The work was carried out in 2018-2019 in the forest-steppe zone of Samara region. The purpose of the research is to conduct study samples of collection nursery of various ecological and geographical origins and identify new sources of economically valuable traits for breeding in the forest – steppe of the Middle Volga region. The material for research was 352 collection samples of spring soft wheat, including 108 foreign varieties and hybrids and 244 domestic samples of breeding institutions of the Russian Federation. Meteorological conditions during the research years were quite severe, and vegetation took place in arid and acutely arid conditions (hydrothermal coefficient in 2018 – 0.51, in 2019 – 0.48, with a long-term value in the region of 0.73). The greatest variability over the years of research was observed in the indicators of grain yield of samples (Сv=23.9...27.5 %) and plants height (Сv=10.8...12.9 %). According to the research results, 30 new genetic sources of economically valuable traits of spring soft wheat were identified. Sources of precocity (period of shoots-earing 35...37 days): Uralskaya kukushka, Chelyaba rannyaya, Tyumenskaya 25, Lutescens 70, Odeta, Libertina, Chi Mai, Long Fu 7, M83-1551. Sources of short stems (plants height 40...45 cm): KWS Torridon, KWS Jetstream, Florens, Eleganza, Long Fu 13. Sources of consistently high productivity (grain yield 415...554 g/m2, and the yield increase over the standard 71...210 g/m2): Erythrospermum 4089, Grekum 5523, Lutescens 6029, Lutescens 6074/6-23, Lutescens 6102/1-32, Lutescens 6102/1-34 Erythrospermum 6517/24-1, Ekada 214, Burlak, Ulyanovskaya 105, Lebedushka, Saratovskaya 73. Sources of high protein content (15.4...16.7 %) and gluten (36.0...42.0 %) in grain: Erythrospermum 3898, Erythrospermum 4112, Erythrospermum 4171, Erythrospermum 5289