Evaluation of Curcumin Therapeutic Effects on Histological Subtypes of Canine Mammary Gland Tumours

Turna, Özge; Baykal, Aslihan; Kucukkara, Elif Sozen; Özkan, Asuman Deveci; Eskiler, Gamze Güney; Yıldırım, Funda

doi:10.1080/01635581.2022.2032216

Cited by 2 publications

(4 citation statements)

References 39 publications

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“…In these conditions, several curcumin analogues have shown potent, submicromolar cytotoxicity, with negligible systemic toxicity in murine models 21,22 . To date, limited studies have occurred in canine models, however, a recent study demonstrated curcumin to have differing apoptotic effects in two cases of canine mammary gland cancer 23 . This difference in effect was hypothesised to be related with progression and invasiveness of the tumour.…”

Section: Introductionmentioning

confidence: 99%

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The second‐generation curcumin analogue RL71 elicits G2/M cell cycle arrest and apoptosis in canine osteosarcoma cells

Kelly

Thamm

Rosengren

2023

Vet Comparative Oncology

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Canine osteosarcoma is an aggressive cancer, comprising 85% of canine bone neoplasms. Current treatment practices of surgery and chemotherapy increase 1‐year survival by only 45%. The curcumin analogue RL71, has demonstrated potent in vitro and in vivo efficacy in several models of human breast cancer through increased apoptosis and cell cycle arrest. Thus, the present study aimed to investigate efficacy of curcumin analogues in two canine osteosarcoma cell lines. Osteosarcoma cell viability was assessed using the sulforhodamine B assay and mechanisms of action were determined by analysing the levels of cell cycle and apoptotic regulatory proteins via Western blotting. Further evidence was obtained using flow cytometry to detect cell cycle distribution and the number of apoptotic cells. RL71 was the most potent curcumin analogue with EC50 values of 0.64 ± 0.04 and 0.38 ± 0.009 μM (n = 3) in D‐17 (commercial) and Gracie canine osteosarcoma cells, respectively. RL71 significantly increased the ratio of cleaved‐caspase 3 to pro‐caspase 3 and the level of apoptotic cells at the 2× and 5× EC50 concentration (p < 0.001, n = 3). Furthermore, at the same concentration, RL71 significantly increased the number of cells in the G2/M phase. In conclusion, RL71 has potent cytotoxic activity in canine osteosarcoma cells triggering G2/M arrest and apoptosis at concentrations achievable in vivo. Future research should further investigate molecular mechanisms for these changes in other canine osteosarcoma cell lines prior to in vivo investigation.

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Section: Introductionmentioning

confidence: 99%

“…21,22 To date, limited studies have occurred in canine models, however, a recent study demonstrated curcumin to have differing apoptotic effects in two cases of canine mammary gland cancer. 23 This difference in effect was hypothesised to be related with progression and invasiveness of the tumour.…”

mentioning

confidence: 99%

The second‐generation curcumin analogue RL71 elicits G2/M cell cycle arrest and apoptosis in canine osteosarcoma cells

Kelly

Thamm

Rosengren

2023

Vet Comparative Oncology

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“…[14] Studies have shown that CUR may have a protective effect in the treatment process of breast cancer. [13,15,16] In our literature review, we found a paucity of studies on the occurrence of cardiac damage caused by ABE administration. In this study, we aimed to investigate whether the use of ABE can cause cardiotoxicity and whether CUR in combination with ABE has a suppressive effect on ABE-induced cardiotoxicity.…”

Section: Introductionmentioning

confidence: 99%

“…[ 14 ] Studies have shown that CUR may have a protective effect in the treatment process of breast cancer. [ 13,15,16 ]…”

Section: Introductionmentioning

confidence: 99%

The protective effect of curcumin on cardiac markers and fibrosis in abemaciclib‐induced cardiac damage in rats

Huyut

Uçar

Yıldızhan

et al. 2022

J Biochem & Molecular Tox

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Abemaciclib (ABE) is a cyclin‐dependent kinase inhibitor used in combination with an antiestrogen in the treatment of breast cancer. In addition to the important therapeutic properties of this drug, its side effects are not fully known. In this study, we aimed to investigate the protective effect of curcumin (CUR) on cardiac damage caused by ABE administration. Forty rats were equally divided into control, dimethyl sulfoxide (150 µL), CUR (30 mg/kg/day), ABE (26 mg/kg/day), and ABE + CUR (26 mg/kg/day ABE and 30mg/kg/day CUR) groups (n = 8). Injections were administered daily for 28 days. Troponin‐I, total cholesterol, and creatine kinase myocardial band (CK‐MB) levels and cardiac fibrosis were higher in the ABE group than in the control group (p < 0.05), and were lower in the ABE + CUR group than in the ABE group (p < 0.05). The results showed that ABE administration can cause cardiac damage and increase cardiac fibrosis. However, they showed that coadministration of CUR with ABE could suppress increases in CK‐MB, troponin‐I, and total cholesterol levels and also cardiac fibrosis associated with cardiac damage. Therefore, we can infer that the subsequent administration of CUR ABE treatment can be used as a therapeutic strategy for preventing cardiac damage.

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Evaluation of Curcumin Therapeutic Effects on Histological Subtypes of Canine Mammary Gland Tumours

Cited by 2 publications

References 39 publications

The second‐generation curcumin analogue RL71 elicits G2/M cell cycle arrest and apoptosis in canine osteosarcoma cells

The second‐generation curcumin analogue RL71 elicits G2/M cell cycle arrest and apoptosis in canine osteosarcoma cells

The protective effect of curcumin on cardiac markers and fibrosis in abemaciclib‐induced cardiac damage in rats

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