Evaluation of complementary effects of 9,10-anthraquinone and fumaric acid on methanogenesis and ruminal fermentation<i>in vitro</i>

Ebrahimi, Seyed Hadi; Mohini, Madhu; Singhal, K. K.; Miri, Vahideh Heidarian; Tyagi, Amrish Kumar

doi:10.1080/1745039x.2011.594345

Cited by 16 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tatsuoka et al ( 2008 ) and Ebrahimi et al ( 2011 ) could decrease H 2 accumulation in methanogenesis-inhibited ruminal batch cultures by adding fumarate or malate. Whilst the addition of a substrate or a fermentation intermediate might remove or decrease the substrate kinetics limitation of a process, it could at the same time make it thermodynamically feasible.…”

Section: Discussionmentioning

confidence: 99%

Limits to Dihydrogen Incorporation into Electron Sinks Alternative to Methanogenesis in Ruminal Fermentation

Ungerfeld

2015

Front. Microbiol.

View full text Add to dashboard Cite

Research is being conducted with the objective of decreasing methane (CH4) production in the rumen, as methane emissions from ruminants are environmentally damaging and a loss of digestible energy to ruminants. Inhibiting ruminal methanogenesis generally results in accumulation of dihydrogen (H2), which is energetically inefficient and can inhibit fermentation. It would be nutritionally beneficial to incorporate accumulated H2 into propionate or butyrate production, or reductive acetogenesis. The objective of this analysis was to examine three possible physicochemical limitations to the incorporation of accumulated H2 into propionate and butyrate production, and reductive acetogenesis, in methanogenesis-inhibited ruminal batch and continuous cultures: (i) Thermodynamics; (ii) Enzyme kinetics; (iii) Substrate kinetics. Batch (N = 109) and continuous (N = 43) culture databases of experiments with at least 50% inhibition in CH4 production were used in this meta-analysis. Incorporation of accumulated H2 into propionate production and reductive acetogenesis seemed to be thermodynamically feasible but quite close to equilibrium, whereas this was less clear for butyrate. With regard to enzyme kinetics, it was speculated that hydrogenases of ruminal microorganisms may have evolved toward high-affinity and low maximal velocity to compete for traces of H2, rather than for high pressure accumulated H2. Responses so far obtained to the addition of propionate production intermediates do not allow distinguishing between thermodynamic and substrate kinetics control.

show abstract

Section: Discussionmentioning

confidence: 99%

Limits to Dihydrogen Incorporation into Electron Sinks Alternative to Methanogenesis in Ruminal Fermentation

Ungerfeld

2015

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Through adding propionate formation intermediates, Tatsuoka et al (2008) and Ebrahimi et al (2011) had some success at decreasing H 2 accumulation in methanogenesis-inhibited ruminal batch incubations, which would indicate, that at least under their conditions, propionate formation was limited by thermodynamics or substrate kinetics (through the availability of a C skeleton). Fonty et al (2007) found that addition of fumarate to a methanogen-free ruminal incubation accelerated H 2 utilization without affecting its final point at 24 h, which would suggest a limitation of a substrate kinetic nature.…”

Section: Discussionmentioning

confidence: 99%

A theoretical comparison between two ruminal electron sinks

Ungerfeld

2013

Front. Microbiol.

View full text Add to dashboard Cite

Dihydrogen accumulation resulting from methanogenesis inhibition in the rumen is an energy loss and can inhibit fermentation. The objective of this analysis was to compare the energetic and nutritional consequences of incorporating H2 into reductive acetogenesis or additional propionate production beyond the acetate to propionate shift occurring along with methanogenesis inhibition. Stoichiometric consequences were calculated for a simulated fermentation example. Possible nutritional consequences are discussed. Incorporating H2 into reductive acetogenesis or additional propionate production resulted in equal heat of combustion output in volatile fatty acids (VFA). Incorporation of H2 into reductive acetogenesis could result in moderate decrease in ruminal pH, although whole-animal buffering mechanisms make pH response difficult to predict. Research would be needed to compare the microbial protein production output. There could be post-absorptive implications due to differences in VFA profile. Electron incorporation into reductive acetogenesis could favor energy partition toward milk, but increase risk of ketosis in high-producing dairy cows on ketogenic diets. Greater propionate production could favor milk protein production, but may be less desirable in animals whose intake is metabolically constrained, like feedlot steers. Because of the different nutritional implications, and because practical solutions to incorporate H2 into either pathway are not yet available, it is recommended to research both alternatives.

show abstract

“…When methanogenesis was simultaneously inhibited with a chemical compound, increased availability of [H] not incorporated into CH 4 favored the conversion of the added carboxylic acids to the expected end products. In the presence of inhibitors of methanogenesis, the addition of propionate precursors malate (Mohammed et al, 2004) or fumarate (Tatsuoka et al, 2008;Ebrahimi et al, 2011) increased propionate production in vitro 1 Greater ranges in H 2 concentration in some studies in which methanogenesis was not inhibited are due to multiple measurements throughout the day in animals fed once or twice a day, rather than to variation caused by treatments imposed.…”

Section: The Competition For Dihydrogenmentioning

confidence: 99%

Metabolic Hydrogen Flows in Rumen Fermentation: Principles and Possibilities of Interventions

2020

View full text Add to dashboard Cite

Rumen fermentation affects ruminants productivity and the environmental impact of ruminant production. The release to the atmosphere of methane produced in the rumen is a loss of energy and a cause of climate change, and the profile of volatile fatty acids produced in the rumen affects the post-absorptive metabolism of the host animal. Rumen fermentation is shaped by intracellular and intercellular flows of metabolic hydrogen centered on the production, interspecies transfer, and incorporation of dihydrogen into competing pathways. Factors that affect the growth of methanogens and the rate of feed fermentation impact dihydrogen concentration in the rumen, which in turn controls the balance between pathways that produce and incorporate metabolic hydrogen, determining methane production and the profile of volatile fatty acids. A basic kinetic model of competition for dihydrogen is presented, and possibilities for intervention to redirect metabolic hydrogen from methanogenesis toward alternative useful electron sinks are discussed. The flows of metabolic hydrogen toward nutritionally beneficial sinks could be enhanced by adding to the rumen fermentation electron acceptors or direct fed microbials. It is proposed to screen hydrogenotrophs for dihydrogen thresholds and affinities, as well as identifying and studying microorganisms that produce and utilize intercellular electron carriers other than dihydrogen. These approaches can allow identifying potential microbial additives to compete with methanogens for metabolic hydrogen. The combination of adequate microbial additives or electron acceptors with inhibitors of methanogenesis can be effective approaches to decrease methane production and simultaneously redirect metabolic hydrogen toward end products of fermentation with a nutritional value for the host animal. The design of strategies to redirect metabolic hydrogen from methane to other sinks should be based on knowledge of the physicochemical control of rumen fermentation pathways. The application of new -omics techniques together with classical biochemistry methods and mechanistic modeling can lead to exciting developments in the understanding and manipulation of the flows of metabolic hydrogen in rumen fermentation.

show abstract

Evaluation of complementary effects of 9,10-anthraquinone and fumaric acid on methanogenesis and ruminal fermentationin vitro

Cited by 16 publications

References 28 publications

Limits to Dihydrogen Incorporation into Electron Sinks Alternative to Methanogenesis in Ruminal Fermentation

Limits to Dihydrogen Incorporation into Electron Sinks Alternative to Methanogenesis in Ruminal Fermentation

A theoretical comparison between two ruminal electron sinks

Metabolic Hydrogen Flows in Rumen Fermentation: Principles and Possibilities of Interventions

Contact Info

Product

Resources

About