Evaluation of Curcumin Capped Copper Nanoparticles as Possible Inhibitors of Human Breast Cancer Cells and Angiogenesis: a Comparative Study with Native Curcumin

Abstract

Abstract. Synthesis of metal nanoparticles for improving therapeutic index and drug delivery is coming up as an attractive strategy in the mainstream of cancer therapeutic research. In the present study, curcumincapped copper nanoparticles (CU-NPs) were evaluated as possible inhibitors of in vivo angiogenesis, proangiogenic cytokines involved in promoting tumor angiogenesis along with inhibition of cell proliferation and migration of breast cancer cell line MDA-MB-231. The antiangiogenic potential was assessed using in vivo chorioallantoic membrane (CAM) model. 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)-based cytotoxicity assay was used to assess the effect of CU-NPs against proliferation of breast cancer cell line. The wound healing migration assay was used to evaluate the effects of CU-NPs on the migration ability of breast cancer cell line. Native curcumin (CU) was used as a reference compound for comparison purpose. The result of the present investigation indicates that CU-NPs could not demonstrate impressive antiangiogenic or anticancer activities significantly as compared to native CU. The possible mechanisms of experimental outcomes are discussed in the light of the methods of nanoparticle synthesis in concert with the current state of the art literature.

Prosopis juliflora (Sw.), DC induces apoptosis and cell cycle arrest in triple negative breast cancer cells: in vitro and in vivo investigations

“…The chorioallantoic membrane (CAM) assay was performed as described in our previous reports [ 42 ]. The anti-angiogenic potential of PJLME was determined using formula, 1−T/C, where T indicates the no.…”

Biological properties of metal complexes of curcumin

“…Angiogenesis plays an important role in cancer development and progression ; therefore, targeting angiogenesis could be a promising strategy for cancer therapy . It was demonstrated that curcumin‐capped copper nanoparticles have inhibitory effects on human breast cancer cell line MDA‐MB‐231 and angiogenesis . One study reported that curcumin complexation with copper considerably enhanced the antitumor effects of curcumin and its derivatives .…”

Curcumin–Copper Complex Nanoparticles for the Management of Triple-Negative Breast Cancer

“…Previous studies have shown copper alone to be cytotoxic against cancer cells, albeit at a concentration above 100 µM [ 24 ], although copper nanoparticles coated with albumin displayed cytotoxicity in TNBC cells above 50 µM [ 25 ]. Furthermore, a previous study using curcumin-capped copper nanoparticles failed to demonstrate any cytotoxicity against TNBC cell lines [ 26 ]. However, more recently, copper(II) phosphorus dendrimers demonstrated cytotoxicity against cancer cells [ 27 ].…”