Evaluation of Circulating MicroRNA-221 and Il-10 Levels as Potential Diagnostic Markers for Colorectal Cancer in Correlation with Prognosis

Abstract

Background: Because of the capacity of MicroRNAs to down-regulate tumor suppressor expression and enhance tumorigenesis, they are of great importance in the knowledge of cancer. Aim of the study: To study the performance of miR-221 and IL-10 in colorectal carcinoma. Patients and methods: In the present study, venous blood specimens were taken from (55) patient suffering from colorectal cancer who were attending the Medical City, Baghdad (Teaching laboratories), during the period from 1st Jun. 2018 to 30th Sep. 2019. For detection of miRNA-221 expression in patient’s sera, the Real-time polymerase chain reaction (PCR) was used. Results: Descriptive analysis of miRNA-221 expression according to pathological grading and gender showed no significant difference (p > 0.05), however, variations of miRNA-221 expression among patient and controls revealed significant differences (p < 0.05). There was a highly significant difference in the mean serum IL-10 levels among patients with CRC when compared with the control group (p > 0.04). While the highest disease risk was correlated to higher mi-RNA-221 with IL-10 levels related with poor prognosis. Conclusion: The high expressions of mi-RNA-221 can act as prognostic markers in patients with colorectal cancer. Besides, the higher levels of miRNA-221 and IL-10 were correlated with worse prognosis.