Evaluation of cytotoxic and apoptotic effects of the extracts and phenolic compounds of Astragalus globosus Vahl and Astragalus breviflorus DC

Kalaycı, Benan; Şimşek Özek, Nihal; Aysin, Ferhunde; Özbek, Hilal; Kazaz, Cavit; Önal, Mehmet; Güvenalp, Zühal

doi:10.1016/j.jsps.2023.06.015

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…Indeed, our research strongly confirms that within the Astragalus genus, flavonoids hold a prominent position as the most abundant category of secondary metabolites. Moreover, the phenolic compounds mentioned earlier in our research were discovered in the methanol extract of A. schizopterus [ 22 ]. Similarly, the key phenolic compounds, including kaempferol, quercetin, rutin, and rosmarinic acid, were identified in separate Astragalus species using two HPLC techniques [ 22 ].…”

Section: Discussionmentioning

confidence: 80%

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Anti-Cholinergic Effects of the Phenolic Extract from the Astragalus crenatus Plant: A Computational and Network Pharmacology Study

Lekmine,

Benslama,

Tahraoui

et al. 2024

Pharmaceuticals

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Investigations into cholinesterase inhibition have received attention from researchers in recent years for the treatment of Alzheimer’s disease. Cholinesterase enzymes, namely, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), hold pivotal significance in Alzheimer’s disease (AD) treatment. In this study, we utilized the ethanolic extract of Astragalus crenatus followed by liquid chromatography–electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) to separate and identify at least 21 compounds in the extract. Rosmarinic acid exhibited the highest concentration (96.675 ± 1.3 mg/g extract), succeeded by hesperidin (79.613 ± 1.2 mg/g extract), hesperetin (75.102 ± 1.4 mg/g extract), rutin (68.156 ± 1.6 mg/g extract), chlorogenic acid (67.645 ± 1.5 mg/g extract), fisetin (66.647 ± 2.3 mg/g extract), and hyperoside (63.173 ± 1.5 mg/g extract). A. crenatus extract efficiently inhibited both AChE and BChE activities in a dosage-dependent manner. Molecular docking was employed to scrutinize the anticholinesterase mechanisms of the identified phytocompounds. Notably, a network pharmacology analysis was executed for the most efficacious compound. Based on binding energies, hesperidin emerged as the most potent inhibitor against both AChE and BChE, exhibiting scores of −10.5 Kcal/mol and −9.8 Kcal/mol, respectively. Due to its dual inhibition of AChE and BChE activities, hesperidin from Astragalus crenatus holds promise for the development of novel therapeutics aimed at neurological disorders, particularly AD.

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Section: Discussionmentioning

confidence: 80%

“…Moreover, the phenolic compounds mentioned earlier in our research were discovered in the methanol extract of A. schizopterus [ 22 ]. Similarly, the key phenolic compounds, including kaempferol, quercetin, rutin, and rosmarinic acid, were identified in separate Astragalus species using two HPLC techniques [ 22 ].…”

Section: Discussionmentioning

confidence: 80%

Anti-Cholinergic Effects of the Phenolic Extract from the Astragalus crenatus Plant: A Computational and Network Pharmacology Study

Lekmine,

Benslama,

Tahraoui

et al. 2024

Pharmaceuticals

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“…Many researchers have found that extracts from different species of the Astragalus genus exhibit varying degrees of cytotoxicity against Artemia salina . These studies typically involve preparing extracts from different plant parts (such as roots, leaves, or seeds) using various extraction methods (such as solvent extraction or maceration) [ 57 ]. The cytotoxic activity of these extracts is then assessed by exposing Artemia salina nauplii to different concentrations of the extract and monitoring their mortality rate over a specific period.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Investigation of Astragalus arpilobus subsp. hauarensis: LC-MS/MS Chemical Profiling, In Vitro Evaluation of Antioxidant, Anti-Inflammatory Properties, Cytotoxicity, and In Silico Analysis against COX-2

