Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro

Li, Yinglong; Sun, Ke; Ye, Guopin; Liang, Yi; Pan, Hong; Wang, Guomin; Zhao, Yijiao; Pan, Jie; Zhang, Jue; Fang, Jing

doi:10.1016/j.joen.2014.10.020

Cited by 67 publications

(68 citation statements)

References 39 publications

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“…However, the biosecurity of the plasma was still unknown. Li et al [14] have already reported that there was no significant difference in the microhardness and roughness of root canal dentin after treated with cold plasma for 12 min, that is, the mechanical properties of the canal dentin were not affected. However, modified nonequilibrium plasma and cold plasma are different after all even if their mechanism is similar.…”

Section: Discussionmentioning

confidence: 94%

“…appeared successionally in order to reduce harms to tissues while enhancing the sterilization effect. Studies have shown that atmospheric pressure cold plasma had a high efficiency in killing the bacteria in Enterococcus faecalis biofilms in in vitro root canal treatment [14,15] . Du et al [3] reported that modified nonequilibrium plasma with 2% CHX was more effective in disinfecting the in vitro bacterial biofilms than the conventional nonequilibrium plasma.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Chen

Shi

Qing

et al. 2016

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The aim of this study was to investigate the cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate (CHX) on human gingival fibroblasts (HGFs), and to evaluate the biosecurity of modified nonequilibrium plasma with 2% CHX as a new method of root canal treatment. Tissue samples taken from human gingiva were primarily cultured and passaged. Cells from passages 3-7 were used. HGFs were treated by modified nonequilibrium plasma with 2% CHX for 0 min (control group), 30 s, 1 min, 1.5 min, 3 min, 5 min, and 10 min, respectively, and then they were incubated for 0, 24, and 48 h. After that, cell counting kit-8 (CCK-8) assay was applied to analyze the cytotoxicity of modified nonequilibrium plasma with 2% CHX on HGFs. There was no significant difference between the 0 h group treated with the modified nonequilibrium plasma for 1 min and the control group (P>0.05). However, there were significant differences between all the other treated groups and the control group (P<0.05). When treated for 1.5 min or shorter, the cell viability was obviously increased; while treated for 3 min or longer, it was obviously reduced. Moreover, when successively cultured for 0, 24, and 48 h, cell viability was decreased at first and then increased in the 3-min-treated and 5-min-treated groups. The modified nonequilibrium plasma with 2% CHX was of no influence on cell viability in 1.5 min treatment, and it could be safely used on root canal treatment.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Chen

Shi

Qing

et al. 2016

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…Premixed argon (Ar) and oxygen (O 2 ) [Ar/O 2 (98%:2%; v/v); referred to as Ar/O 2 (2%) from here on] was used as the working gas with a flow rate of 5 l min −1 . More details can be found in our previous study . Ten millilitres of sterile distilled water was treated with non‐thermal plasma for 20 min to obtain PAW.…”

Section: Methodsmentioning

confidence: 99%

In vitro studies of the antimicrobial effect of non‐thermal plasma‐activated water as a novel mouthwash

Pan

et al. 2017

European J Oral Sciences

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The aim of this study was to evaluate the antimicrobial effects of non-thermal plasma-activated water (PAW) as a novel mouthwash in vitro. Three representative oral pathogens - Streptococcus mutans, Actinomyces viscosus and Porphyromonas gingivalis - were treated with PAW. The inactivation effect was evaluated using the colony-forming unit (CFU) method, and the morphological and structural changes of a cell were observed by scanning electron microscopy and transmission electron microscopy (TEM). The physicochemical properties of PAW were analysed, and its influence on the leakage of intracellular proteins and DNA was evaluated. The results showed significant reduction of Streptococcus mutans within 60 s, of Actinomyces viscosus within 40 s, and of Porphyromonas gingivalis in less than 40 s. Scanning electron microscopy and TEM images showed that the normal cell morphology changed by varying degrees after treatment with PAW. Intracellular proteins (280 nm) and DNA (260 nm) leaked from all three species of bacteria after treatment with PAW. Reactive oxygen species (ROS), especially atomic oxygen (O), hydroxyl radical (˙OH), and hydrogen peroxide (H O ), were generated and led to strong oxidative stress and cell damage. These results suggest that PAW has potential use as a novel antimicrobial mouthwash.

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“…1) 11,12) . However, there are few reports about its application for sterilization in the field of dentistry [13][14][15][16] . Yamazaki et al 17) have previously reported the sterilization effects of plasma exposure in an acidic environment (the reduced-pH method) for a wide variety of oral pathogenic microorganisms, both in suspension and in biofilms.…”

Section: Introductionmentioning

confidence: 99%

Plasma-treated water eliminates <i>Streptococcus mutans</i> in infected dentin model

Tasaki

Ohshima

Usui

et al. 2017

Dental Materials Journal

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Non-mechanical procedures for removing caries-infected dentin are warranted in dentistry. We previously demonstrated the marked sterilization effect for direct irradiation of low-temperature plasma using dentin model infected with Streptococcus mutans. However it requires 180 s of intraoral plasma irradiation to eliminate bacteria. We alternatively investigated whether plasma-treated water (PTW), i.e., pure water exposed to plasma in an atmosphere, has a same bactericidal activity with the plasma irradiation. In the infected dentin model, the viable S. mutans counts recovered by bur at depth of 0.8-2.4 mm from the cavity floor were 10 4 -10 6 CFU/ round bur. After PTW application for only 10 s, the count was significantly decreased to below the detection limit (2.5 CFU/round bur) or 3.0±5.0 CFU/round bur. Since the bactericidal activity of PTW is rapidly deactivated at body temperature (37°C), PTW is likely to be biocompatible and holds significant potential for non-mechanical procedures for removing caries-infected dentin.

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Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro

Cited by 67 publications

References 39 publications

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

In vitro studies of the antimicrobial effect of non‐thermal plasma‐activated water as a novel mouthwash

Plasma-treated water eliminates <i>Streptococcus mutans</i> in infected dentin model

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