Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture

Batista, Fabiana Regina Xavier; Pereira, Carlos A.; Mendonça, Ronaldo Zucatelli; Moraes, Ângela Maria

doi:10.2225/vol9-issue5-fulltext-17

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…The Drosophila expression system has been available for several years, since these cells are able to synthesize proteins with adequate post-translational modifications [25]. The culture of Drosophila cells can be performed in several media, but serum-free medium is recommended, due to the cost, lot-to-lot variability and downstream processing difficulties associated to the use of serum-supplemented media [19,[26][27][28][29]. Moreover, regulatory agencies such as the European Medicine Evaluation Agency and the Food and Drug Administration (USA) encourage biological compounds manufacturers to reduce or eliminate the use of substances of animal origin [30].…”

Section: Introductionmentioning

confidence: 99%

Behavior of Wild-type and Transfected S2 Cells Cultured in Two Different Media

Batista

Greco

Astray

et al. 2010

Appl Biochem Biotechnol

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An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprotein from rabies virus (RVGP) and S2AcHBsAgHy-9C expressing the surface antigen of hepatitis B virus (HBsAg), both obtained from Drosophila melanogaster S2 cells. Growth of wild-type S2 cells was also evaluated in the same medium. Cell behavior in the tested medium was compared to that verified in Sf900 II®. The results show that in shake flasks, S2AcRVGP2k and S2AcHBsAgHy-9C cells reached around 2 × 10(7) cells mL(-1) in both media. In supplemented IPL-41 and Sf900 II® media, S2AcRVGP2k cells produced 367 ng RVGP mL(-1) and 638 ng RVGP mL(-1), respectively, while S2AcHBsAgHy-9C cells correspondently produced 573 ng HBsAg mL(-1) and 322 ng HBsAg mL(-1) in the mentioned media. In stirred tanks, S2AcRVGP2k cells reached 3 × 10(7) cells mL(-1) and produced up to 758 ng RVGP mL(-1). In general, glucose was consumed by cells, while lactate and ammonia were produced.

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Section: Introductionmentioning

confidence: 99%

Behavior of Wild-type and Transfected S2 Cells Cultured in Two Different Media

Batista

Greco

Astray

et al. 2010

Appl Biochem Biotechnol

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“…Os dados necessários para a formulação de modelo cinético do cultivo de células S2, Drosophila melanogaster, estavelmente transformadas com vetores plasmidiais contendo o gene da glicoproteína G do vírus da raiva (pAcGPV), foram obtidos por três alunos de doutorado e um de mestrado e estão descritos em Batista et al (2006), Batista (2007), Batista et al (2008), Batista et al(2009a), Galesi (2007), Galesi;Moraes (2007), , Swiech (2007), Swiech et al(2008a,b,c) Posteriormente, a subpopulação S2AcGPV2-KSF900 foi adaptada ao meio TC100-AG e recebeu a denominação S2AcGPV2-KTC100 (AGUIAR et al, 2009;AGUIAR, 2010).…”

Section: Materiais E Métodosunclassified

“…O meio SF900 II® utilizado por Swiech (2007), Swiech et al (2008a,b,c) é também um meio de cultura livre de soro fetal bovino comercializado pela GIBCO (USA). O meio utilizado por Batista (2007) e Batista et al (2006Batista et al ( , 2008Batista et al ( , 2009a…”

Section: Meios De Culturaunclassified