Evaluation of circulating endothelial cells in the rat after acute and fractionated whole-body gamma irradiation

Al-Massarani, Ghassan; Almohamad, Khaled

doi:10.2478/nuka-2014-0021

Cited by 3 publications

(3 citation statements)

References 49 publications

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“…These cells are heterogeneous in size (15–50 µm), carry the markers of ECs (e.g., VWF, CD144 and CD146), but not leukocyte markers (CD45), and serve as noninvasive markers of EC damage and dysfunction. Al-Massarani and colleagues demonstrated a reduction in circulating ECs after acute- and fractionated-dose gamma irradiation in rat peripheral blood, with an incomplete recovery 2 months postirradiaton (91), and in patients undergoing chemotherapy (92, 93). The presence of systemic signalers of processes, such as the oxidative stress pathway and inflammation, is attractive since there is vast potential for a noninvasive or minimally invasive source of biomarkers for early and delayed progression of injury.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

2016

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After the events of September 11, 2001, a decade of research on the development of medical countermeasures (MCMs) to treat victims of a radiological incident has yielded two FDA-approved agents to mitigate acute radiation syndrome. These licensed agents specifically target the mitigation of radiation-induced neutropenia and infection potential, while the ramifications of the exposure event in a public health emergency incident could include the entire body, causing additional acute and/or delayed organ/tissue injuries. Anecdotal data as well as recent findings from both radiation accident survivors and animal experiments implicate radiation-induced injury or dysfunction of the vascular endothelium leading to tissue and organ injuries. There are significant gaps in our understanding of the disease processes and progression, as well as the optimum approaches to develop medical countermeasures to mitigate radiation vascular injury. To address this issue, the Radiation and Nuclear Countermeasures Program of the National Institute of Allergy and Infectious Diseases (NIAID) organized a one-day workshop to examine the current state of the science in radiation-induced vascular injuries and organ dysfunction, the natural history of the pathophysiology and the product development maturity of potential medical countermeasures to treat these injuries. Meeting presentations were followed by a NIAID-led open discussion among academic investigators, industry researchers and government agency representatives. This article provides a summary of these presentations and subsequent discussion from the workshop.

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Section: Introductionmentioning

confidence: 99%

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

2016

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“…In a previous study, our group reported a gradual decline in CEC count post irradiation (AL-Massarani and Almohamad, 2014).…”

Section: A B D Cmentioning

confidence: 63%

“…In a previous study, our group reported a gradual decline in CEC count post irradiation ( AL-Massarani and Almohamad, 2014 ). Originally, the results of this study ( Figure 6 ) suggest that injection of RSV attenuates radiation-induced CEC apoptosis in rats.…”

Section: Discussionmentioning

confidence: 77%

Resveratrol Modulates the Inflammatory Profile of Immune Responses and Circulating Endothelial Cells’ (CECs’) Population During Acute Whole Body Gamma Irradiation

et al. 2020

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Wistar rats were whole body irradiated with a single dose of 2 Gy post administration with 10 or 100 mg/kg of resveratrol (RSV) intraperitoneally for 30 days. Rats’ livers were dissected and processed to analyze immune response profiles of Th1, Th2, Th9, Th17, and Th22 by flow cytometry. In addition, peripheral blood samples were collected and circulating endothelial cells (CECs) were counted as an indicator for endothelial damage. Results demonstrated that resveratrol at 100 mg/kg enhanced liver immunological response influenced by irradiation by inducing Th2 immune response that was revealed by an increase in IL-10 secretion to more than 5,000 pmol/ml post irradiation. Results also indicated that RSV, at a dose of 100 mg/kg, decreased levels of the main pro-inflammatory cytokines such as INF- γ , IL-22, IL-17A, and GM-CSF post irradiation. In addition, the same RSV was bound to upregulate the expression of IL-10 mRNA in isolated Kupffer cells (KCs) and their secretion of IL-10 post irradiation. The result demonstrated that KCs were the central source of this anti-inflammatory response mediated mainly by IL10. These results, proposed for the first time, clearly states that RSV promotes IL-10 mediated immune resolution by Kupffer cells and not by hepatocytes. This implies that KCs have a crucial role in radiotherapy. Additionally, this study showed that RSV had an anti-apoptotic effect through re-increasing the number of CECs, which is implicated in irradiation damage. Result of the current work discloses novel findings about the potential of RSV as a radio-protector agent of a natural origin and suggests novel roles of KCs as a pharmacological target during radiation exposure.

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Human radiation exposures (occupational, medical, environmental, and radiation incidents) and vascular dysfunction

DiCarlo

Satyamitra

2021

Endothelial Signaling in Vascular Dysfunction and Disease

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Evaluation of circulating endothelial cells in the rat after acute and fractionated whole-body gamma irradiation

Cited by 3 publications

References 49 publications

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

Resveratrol Modulates the Inflammatory Profile of Immune Responses and Circulating Endothelial Cells’ (CECs’) Population During Acute Whole Body Gamma Irradiation

Human radiation exposures (occupational, medical, environmental, and radiation incidents) and vascular dysfunction

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