Evaluation of Cultivar Differences in Preharvest Sprouting of Common Buckwheat (<i>Fagopyrum esculentum</i>Moench)

Hara, Takahiro; Tetsuka, Takahisa; Matsui, Kenichi; Ikoma, Hiroki; Sugimoto, Akira

doi:10.1626/pps.11.82

Cited by 7 publications

(5 citation statements)

References 29 publications

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“…Rain after grain maturity but before harvest sometimes induces preharvest sprouting (Choi et al, 1992;Morishita and Tetsuka, 2001;Hara et al, 2007Hara et al, , 2008. Preharvest sprouting in buckwheat decreases the pasting viscosity of buckwheat fl our and buckwheat -wheat composite fl our (Hara et al, 2007).…”

mentioning

confidence: 99%

Effects of Sprouting on Texture of Cooked Buckwheat (Fagopyrum esculentumMoench) Noodles

Hara

Sasaki

Tetsuka

et al. 2009

Plant Production Science

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confidence: 99%

Effects of Sprouting on Texture of Cooked Buckwheat (Fagopyrum esculentumMoench) Noodles

Hara

Sasaki

Tetsuka

et al. 2009

Plant Production Science

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“…QTL analysis and association analysis suggested that the PHS tolerance in KY29 is controlled by dominant alleles at qHS6-KY29 and partially dominant alleles at qHS1-KY29 , fitting our expectation [ 8 ]. As KY29 was developed from a cross between ‘Kanoya-Zairai’ and ‘Hitachiakisoba’, its PHS tolerance alleles might originate from ‘Kanoya-Zairai’, a Japanese landrace with high tolerance [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…We used three PHS-tolerant cultivars/breeding lines: ‘Kyukei 29’ (KY29), ‘Kyukei 28’ (KY28), and ‘NARO-FE-1’ (NF1); those were developed by our research group. KY29 and KY28 had significantly higher PHS tolerance than many leading cultivars in Japan and KSC7, and the evaluation is stable among them [ 5 , 8 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

et al. 2021

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Background Common buckwheat (2n = 2x = 16) is an outcrossing pseudocereal whose seeds contain abundant nutrients and potential antioxidants. As these beneficial compounds are damaged by preharvest sprouting (PHS) and PHS is likely to increase with global warming, it is important to find efficient ways to develop new PHS-tolerant lines. However, genetic loci and selection markers associated with PHS in buckwheat have not been reported. Results By next-generation sequencing (NGS) of whole-genome of parental lines, we developed a genome-wide set of 300 markers. By NGS- based bulked segregant analysis (NGS-BSA), we developed 100 markers linked to PHS tolerance. To confirm the effectiveness of marker development from NGS-BSA data, we developed 100 markers linked to the self-compatibility (SC) trait from previous NGS-BSA data. Using these markers, we developed genetic maps with AmpliSeq technology, which can quickly detect polymorphisms by amplicon-based multiplex targeted NGS, and performed quantitative trait locus (QTL) analysis for PHS tolerance in combination with NGS-BSA. QTL analysis detected two major and two minor QTLs for PHS tolerance in a segregating population developed from a cross between the PHS-tolerant ‘Kyukei 29’ and the self-compatible susceptible ‘Kyukei SC7’. We found different major and minor QTLs in other segregating populations developed from the PHS-tolerant lines ‘Kyukei 28’ and ‘NARO-FE-1’. Candidate markers linked to PHS developed by NGS-BSA were located near these QTL regions. We also investigated the effectiveness of markers linked to these QTLs for selection of PHS-tolerant lines among other segregating populations. Conclusions We efficiently developed genetic maps using a method combined with AmpliSeq technology and NGS-BSA, and detected QTLs associated with preharvest sprouting tolerance in common buckwheat. This is the first report to identify QTLs for PHS tolerance in buckwheat. Our marker development system will accelerate genetic research and breeding in common buckwheat.

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“…In buckwheat, the degree of loss of primary dormancy mainly depends on the storage period and temperature, and a long storage period and high storage temperature are required to lose primary dormancy [ 7 ]. Varietal differences in germination rates have also been reported [ 8 , 9 , 10 ]. Hara et al (2008) [ 7 ] reported that cultivar differences in buckwheat pre-harvest sprouting were correlated with seed dormancy based on a petri dish experiment.…”

Section: Seed Dormancy and Germination In Buckwheatmentioning

confidence: 99%

Breeding of Buckwheat for Usage of Sprout and Pre-Harvest Sprouting Resistance

Suzuki

Hara

Katsu

2021

Plants

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Buckwheat is recognized as an important traditional crop and supports local economies in several regions around the world. Buckwheat is used, for example, as a cereal grain, noodle and bread. In addition, buckwheat is also used as a sprout or a young seedling. For these foods, sprouting is an important characteristic that affects food quality. For foods made from buckwheat flour, pre-harvest sprouting may decrease yield, which also leads to the deterioration of noodle quality. Breeding buckwheat that is resistant to pre-harvest sprouting is therefore required. Germination and subsequent growth are also important characteristics of the quality of sprouts. Although buckwheat sprouts are the focus because they contain many functional compounds, such as rutin, several problems have been noted, such as thin hypocotyls and husks remaining on sprouts. To date, several new varieties have been developed to resolve these quality issues. In this review, we summarize and introduce research on the breeding of buckwheat related to quality, sprouting and subsequent sprout growth.

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Evaluation of Cultivar Differences in Preharvest Sprouting of Common Buckwheat (Fagopyrum esculentumMoench)

Cited by 7 publications

References 29 publications

Effects of Sprouting on Texture of Cooked Buckwheat (Fagopyrum esculentumMoench) Noodles

Effects of Sprouting on Texture of Cooked Buckwheat (Fagopyrum esculentumMoench) Noodles

Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

Breeding of Buckwheat for Usage of Sprout and Pre-Harvest Sprouting Resistance

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