation of CHROMagar™ B. cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis

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