Evaluation of CTB-sLip for Targeting Lung Metastasis of Colorectal Cancer

Zhang, Xiaoying; Tang, Wenjing; Wen, Haoyu; Wu, Ercan; Ding, Tianhao; Gu, Jie; Lv, Zhongwei; Zhan, Changyou

doi:10.3390/pharmaceutics14040868

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…FAM-loaded sLip (sLip/FAM) and FAM-loaded IR783-sLip (IR783-sLip/FAM) were prepared based on the same approach. Specifically, FAM was dissolved in sterile saline (65 °C, pH 7.0) at first, followed by the same procedure as outlined above, with the final removal of free FAM using a Sephadex G50 column [ 38 ]. The FAM concentration was measured under conditions of excitation (Ex) at 494 nm and emission (Em) at 580 nm using a Spark Cyto automatic microplate reader (Tecan Group Ltd., Männedorf, Zurich, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Effects of Surface IR783 Density on the In Vivo Behavior and Imaging Performance of Liposomes

Long,

Zhao,

Gao

et al. 2024

Pharmaceutics

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Background: Nanoparticles conjugated with fluorescent probes have versatile applications, serving not only for targeted fluorescent imaging but also for evaluating the in vivo profiles of designed nanoparticles. However, the relationship between fluorophore density and nanoparticle behavior remains unexplored. Methods: The IR783-modified liposomes (IR783-sLip) were prepared through a modified ethanol injection and extrusion method. The cellular uptake efficiency of IR783-sLip was characterized by flow cytometry and fluorescence microscope imaging. The effects of IR783 density on liposomal in vivo behavior were investigated by pharmacokinetic studies, biodistribution studies, and in vivo imaging. The constitution of protein corona was analyzed by the Western blot assay. Results: Dense IR783 modification improved cellular uptake of liposomes in vitro but hindered their blood retention and tumor imaging performance in vivo. We found a correlation between IR783 density and protein corona absorption, particularly IgM, which significantly impacted the liposome performance. Meanwhile, we observed that increasing IR783 density did not consistently improve the effectiveness of tumor imaging. Conclusions: Increasing the density of modified IR783 on liposomes is not always beneficial for tumor near-infrared (NIR) imaging yield. It is not advisable to prematurely evaluate novel nanomaterials through fluorescence dye conjugation without carefully optimizing the density of the modifications.

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Section: Methodsmentioning

confidence: 99%

Effects of Surface IR783 Density on the In Vivo Behavior and Imaging Performance of Liposomes

Long,

Zhao,

Gao

et al. 2024

Pharmaceutics

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“…Human-derived colorectal cancer cell lines demonstrated high binding affinity and cellular uptake with CTB-sLip. Moreover, these nanosystems showed elevated targeting capability for the lung metastasis of colorectal cancer in a model of nude mice in comparison to pegylated liposomes without CtxB modification [ 147 ]. CtxB applications are summarized in Table 4 .…”

Section: Biomedical Applications Of the Ab Toxinsmentioning

confidence: 99%

AB Toxins as High-Affinity Ligands for Cell Targeting in Cancer Therapy

Márquez-López,

Fanarraga

2023

IJMS

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Conventional targeted therapies for the treatment of cancer have limitations, including the development of acquired resistance. However, novel alternatives have emerged in the form of targeted therapies based on AB toxins. These biotoxins are a diverse group of highly poisonous molecules that show a nanomolar affinity for their target cell receptors, making them an invaluable source of ligands for biomedical applications. Bacterial AB toxins, in particular, are modular proteins that can be genetically engineered to develop high-affinity therapeutic compounds. These toxins consist of two distinct domains: a catalytically active domain and an innocuous domain that acts as a ligand, directing the catalytic domain to the target cells. Interestingly, many tumor cells show receptors on the surface that are recognized by AB toxins, making these high-affinity proteins promising tools for developing new methods for targeting anticancer therapies. Here we describe the structure and mechanisms of action of Diphtheria (Dtx), Anthrax (Atx), Shiga (Stx), and Cholera (Ctx) toxins, and review the potential uses of AB toxins in cancer therapy. We also discuss the main advances in this field, some successful results, and, finally, the possible development of innovative and precise applications in oncology based on engineered recombinant AB toxins.

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“…Zhang and his team developed PEGylated liposomes (CTB-sLip) coupled with cholera toxin subunit b protein. These CTB-sLips showed excellent affinity and cellular uptake in both human-derived CRC cell lines, including HCT-116 and HT-29 cells [ 81 ]. In another study, immunoliposomes, coated with antibodies to Frizzled 10 protein, were synthesized and loaded with 5-FU.…”

Section: Extracellular Vesicles: a Novel Drug Delivery Platformmentioning

confidence: 99%

The application of extracellular vesicles in colorectal cancer metastasis and drug resistance: recent advances and trends

et al. 2023

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Colorectal cancer (CRC) has high incidence and mortality rates and is one of the most common cancers of the digestive tract worldwide. Metastasis and drug resistance are the main causes of cancer treatment failure. Studies have recently suggested extracellular vesicles (EVs) as a novel mechanism for intercellular communication. They are vesicular particles, which are secreted and released into biological fluids, such as blood, urine, milk, etc., by a variety of cells and carry numerous biologically active molecules, including proteins, nucleic acids, lipids, metabolites, etc. EVs play a crucial part in the metastasis and drug resistance of CRC by delivering cargo to recipient cells and modulating their behavior. An in-depth exploration of EVs might facilitate a comprehensive understanding of the biological behavior of CRC metastasis and drug resistance, which might provide a basis for developing therapeutic strategies. Therefore, considering the specific biological properties of EVs, researchers have attempted to explore their potential as next-generation delivery systems. On the other hand, EVs have also been demonstrated as biomarkers for the prediction, diagnosis, and presumed prognosis of CRC. This review focuses on the role of EVs in regulating the metastasis and chemoresistance of CRC. Moreover, the clinical applications of EVs are also discussed. Graphical Abstract

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Evaluation of CTB-sLip for Targeting Lung Metastasis of Colorectal Cancer

Cited by 5 publications

References 27 publications

Effects of Surface IR783 Density on the In Vivo Behavior and Imaging Performance of Liposomes

Effects of Surface IR783 Density on the In Vivo Behavior and Imaging Performance of Liposomes

AB Toxins as High-Affinity Ligands for Cell Targeting in Cancer Therapy

The application of extracellular vesicles in colorectal cancer metastasis and drug resistance: recent advances and trends

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