Lekmine,

Benslama,

Kadi

et al. 2024

Antioxidants

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The search results offer comprehensive insights into the phenolic compounds, antioxidant, anti-inflammatory, cytotoxic effects, LC-MS/MS analysis, molecular docking, and MD simulation of the identified phenolic compounds in the Astragalus arpilobus subsp. hauarensis extract (AAH). The analysis revealed substantial levels of total phenolic content (TPC), with a measured value of 191 ± 0.03 mg GAE/g DM. This high TPC was primarily attributed to two key phenolic compounds: total flavonoid content (TFC) and total tannin content (TTC), quantified at 80.82 ± 0.02 mg QE/g DM and 51.91 ± 0.01 mg CE/g DM, respectively. LC-MS/MS analysis identified 28 phenolic compounds, with gallic acid, protocatechuic acid, catechin, and others. In the DPPH scavenging assay, the IC50 value for the extract was determined to be 19.44 ± 0.04 μg/mL, comparable to standard antioxidants like BHA, BHT, ascorbic acid, and α-tocopherol. Regarding anti-inflammatory activity, the extract demonstrated a notably lower IC50 value compared to both diclofenac and ketoprofen, with values of 35.73 µg/mL, 63.78 µg/mL, and 164.79 µg/mL, respectively. Cytotoxicity analysis revealed significant cytotoxicity of the A. arpilobus extract, with an LC50 value of 28.84 µg/mL, which exceeded that of potassium dichromate (15.73 µg/mL), indicating its potential as a safer alternative for various applications. Molecular docking studies have highlighted chrysin as a promising COX-2 inhibitor, with favorable binding energies and interactions. Molecular dynamic simulations further support chrysin’s potential, showing stable interactions with COX-2, comparable to the reference ligand S58. Overall, the study underscores the pharmacological potential of A. arpilobus extract, particularly chrysin, as a source of bioactive compounds with antioxidant and anti-inflammatory properties. Further research is warranted to elucidate the therapeutic mechanisms and clinical implications of these natural compounds.

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“…New activities were sought for this widely distributed flavonoid in nature. Diosmetin has been studied for its anticancer [7][8][9] and anti-inflammatory [10][11][12] properties. Moreover, its potential use in diseases associated with acetylcholinesterase (AchE) and butyrylcholinesterase (BuChE) inhibition has been investigated [13,14].…”

Section: Introductionmentioning

confidence: 99%

Can We Improve Diosmetin Activity? The State-of-the-Art and Promising Research Directions

Wujec,

Feldo

2023

Molecules

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Diosmetin is a natural substance widely distributed in nature, with documented multidirectional biological effects. The wide spectrum of biological activity of diosmetin gives hope that derivatives of this flavonoid may also be used as drugs or dietary supplements used in many diseases. Modification of the structure may, on the one hand, lead to an increase in biological potency, new biological activity, or an increase in solubility and thus bioavailability. This is an important direction of research because the use of pure diosmetin is limited due to its low bioavailability. This work is an attempt to collect information on the possibility of modifying the structure of diosmetin and its impact on biological activity.

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Evaluation of cytotoxic and apoptotic effects of the extracts and phenolic compounds of Astragalus globosus Vahl and Astragalus breviflorus DC

Cited by 5 publications

References 36 publications

Anti-Cholinergic Effects of the Phenolic Extract from the Astragalus crenatus Plant: A Computational and Network Pharmacology Study

Anti-Cholinergic Effects of the Phenolic Extract from the Astragalus crenatus Plant: A Computational and Network Pharmacology Study

Preliminary Investigation of Astragalus arpilobus subsp. hauarensis: LC-MS/MS Chemical Profiling, In Vitro Evaluation of Antioxidant, Anti-Inflammatory Properties, Cytotoxicity, and In Silico Analysis against COX-2

Can We Improve Diosmetin Activity? The State-of-the-Art and Promising Research Directions

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