Under the new situation, the rapid development of the petroleum industry has increasingly required the quality and quantity of chemical talents in oil fields. "Oil and Gas Field Applied Chemistry Experiment" is an extremely important part of Xi'an Shiyou University's applied chemistry training program. It plays the role of a bridge between theoretical learning and applied practice, and this course is of great importance to students of applied chemistry. In order to improve the teaching quality of Oil and Gas Field Applied Chemistry Experiment, this article takes Xi'an Shiyou University as an example. Based on the teaching experience of Oil and Gas Field Applied Chemistry Experiment in recent years, the author analysed the problems of academic performance and composition about the teachers, teaching materials, instruments and students, and corresponding practical explorations are put forward. It is expected to provide a certain theoretical basis for the teaching reform of the course of "Oil and Gas Field Applied Chemistry Experiment " of our school, and provide some reference for related disciplines of domestic petroleum colleges.