Evaluation of Consolidated Bioprocessing of Sugarcane Biomass by a Multiple Hydrolytic Enzyme Producer Saccharomyces Yeast

From Saccharomyces cerevisiae to Ethanol: Unlocking the Power of Evolutionary Engineering in Metabolic Engineering Applications

“…Most importantly, direct conversion of lignocellulosic substrates to ethanol was achieved with that strain, without prior high-cost enzyme treatment. This enabled SSF applications with the engineered AC14 strain, leading to consolidating bioprocessing (CBP), as reported by Perez et al [143]. CBP is a promising and low-cost, emerging strategy that reduces enzyme production, biomass hydrolysis, and sugars fermentation to a single step in a reactor.…”

Co-Fermentation of Glucose–Xylose–Cellobiose–XOS Mixtures Using a Synthetic Consortium of Recombinant Saccharomyces cerevisiae Strains

“…Claes et al reported a recombinant S. cerevisiae strain AC14 using industrial Ethanol Red TM as chassis, capable of secreting seven different lignocellulosic enzymes and fermenting xylose and glucose [58]. Then, the consolidated bioprocessing profile of the AC14 strain was investigated in synthetic media composed of approximately 16 g L −1 xylose, 11 g L −1 glucose, 12 g L −1 cellobiose, 6 g L −1 corncob xylan, 20 g L −1 peptone, and 10 g L −1 yeast extract [27]. Glucose, xylose, and cellobiose were consumed within 6 h, while xylan hydrolysis was slightly slower; all potential sugars were consumed after 30 h. Considering a process duration of 6 h, where the majority of the potential sugars had been released and fermented (92%), ethanol titer, productivity, and yield obtained were 26.8 g L −1 , 4.46 g L −1 h −1 , and 0.5 g g −1 , respectively.…”

“…So far, research on the fermentation of LB hydrolysates by S. cerevisiae has focused on the development of 'generalist' yeast strains capable of fermenting mixtures of two or three kinds of LB-derived sugars [26,27]. The current lack of simultaneous mixed-sugar utilization, caused by negative factors such as metabolic burden, limits achievable titers, yields, and productivities.…”

Impacto Da Agitação No Bioprocessamento Consolidado Do Bagaço De Cana Utilizando Levedura Recombinante

“…As atividades de celulases totais, β-glucosidase, β-xilosidase e xilanases foram quantificadas no meio fermentado (sobrenadante após a remoção das células, denominado extrato enzimático) de acordo com metodologias detalhadas por Perez et al (2023). A atividade enzimática das células totais foi baseada na liberação de glicose do papel de filtro Whatmam n° 1 (15 mm diâmetro) a 50 °C e pH 5,0; β-glicosidase foi medida pela liberação de glicose a 50 °C e pH 5,5 a partir de celobiose 2%; a atividade da xilanase foi medida pela velocidade inicial da hidrólise de xilana de faia 1% a 50ºC e pH 5,5; e a atividade da β-xilosidase foi medida pela liberação de 4-nitrofenol (ε = 1950 mol/L/cm) durante a hidrólise de 4-nitrofenil β-D-xilopiranósido (p-NPX) a 50 °C e pH 5,5.…”

“…Neste sentido, o Bioprocessamento Consolidado (BPC) se destaca como uma tecnologia emergente, na qual a produção de enzimas, hidrólise da biomassa e fermentação dos açúcares liberados ocorrem simultaneamente em um único reator (PEREZ et al, 2023). O BPC se caracteriza pelo uso de microrganismos ou consórcio de microrganismos (fungos ou bactérias) selvagens ou recombinantes, que apresentem tanto a capacidade de secreção de enzimas hidrolíticas quanto de transformação dos açúcares liberados nos produtos de interesse, dispensando, desta forma, a necessidade dos preparados enzimáticos comerciais (OLGUIN-MACIEL et al, 2020).…”