The aim of this in vitro study was to investigate the safety of using blue diode laser (445 nm) for tooth bleaching with regard to intrapulpal temperature increase operating at different average power and time settings. Fifty human mandibular incisors (n = 10) were used for evaluating temperature rise inside the pulp chamber and in the bleaching gel during laser-assisted tooth bleaching. The change in temperature was recorded using K thermocouples for the five experimental groups (without laser, 0.5, 1, 1.5 and 2 W) at each point of time (0, 10, 20, 30, 40, 50 and 60 s). As the average power of the diode laser increases, the temperature inside the pulp chamber also increases and that of the bleaching gel was significantly higher in all the experimental groups (p < 0.05). However, the intrapulpal temperature rise was below the threshold for irreversible thermal damage of the pulp (5.6 °C). Average power of a diode laser (445 nm) ranging between 0.5–2 W and irradiation time between 10–60 s should be considered safe regarding the pulp health when a red-colored bleaching gel is used. Clinical studies should confirm the safety and effectiveness of such tooth bleaching treatments. The outcomes of the present study could be a useful guide for dental clinicians, who utilize diode lasers (445 nm) for in-office tooth bleaching treatments in order to select appropriate power parameters and duration of laser irradiation without jeopardizing the safety of the pulp.