2011
DOI: 10.1016/j.domaniend.2011.05.001
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Evaluation of commercially available assays for the measurement of equine insulin

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Cited by 62 publications
(76 citation statements)
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“…For example, the sample with the lowest concentration yielded a value of 50.72 µU/mL when LC-MS was used, 17.53 µU/mL when the RIA was used, and 8.12 µU/mL when the ELISA was used. 20 The sample with the highest concentration yielded a value of 454.16 µU/mL via LC-MS, 220.58 µU/mL via RIA, and 35.97 µU/mL via ELISA. 20 Overall, the RIA underestimated concentrations measured by LC-MS by 2.8-fold and the ELISA underestimated concentrations measured by LC-MS by 8.3-fold.…”
Section: Discussionmentioning
confidence: 95%
See 1 more Smart Citation
“…For example, the sample with the lowest concentration yielded a value of 50.72 µU/mL when LC-MS was used, 17.53 µU/mL when the RIA was used, and 8.12 µU/mL when the ELISA was used. 20 The sample with the highest concentration yielded a value of 454.16 µU/mL via LC-MS, 220.58 µU/mL via RIA, and 35.97 µU/mL via ELISA. 20 Overall, the RIA underestimated concentrations measured by LC-MS by 2.8-fold and the ELISA underestimated concentrations measured by LC-MS by 8.3-fold.…”
Section: Discussionmentioning
confidence: 95%
“…20 The sample with the highest concentration yielded a value of 454.16 µU/mL via LC-MS, 220.58 µU/mL via RIA, and 35.97 µU/mL via ELISA. 20 Overall, the RIA underestimated concentrations measured by LC-MS by 2.8-fold and the ELISA underestimated concentrations measured by LC-MS by 8.3-fold. 20 These findings are consistent with those of the present study, suggesting that measured concentrations when the RIA is used are much higher than when the equine-specific ELISA is used.…”
Section: Discussionmentioning
confidence: 95%
“…Plasma insulin concentrations were measured using an ELISA kit previously validated for use in Equidae (Tinworth et al, 2011;Mercodia Insulin ELISA;Mercodia). The intra-assay coefficient of variation (CV) was 7% (24.1 mU/L).…”
Section: Combined Glucose Insulin Tolerance Test (Cgit)mentioning
confidence: 99%
“…The commonly available laboratory methodologies for insulin determination in equine blood use either human or porcine diagnostic assay techniques (with validation), based on the fact that the molecular structure of insulin is highly conserved across mammalian species. 54 A study applied identical validation protocols to all of the insulin immunoassays currently available in order to identify which ones were accurate and reliable for measuring equine insulin concentrations in equine plasma samples. 54 In that study, several commonly used assays were shown to perform poorly, but the authors identified a commercially available radioimmunoassay method based on recombinant human insulin that performed satisfactorily.…”
Section: Diagnostic Approach For Insulin Resistance and Diabetes In Hmentioning
confidence: 99%