Evaluation of Collagen Building, UV Protective and Antioxidant Properties of CollaBZen on Human Collagen Type I,II and Type IV

Abstract

CollaBZen TM from Zenherb labs, a proprietary blend of polyphenols extracted from Phyllanthus emblica, Camellia sinensis, and Coffea arabica, was assessed for its anti-ageing potential. Human dermal fibroblast and chondrocyte cells were exposed in vitro to hydrogen peroxide and UVA to induce ageing manifested as ROS production and collagen breakdown respectively. CollaBZen TM revealed no cytotoxicity up to 0.16 mg/ml in both NHDF and HCH cells. DCFDA assay revealed that CollaBZen TM demonstrated a dose-dependent reduction in H2O2-induced ROS levels in both cell types. Exposure to UVA rays led to collagen depletion but CollaBZen TM pre-treatment showed higher collagen levels, offering protection. At 0.04 mg/ml, it conferred a significant shield against UVA rays, with a 48.13%, 39.47%, and 30.5% increase in COL1A1, COL4A1, and COL2A1, respectively. Beyond promoting collagen as documented in the previous studies, CollaBZen TM emerges as a potent food supplement safeguarding skin and joint cells from ageing consequences, reinforcing its role in agerelated preventive care.