Evaluation of Cytotoxicity of Silorane and Methacrylate based Dental Composites using Human Gingival Fibroblasts

Madhyastha, Prashanthi S; Naik, Dilip G; Kotian, Ravindra; Padma, Divya; Natarajan, Srikant; Bhat, Kumar Mr

doi:10.7860/jcdr/2015/10269.5366

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…Although we observed a cytotoxic effect of the silorane composite resin, this was not reflected on the MTT assay. Likewise, another study suggested the non-toxic nature of the silorane composite resin in human fibroblasts treated with Filtek P90, which showed only an insignificant decrease in cell proliferation in 24 h and 48 h 12 .…”

Section: Discussionmentioning

confidence: 96%

Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins

et al. 2016

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The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages.Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05).Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05).Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.

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Section: Discussionmentioning

confidence: 96%

Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins

et al. 2016

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“…It should be noted that genotoxic damage usually goes unnoticed, however, it produces a directly or indirectly effect on DNA and could be express as mutations with teratogenic or carcinogenic effect; the result is mostly catastrophic, since they put the organism's life at risk [3,[11][12][13][14][15]24]. The increase of oxidative stress and genotoxic damage has been described in dental patients as a result of exposure to different materials and procedures [10,17,25,26].…”

Section: Discussionmentioning

confidence: 99%

“…For their part, dental surgeons and technicians have chronic exposure to agents that increase oxidative stress and could be danger to genetic material [2]. This materials can be ultraviolet light [2], x-rays, residual monomers [3], root canal sealer [4,5], resin-based materials [6,7], zinc oxide-eugenol cement [2], hydrogen peroxide and beryllium [8].They are also exposed to materials with cytotoxic effects such as silver welding, copper, zinc, cadmium, nickel, triethylene glycol dimethacrylate (TEGDMA) and whitening gels [5][6][7]9]. Additional to the everyday innovation of dental materials with more sophisticated structural and therapeutic functions, which increases the need to supervise, foresee and/or control the possible threats [10].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genotoxicity and cytotoxicity amongst in dental surgeons and technicians by micronucleus assay

Daniel¹,

Eugenia²,

Jafet³

et al. 2019

Dent Oral Maxillofac Res

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Introduction: Dental surgeons and technicians are continuously exposed to agents could be affect the genetic material and induce mutations. The aim of this study was to evaluate the genotoxic and cytotoxic occupational risk of dental surgeons and technicians through the micronucleated cells (MNC) and nuclear abnormalities (NA) assay in oral mucosa.Methods: Case-control study. We have collected a buccal mucosa from dental surgeons, dental technicians and healthy individuals (matched by BMI, age and gender). The smears were fixed (ethanol 80%/48 h), stained (orange acridine) and analyzed (microscope, 100×). The frequency of MNC and NA (binucleated cells [BNC], lobulated nucleus [LN], condensed chromatins [CC], karyorrhexis [KR], pyknosis (PN) and karyolysis [KL] were counted in 2,000 cells per participant.Results: 90 samples were collected (26 surgeons, 19 technicians and 45 controls). Compared with controls, exception of PN, in surgeons was higher frequency and positive association of MNC and all NA (p<0.05). Technicians were higher frequency of MNC, BNC, CC, KR and CD (p<0.05). Conclusion:The dental surgeons and technicians have genotoxic and cytotoxic damage, demonstrated by MNC and NA, which are related to an increase risk of cancer, chronic degenerative diseases and premature aging. It is necessary to improve biosecurity actions and implement routine genotoxic risk detection methods.

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“…However, it has been showed that released TEGDMA was generally higher compared to other monomers. [39] On the other hand, silorane-based restorative composites have shown comparable [40] or better [41] cytotoxic characteristics to clinically successful dimethacrylate composites. In the present study, SR was biocompatible except for the 7-day extracts after 72 h incubation.…”

Section: Discussionmentioning

confidence: 99%

A comparative study on monomer elution and cytotoxicity of different adhesive restoration materials

Toz

Kiremitçi

Çakmak

et al. 2016

Journal of Adhesion Science and Technology

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This study evaluated monomer release and cytotoxicity of different adhesive restoration materials used for dental restorations. The extracts (1, 2, and 7 days) of three types of adhesive dental restoration materials, [Quixfill (QF), Silorane Restorative (SR), and Ketac N 100 Restorative (KR)], and the adhesive resins, [XP Bond (XP), Silorane Primer (SP), Ketac N 100 Primer (KP), and Silorane Bond (SB)] were analyzed using high performance liquid chromatography/mass spectrometry (HPLC-MS). The cytotoxicity levels were determined at different time points (24, 48, and 72 h) of cell culture using 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay. All adhesive resin materials showed monomer release at varying amounts with the highest release after 7 days. The lowest amount of release was observed in QF and the highest with KP. Bis-Phenol A (BPA) was not detected in SP and KR that contain bisphenol-A diglycidyl ether dimethacrylate (bis-GMA). Decamethylpenthasiloxane (D5) was not eluted from SR. Except for SR and QF, all other adhesive restoration materials showed different degrees of toxicity along with different monomer release kinetics. The correlation between the monomer release and cytotoxicity of the materials indicated that the cytotoxicity of the materials increased with the monomer release (Spearman's rho correlation coefficient-r). The correlation after 48 h was statistically significant (r = −0.342, p = 0.017).

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Evaluation of Cytotoxicity of Silorane and Methacrylate based Dental Composites using Human Gingival Fibroblasts

Cited by 6 publications

References 26 publications

Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins

Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins

Evaluation of genotoxicity and cytotoxicity amongst in dental surgeons and technicians by micronucleus assay

A comparative study on monomer elution and cytotoxicity of different adhesive restoration materials

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