Evaluation of cytotoxicity and genotoxicity of Hancornia speciosa latex in Allium cepa root model

Ribeiro, Thuanne Pires; Sousa, T. R. de; Arruda, Andrelisse; Peixoto, Nei; Gonçalves, Pablo José; Almeida, Luciane Madureira

doi:10.1590/1519-6984.20114

Cited by 42 publications

(27 citation statements)

References 18 publications

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“…The fruit extract and the trunk latex exhibited anti-inflammatory activity in animal models [ 36 , 37 ]. Additionally, the latex showed significant angiogenic activity and a potential osteogenic effect, and had no cytotoxic or genotoxic effects on life systems [ 19 , 38 , 39 ]. Chemical analysis of H. speciosa latex resulted in the identification of naringenin-7- O -glucoside, chlorogenic acid, catechin, and procyanidin [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Bastos¹,

Dias²,

Nascimento³

et al. 2017

Molecules

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Apocynaceae is a botanical family distributed mainly in tropical and subtropical regions of the world. In Brazil, they comprise about 90 genera and 850 species, inhabiting various types of vegetation. Within this large botanical family, the genus Hancornia is considered monotypic, with its only species Hancornia speciosa Gomes. Antihypertensive, antidiabetic, and antiviral activities are described for this species. Despite having been the target of some studies, knowledge of its chemical composition is still limited. In this study, the phenolics of H. speciosa leaves were analyzed using ultra-high performance liquid chromatography (UHPLC) coupled to Orbitrap high-resolution mass spectrometry (HRMS). As a result, 14 compounds were identified viz. protocatechuic acid, catechin, and quercetin, and another 14 were putatively identified viz. B- and C-type procyanidins, while just one compound remained unknown. From the identified compounds, 17 are reported for the first time viz. coumaroylquinic acid isomers and eriodyctiol. The results show that Hancornia speciosa can serve as source of valuable phenolics.

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Section: Discussionmentioning

confidence: 99%

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Bastos¹,

Dias²,

Nascimento³

et al. 2017

Molecules

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“…The Allium cepa assay has been widely used for evaluation of cytotoxic and genotoxic activity of various compounds (RIBEIRO et al, 2016;DEL CAMPO et al, 2005;BARBÉRIO et al, 2009;ROA et al, 2012;FRESCURA et al, 2013), including medicinal herbs (BAGATINI et al, 2007). It has been validated by an international collaborative study by the United Nations Environmental Program (UNEP), World Health Organization (WHO) and US Environmental Protection Agency (USEPA) as an efficient test for genetic monitoring (BADMUS et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Jatropha curcas L. latex in Allium cepa test

Ciappina¹,

Ferreira²,

Pereira³

et al. 2017

Biosci. J.

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ABSTRACT:The latex obtained from Jatropha curcas (physic nut) is used in traditional medicine to treat a variety of disturbs, including burns, hemorrhoids, ringworm and ulcers. Phytochemical analyses have shown that J. curcas latex contains natural compounds with therapeutic potential. In this study, the toxicity, cytotoxicity and genotoxicity effects of J. curcas latex on the root cells of Allium cepa were examined. Onion seeds and bulbs were exposed to seven different concentrations of latex and then the roots were submitted to macro and microscopic analyses. Water and sodium azide were used as negative and positive controls, respectively. The analysis of root growth showed that J. curcas crude latex or 50% diluted is highly toxic. Cytogenetic results showed that the mitotic index of the onion roots submitted to latex treatment decreased significantly compared to the negative control, which suggests that the latex is cytotoxic. High incidence of chromosome aberrations in the cells treated with J. curcas latex was observed too, indicating that the latex also presents genotoxic effect. The analyses presented in this report suggest the toxic, cytotoxic and genotoxic effects of J. curcas latex. Then, the indiscriminate use of J. curcas latex in folk medicine could bring risk to human health.

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“…Here we selected two different methodologies to evaluate the V. polyanthes toxicological potential: Allium cepa and Artemia salina assays. Those methods are very useful for toxicity and cytogenotoxicity screening and they are simple, inexpensive and minimum laboratory facilities are required for its performance (Ribeiro et al, 2016;Neves et al, 2014;Leme and Marin-Morales, 2009). Moreover, the results obtained using those in vivo models show a high degree of conformity with the results obtained from mammalian assays (Freires et al, 2017;Fedel-Miyasato et al, 2014;Leme and Marin-Morales, 2009).…”

mentioning

confidence: 81%

Cytotoxic effect of Vernonanthura polyanthes leaves aqueous extracts

et al. 2021

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Vernonanthura polyanthes, popularly known as assa-peixe, is a medicinal plant that has been widely used by Brazilian Cerrado population for treatment of diseases without a detailed evaluation of their effectiveness, toxicity, and proper dosage. Thus, more studies investigating the safety of V. polyanthes aqueous extract before the use are needed. The purpose of this study was to evaluate the toxicity, cytotoxicity and genotoxicity of V. polyanthes leaves aqueous extract using the Artemia salina and Allium cepa assays. For the A. salina assay, three groups of 10 larvae were exposed to V. polyanthes leaves aqueous extract at the concentrations of 5, 10, 20, 40, and 80 mg/ml. For the A. cepa assay, 5 onion bulbs were exposed to V. polyanthes leaves aqueous extract at 10, 20, and 40 mg/ml, and then submitted to macroscopic and microscopic analysis. As result it was identified a toxicity and cytotoxicity of V. polyanthes dependent on the extract concentration. The A. salina assay suggests that the concentration of 24 mg/ml of the V. polyanthes extract is able to kill 50% of naupllis; while the A. cepa assay suggests that V. polyanthes leaves aqueous extract is toxic at concentrations higher than 20 mg/ml; however the cytotoxic effect in A. cepa root cells was observed at 40 mg/ml of the extract. It is important to say that the V. polyanthes leaves aqueous extract concentration commonly used in popular medicine is 20 mg/ml. Thus, the popular concentration used is very close to toxicity limit in A. salina model (24 mg/ml) and is the concentration which showed toxic effect in A. cepa root cells (20 mg/ml). No genotoxic activity of V. polyantes leaves aqueous extract was observed in the conditions used in this study. Because of the antiproliferative action and no genotoxic activity, V. polyanthes leaves aqueous extract may present compounds with potential use for human medicine. However more detailed studies need to be performed to confirm this potential.

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Evaluation of cytotoxicity and genotoxicity of Hancornia speciosa latex in Allium cepa root model

Cited by 42 publications

References 18 publications

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Toxicity of Jatropha curcas L. latex in Allium cepa test

Cytotoxic effect of Vernonanthura polyanthes leaves aqueous extracts

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