Evaluation of combination therapy with hydrocortisone, vitamin C, and vitamin E in a rat model of intestine ischemia-reperfusion injury

Tavasoli, Mehdi; Azari, Omid; Kheirandish, Reza; Abbasi, Mohammad Farajli

doi:10.1007/s00580-017-2610-4

Cited by 8 publications

(6 citation statements)

References 48 publications

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“…One of the most promising candidates to revert GCR is the antioxidant L-ascorbic acid, or vitamin C. Vitamin C has been shown to restore the GR function by reverting the effect of oxidative stress on GR's ligand- and DNA-binding capacity ( 98 ). Preclinical studies demonstrated the synergistic effect of adding vitamin C to GC therapy on endothelial barrier function ( 99 ) and intestinal mucosa injuries in sepsis models ( 100 ). Furthermore, two recent retrospective before–after clinical trials evaluating this combination therapy in the treatment of septic shock and severe pneumonia look very promising ( 101 , 102 ).…”

Section: Dysfunction Of the Hpa Axis During Sepsismentioning

confidence: 99%

Glucocorticoids in Sepsis: To Be or Not to Be

Vandewalle

Libert

2020

Front. Immunol.

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Section: Dysfunction Of the Hpa Axis During Sepsismentioning

confidence: 99%

Glucocorticoids in Sepsis: To Be or Not to Be

Vandewalle

Libert

2020

Front. Immunol.

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“…Barabutis et al have demonstrated that hydrocortisone together with vitamin C protects the vascular endothelium from damage by endotoxin, while neither agent alone had this effect [ 113 ]. Azari et al compared the protective effects of Vitamin C (in a dose of 50 mg/kg), vitamin E and hydrocortisone alone and in combination, in a murine renal and intestinal ischemia-reperfusion model [ 114 , 115 ]. In these studies, both vitamin C and hydrocortisone reduced the ischemia-reperfusion injury with the combination having synergistic protective effects.…”

Section: Hydrocortisone Ascorbic Acid and Thiamine (Hat) In Combmentioning

confidence: 99%

Hydrocortisone, Ascorbic Acid and Thiamine (HAT Therapy) for the Treatment of Sepsis. Focus on Ascorbic Acid

Marik

2018

Nutrients

103

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Sepsis is a devastating disease that carries an enormous toll in terms of human suffering and lives lost. Over 100 novel pharmacologic agents that targeted specific molecules or pathways have failed to improve the outcome of sepsis. Preliminary data suggests that the combination of Hydrocortisone, Ascorbic Acid and Thiamine (HAT therapy) may reduce organ failure and mortality in patients with sepsis and septic shock. HAT therapy is based on the concept that a combination of readily available, safe and cheap agents, which target multiple components of the host’s response to an infectious agent, will synergistically restore the dysregulated immune response and thereby prevent organ failure and death. This paper reviews the rationale for HAT therapy with a focus on vitamin C.

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“…In the present study, HC also ameliorated other parameters of intestinal injury namely architectural distortion and villous blunting. The protective effect of HC against mucosal injury has been previously demonstrated in intestinal ischemia-reperfusion (17). Most importantly, the intestinal mechanical barrier was strengthened by HC administration through increased enterocytes’ occludin and claudin-1 expression, thus restricting the passage of intraluminal pathogens and/or their products (endotoxin) through the paracellular space.…”

Section: Discussionmentioning

confidence: 86%

Fecal Microbiota Transplantation and Hydrocortisone Ameliorate Intestinal Barrier Dysfunction and Improve Survival in a Rat Model of Cecal Ligation and Puncture-Induced Sepsis

Assimakopoulos

Papadopoulou

Bantouna

et al. 2020

Shock

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Introduction: Sepsis is a life-threatening syndrome which can progress to multiple organ dysfunction with high mortality. Intestinal barrier failure exerts a central role in the pathophysiological sequence of events that lead from sepsis to multiple organ dysfunction. The present study investigated the role of hydrocortisone (HC) administration and fecal microbiota transplantation (FMT) in several parameters of the gut barrier integrity, immune activation, and survival, in a model of polymicrobial sepsis in rats. Methods: Forty adults male Wistar rats were randomly divided into four groups: sham (group I), cecal ligation and puncture (CLP) (group II), CLP þ HC (2.8 mg/kg, intraperitoneally single dose at 6 h) (group III), and CLP þ FMT at 6 h (group IV). At 24 h post-CLP, ileal tissues were harvested for histological and immunohistochemical analyses while endotoxin, IL-6, and IL-10 levels in systemic circulation were determined. In a second experiment the same groups were observed for 7 days for mortality, with daily administration of hydrocortisone (group III) and FMT (group IV) in surviving rats. Results: HC administration and FMT significantly reduced mortality of septic rats by 50%. These interventions totally reversed intestinal mucosal atrophy by increasing villous density and mucosal thickness (mm, mean AE SD:

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Evaluation of combination therapy with hydrocortisone, vitamin C, and vitamin E in a rat model of intestine ischemia-reperfusion injury

Cited by 8 publications

References 48 publications

Glucocorticoids in Sepsis: To Be or Not to Be

Glucocorticoids in Sepsis: To Be or Not to Be

Hydrocortisone, Ascorbic Acid and Thiamine (HAT Therapy) for the Treatment of Sepsis. Focus on Ascorbic Acid

Fecal Microbiota Transplantation and Hydrocortisone Ameliorate Intestinal Barrier Dysfunction and Improve Survival in a Rat Model of Cecal Ligation and Puncture-Induced Sepsis

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