Evaluation of culture media for enumeration of Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium animalis in the presence of Lactobacillus delbrueckii subsp bulgaricus and Streptococcus thermophilus

Evaluation of culture media for selective enumeration of Bifidobacterium spp. in combination with different strains of Streptococcus thermophilus isolated from commercial yogurt starter cultures

“…Similar results reported by Lima et al (2009) confirm the good growth capacity of bifidobacteria in these media.…”

“…However, here the concentration of bile salts was reduced to maintain the high rate of recovery of the bifidobacteria strains analyzed. In contrast, Lima et al (2009) found that the concentration of bile salts did not need to be reduced when the strain B. animalis Bb12 was used. Thus, for the preparation of B-MRS medium a preliminary study to determine the adequate concentration of bile salts must be performed for each probiotic strain Several studies indicate that LP-MRS medium is selective for bifidobacteria in the presence of the starter culture.…”

“…MRS agar was also used for counting the total lactic acid bacteria (S. thermophilus and L. delbrueckii subsp. bulgaricus) in the mixed cultures (Lima et al, 2009). …”

“…A wide variety of culture media have been proposed in the literature for the selective enumeration of bifidobacteria in the presence of yogurt bacterial components (Rybka and Kailasapathy, 1996;Vinderola and Reinheimer, 1999;Talwalkar and Kailasapathy, 2004;Casteele et al, 2006;Lima et al, 2009). Some studies have used different commercial starter cultures, but the identification of the strains that comprise these cultures and their effects on the selection of the selective culture medium have not been established.…”

Functional Properties (Acid and Bile Tolerance) and Antibiotic Susceptibility of Lactic Acid Bacteria Isolated from Newborn Calves for the Design of a Probiotic Product

“…The diffusion of the antibiotic can be modified by the composition or pH of the media and is of major importance to obtain reproducible results [24]. In the case of LABs, different media were used [42][43][44]. In this work, the LSM media proposed by Klare et al [25], and studied by others authors was included [16,42], supporting the growth of most of the strains, with the exception of L. mucosae strains.…”