Evaluation of Collagen and Elastin Content in Skin of Multiparous Minks Receiving Feed Contaminated with Deoxynivalenol (DON, vomitoxin) with or without Bentonite Supplementation

Abstract

Deoxynivalenol (DON, vomitoxin) is considered one of the most dangerous mycotoxins contaminating cereal products for food and feed. One of the protective methods against the adverse effect of DON on mink health is to use a component such as bentonite as a feed supplement to allow toxins absorption. The aim of this study was to determine the effect of DON, administered alone or with bentonite, on the histological structure of the skin and the content of collagen and elastin. A multiparous minks from control group (not exposed to DON) and a study groups receiving fed with DON-containing wheat for seven months: I: at a concentration of 1.1 mg/kg of feed, II: at a concentration of 3.7 mg/kg, III: DON at a concentration of 3.7 mg/kg and bentonite at a concentration of 0.5 kg/1000 kg of feed (0.05%) and IV: DON at a concentration of 3.7 mg/kg and bentonite at a concentration 2 kg/1000 kg (0.2%). After performing euthanasia and before pelting, skin samples of 2 cm in diameter were drawn from the multiparous minks from the lateral surface of the right anterior limb. Our obtained results clearly indicate that DON administered for a period of seven months at a dose of 1.1 mg/kg significantly changes the thickness of skin of a multiparous mink. It causes an increase in the percentage of elastin from 5.9% to 9.4% and a decrease in immature collagen, which results in a change in the collagen/elastin ratio from 10/1 to 5/1. A dose of 3.7 mg/kg DON in feed without or with 0.05% bentonite causes the absence of immature collagen in the dermis, but the addition of 0.2% bentonite in the feed reveals the presence of immature collagen and increase the percentage of the elastin.

The Influence of Fusarium Mycotoxins on the Liver of Gilts and Their Suckling Piglets

“…Our finding is in contrast with previous studies because in those cases, experimental animals had an increased amount of interlobular connective tissue in the liver [ 27 , 30 , 34 ]. A recent study on collagen and elastin content in skin of mink receiving DON-contaminated feed showed a decrease in type III (immature) collagen when mink received DON at a concentration of 1.1 mg/kg of feed and complete absence of type III (immature) collagen when mink received a dose of 3.7 mg/kg DON in feed with or without 0.05% bentonite [ 47 ]. As interlobular connective tissue in pig liver contains both type I and type III collagen [ 48 ], the observed decrease in interlobular connective tissue in our study may have been due to DON influencing the expression of fibrous collagens.…”

Treatment with synchronized radiofrequency and facial muscle stimulation: Histologic analysis of human skin for changes in collagen and elastin fibers

“…Following the administration of the therapies, there was a twofold increase in elastin levels, resulting in the occupation of 7% of the slice area. This observation indicates a restoration of skin elastin levels to a state consistent with healthy physiological levels 14–16 . Additionally, as SMAS is a tissue that is intertwined with the rest of the facial structures and is made of collagen and elastin, we believe the SMAS layer was also affected and helped with the results 17 .…”

A Glance into the Destiny of Transcriptomic Activity, Embodied by the HOX Genes, in Neonatal and Aging Dermal Cells

“…The decrease of collagen and elastin fibers is a typical response and bio indicator of skin aging. [ 28 ] Thus, we also investigated the representative component of the skin that plays a significant role in maintaining its health and integrity: collagen type I ( COL1A1 and COL1A2 ), collagen type III ( COL3A1 ), collagen type V ( COL5A1 , COL5A2 , and COL5A3 ), and elastin fibers ( ELN , FBLN1 , and FBLN2 ) ( Figure 5 A). As shown in Figure 5A, all nine genes had age‐dependent different expression patterns in the HEKs and the HDFs and had higher gene expression activities in the HDFs compared to the HEKs.…”