Evaluation of cisplatin + 5-FU, carboplatin + 5-FU, and ifosfamide + epirubicine regimens using the micronuclei test and nuclear abnormalities in the buccal mucosa

Torres-Bugarı́n, Olivia; Ventura-Aguilar, A.J.; Zamora‐Pérez, Ana Lourdes; Gómez‐Meda, Belinda Claudia; Ramos-Ibarra, María Luisa; Morgan-Villela, Gilberto; Gutiérrez-Franco, A.; Zúñiga‐González, Guillermo Moisés

doi:10.1016/s1383-5718(03)00163-3

Cited by 54 publications

(30 citation statements)

References 23 publications

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“…In Chinese patients with nasopharyngeal cancer 2.3 ‰ cells with MN were observed [26] which is very close to the data reported concerning the healthy persons from China [36]. As for Mexican cancer patients, in all three presented groups the number of cells with MN significantly increased compared with 43 healthy persons from the same study (0.6 ‰) [18]. But at the same time 37 primary cancer patients with various localization of tumors in the same study used as a control did not show any increase compared with healthy subjects (0.8 ‰).…”

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levesupporting

confidence: 85%

“…Significantly increased number of MN was observed. In 21 Mexican patients with various locali zation of tumors treated with isophospha mide+epirubicin the significantly increased number of MN was registered (from 1.2 ‰ before to 2.6 ‰ after treatment) [18]. In 14 patients (mostly with oral and penis cancer) treated with carboplatin + 5 fluo rouracil and 6 patients (3 with penis, 1 with prostate, and 2 with oral cancers) treated with cisDDP+5 fluorouracil no such effect was registered.…”

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levementioning

confidence: 94%

“…In all cases not significant increase of MN was observed. Torres Bugarin et al [18] mentioned that many patients under chemo therapy had signs of toxicity -the presence of a lot of buccal cell with karyolysis. Hence, 2 papers re ported about significant increment of MN induced by antiblastic chemotherapy and 1 the same effect only due to one schedule of therapy.…”

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levementioning

confidence: 99%

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Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Nersesyan

2007

Cytol. Genet.

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Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levesupporting

confidence: 85%

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levementioning

confidence: 94%

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levementioning

confidence: 99%

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Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Nersesyan

2007

Cytol. Genet.

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“…Este resultado sugere que esta estrutura não está relacionada à genotoxicidade. A ausência de associação positiva entre broken eggs e insulto genotóxico também foi relatada por diversos autores (BOHRER et al, 2005;CERQUEIRA et al, 2008;HOLLAND et al, 2008;TORRES-BUGARÍN et al, 2003). Segundo Holland et al (2008) broken eggs está relacionado com amplificação gênica e não representa danos ao DNA.…”

Section: Discussionunclassified

Danos cromossômicos e alterações nucleares em células esfoliadas do epitélio gengival de indivíduos com periodontite crônica moderada

Silva

Neris²,

Lopes

et al. 2017

cmbio

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ResumoIntrodução: a periodontite é uma doença multifatorial que resulta primariamente da ação de bactérias nos tecidos que compõem o periodonto. O processo inflamatório que se desenvolve em consequência está associado ao aumento da proliferação celular que propicia a ocorrência de danos no material genético. Objetivo: avaliar, com uso do teste de micronúcleo, a ocorrência de danos genéticos e alterações nucleares em indivíduos com periodontite crônica moderada. Metodologia: a amostra incluiu dez indivíduos portadores de periodontite crônica moderada em início do tratamento no Centro de Especialidades Odontológicas (CEO) de Senhor do Bonfim/BA. Os participantes responderam a um questionário e células esfoliadas da mucosa gengival de regiões sem alterações e com periodontite crônica moderada foram coletadas. Este material foi transferido para lâmina de vidro, fixado com metanol/ácido acético (3:1), corado com reativo de Shiff e fast green e analisado sob microscopia óptica. A análise estatística foi feita com o teste condicional para comparação de proporções em situações de eventos raros. Resultados: a ocorrência de micronúcleos, alterações nucleares degenerativas (cariorréxis, cariólise, cromatina condensada, picnose) e vacúolos nucleares foi significativamente maior em células da lesão. A ocorrência de broken eggs não diferiu estatísticamente. Conclusões: a periodontite crônica moderada é uma doença na qual há comprometimento da integridade genética das células do epitélio gengival. A maior ocorrência de alterações indicativas de apoptose na área da periodontite crônica aponta para a resposta biológica do organismo ao insulto genotóxico ao qual as células nesta lesão estão submetidas. Palavras-chave: Periodontite. Genotoxicidade. Apoptose. Abstract Introduction: periodontitis is a multifactorial disease that results primarily from the action of bacteria in the tissues that compose the periodontium. The inflammatory process that develops as a consequence is associated with increased cell proliferation which facilitates

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“…One cytological abnormality that has been studied as an indicator of pathological alterations in certain diseases is the appearance of cells with nuclear buds (BE) (Torres-Bugarín et al , 2004). We previously determined the frequency with which spontaneous micronucleated erythrocytes (MNE) appear in the species Aratinga canicularis (Zú ñiga- González et al , 2000).…”

Section: Introductionmentioning

confidence: 99%

Nuclear abnormalities in erythrocytes of parrots (Aratinga canicularis) related to genotoxic damage

et al. 2006

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Nuclear abnormalities in erythrocytes, as micronuclei and nuclear buds (BE), are considered potential biomarkers of genotoxic exposure. We described previously the frequency of spontaneous micronucleated erythrocytes (MNE) in the species Aratinga canicularis. Here, we have used this species to evaluate the induction of MNE and BE by mitomycin-C. Animals were given a single intracoelomic injection of 0, 2, 3 or 4 mg/kg mitomycin-C on two consecutive days. A drop of blood was obtained after 0, 24, 48 and 72 h, and stained smears were used to count micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes with buds (BPCE)/1000 polychromatic erythrocytes. The number of MNE and BE in 10 000 total erythrocytes was also counted. MNPCE and BPCE frequencies were elevated at 24, 48, and 72 h after the administration of the lower dose (P B/0.03). At a 3 mg/kg dose, the frequency of MNPCE increased at 48 and 72 h (P B/0.04) whereas the number of BPCE increased, but not significantly. Administration of 4 mg/kg mitomycin-C increased the number of MNE observed at 72 h (P B/0.03), the number of MNPCE at 48 h (P B/0.01) and 72 h (P B/0.006), the BE frequency at 72 h (P B/0.05), and the frequency of BPCE at 48 and 72 h (P B/0.001). While mitomycin-C appears to produce a parallel increase in MNPCE and BPCE frequencies, the MNE seemed to be a more sensitive indicator of genotoxicity than the BE. This suggests that evaluating BE and MNE in routine haematological analysis should be considered to evaluate environmental genotoxic exposure.

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Evaluation of cisplatin + 5-FU, carboplatin + 5-FU, and ifosfamide + epirubicine regimens using the micronuclei test and nuclear abnormalities in the buccal mucosa

Cited by 54 publications

References 23 publications

Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Danos cromossômicos e alterações nucleares em células esfoliadas do epitélio gengival de indivíduos com periodontite crônica moderada

Nuclear abnormalities in erythrocytes of parrots (Aratinga canicularis) related to genotoxic damage

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