Evaluation of clinical effectiveness of paclitaxel and ursolic acid co-loaded liposomes as enhanced treatment for head and neck squamous cell carcinoma

Lv, Bi-Hua; Tan, Wei; Shang, Xue‐Jun; Zhang, Ling

doi:10.4314/tjpr.v17i11.1

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…Likewise, it was observed that UA-PTX-LiP inhibited the growth of HSC-3 cancer cell line and increased cellular uptake that caused cell apoptosis more strongly than the chemotherapic administered alone or encapsulated. The more pronounced effect of the complex is believed to be due to the triterpene, ursolic acid, which is known to cause cancer cell apoptosis by inhibiting cyclooxygenase 2 [37].…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity Assessment of Liposomes Loaded With Biologically Active Substances on Oral Tumour Cells

PREDUT¹

2022

FARMACIA

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Lately, the scientific world has turned its attention to oral pathologies, observing the contribution of the health of the oral cavity to the general condition of the body. One of the most well-known diseases, oral carcinoma, is ranked worldwide as the sixth type of cancer in terms of frequency, and in Romania according to the 2020 statistics, it is on the 3rd place. The treatment generally consists in the administration of chemotherapy, hormonal therapy or surgical interventions, but these attract numerous adverse effects. Therefore, knowing the effectiveness of active principles extracted from plants in various pathologies, our research group wanted to incorporate betulin into liposomes to remove the inconveniences due to synthetic therapy. Likewise, encapsulation improves the solubility of the phytocompound. Morphological analysis revealed that the liposome-betulin system changes cell shape and confluence, the most visible effect being observed at 10 µM. MTT assay was performed to determine cell proliferation. Our results confirmed the activity of the liposome-betulin complex to inhibit cell viability from 75% at the lowest concentration (1 µM) to 29% at the highest concentration tested (10 µM). While betulin at 10 μM recorded a viability value of approximately 65%. In addition, the Hoechst assay established a dose-dependent tendency of betulin, and especially betulin incorporated into liposomes, to change shape and fragment cell nuclei. RezumatÎn ultimul timp, lumea științifică și-a îndreptat atenția spre patologiile de la nivel oral, observându-se contribuția sănătății cavității bucale la starea generală a organismului. Una dintre cele mai cunoscute afecțiuni, carcinomul oral, este clasat la nivel mondial ca fiind al șaselea tip de cancer ca și frecvență, iar în Romania conform statisticilor din 2020 se află pe locul 3. Tratamentul constă în general în administrarea de chimioterapice, terapie hormonală sau intervenții chirurgicale, însă aceastea atrag după sine numeroase efecte adverse. Prin urmare cunoscându-se eficacitatea principiilor active extrase din plante în diverse patologii, grupul nostru de cercetare a dorit încorporarea betulinei în lipozomi pentru a înlătura dezavantajele datorate terapiei de sinteză. În plus, încapsularea îmbunătățește solubilitatea fitocompusului. Analiza morfologică a relevat faptul că sistemul lipozombetulină modifică forma și confluența celulară, cel mai vizibil efect fiind observat la 10 µM. Analiza MTT a fost realizată pentru a determina proliferarea celulară. Rezultatele noastre au confirmat activitatea complexului lipozom-betulină de a inhiba viabilitatea celulară de la 75% la concentrația cea mai scăzută (1 µM) la 29% la cea mai mare concentrație testată (10 µM). Pe când betulina la 10 μM a înregistrat o valoare a viabilității de aproximativ 65%. În plus, testul Hoechst a stabilit tendința betulinei și mai ales a betulinei încorporate în lipozomi de a modifica forma și de a fragmenta nucleii celulari, dependent de doză.

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Section: Resultsmentioning

confidence: 99%

Cytotoxicity Assessment of Liposomes Loaded With Biologically Active Substances on Oral Tumour Cells

PREDUT¹

2022

FARMACIA

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“…The co-loading of UA was also experimented by Lv et al who combined the phytocompound with paclitaxel and loaded the mixture in stealth liposomes (UA-PTX-Lip) as potential treatment for head and neck squamous cell carcinoma. UA-PTX-Lip were prepared using the thin-film dispersion hydration method using hydrogenated soy phosphatidylcholine, cholesterol, and DSPE-PEG 2000 as lipid components; their anti-tumor activity was compared with paclitaxel liposomes (PTX-Lip) in vitro against HCS-3 cancer cells lines; the results showed that UA-PTX-Lip exhibited highly improved cytotoxicity on HCS-3 cells compared to PTX-Lip due to the presence of UA which exerts an increased oxidative effect on HSC-3 cells thus leading to an optimized apoptotic effect [279]. The combination drug obtained by linking UA and lamivudine (LMX) was prepared and evaluated for its anti-hepatitis B and hepatoprotective activity.…”

Section: Ursolic Acidmentioning

confidence: 99%

The Optimized Delivery of Triterpenes by Liposomal Nanoformulations: Overcoming the Challenges

Milan

Mioc

Prodea

et al. 2022

IJMS

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The last decade has witnessed a sustained increase in the research development of modern-day chemo-therapeutics, especially for those used for high mortality rate pathologies. However, the therapeutic landscape is continuously changing as a result of the currently existing toxic side effects induced by a substantial range of drug classes. One growing research direction driven to mitigate such inconveniences has converged towards the study of natural molecules for their promising therapeutic potential. Triterpenes are one such class of compounds, intensively investigated for their therapeutic versatility. Although the pharmacological effects reported for several representatives of this class has come as a well-deserved encouragement, the pharmacokinetic profile of these molecules has turned out to be an unwelcomed disappointment. Nevertheless, the light at the end of the tunnel arrived with the development of nanotechnology, more specifically, the use of liposomes as drug delivery systems. Liposomes are easily synthesizable phospholipid-based vesicles, with highly tunable surfaces, that have the ability to transport both hydrophilic and lipophilic structures ensuring superior drug bioavailability at the action site as well as an increased selectivity. This study aims to report the results related to the development of different types of liposomes, used as targeted vectors for the delivery of various triterpenes of high pharmacological interest.

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Self-assembled anticancer drug-loaded nanoparticles of hyaluronic acid conjugated with ursolic acid for enhanced combination chemotherapy

Kwon,

Kim,

et al. 2024

Journal of Industrial and Engineering Chemistry

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Evaluation of clinical effectiveness of paclitaxel and ursolic acid co-loaded liposomes as enhanced treatment for head and neck squamous cell carcinoma

Cited by 5 publications

References 28 publications

Cytotoxicity Assessment of Liposomes Loaded With Biologically Active Substances on Oral Tumour Cells

Cytotoxicity Assessment of Liposomes Loaded With Biologically Active Substances on Oral Tumour Cells

The Optimized Delivery of Triterpenes by Liposomal Nanoformulations: Overcoming the Challenges

Self-assembled anticancer drug-loaded nanoparticles of hyaluronic acid conjugated with ursolic acid for enhanced combination chemotherapy

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