Evaluation of Cytotoxicity, Total Phenolic Content and Antioxidant Innate Reveal Efficient Medications in Native Lactuca indica

Abstract

Lactuca indica is an edible wild vegetable, which is frequently availed as a folk remedy for its anti-inflammatory, antibacterial, and other treatments in Asia. This study was executed to evaluate the cytotoxicity, antioxidant and phenolic contents of the methanol extracts from different organs of Lactuca indica. The highest amount of phenolics found in the methanol extracts of leaf (35.09 ± 0.15 mg/g), followed by stem (15.44 ± 0.20 mg/g), root (13.50 ± 0.19 mg/g) and flower (12.50 ± 0.39 mg/g) while the highest flavonoid contents also observed in the methanol extracts of leaf (26.90 ± 0.22 mg/g). Methanol extracts of leaf showed a pronounced DPPH radical scavenging activity (90.37 ± 0.15 mg/mL) and the ABTS radical scavenging activity (99.84 ± 0.02 mg/mL) at 10 mg/mL and 20 mg/mL respectively. Using MTT assay, the methanol extracts of leaf showed the highest cytotoxicity (IC 50 113.84 μg/mL) against human breast adenocarcinoma cell (MCF-7). To this end, the results revealed that the phenolic contents were highly correlated with the DPPH and ABTS radical scavenging activity. The findings suggest that it could contribute to the antioxidant properties of different organs of the studied plant. In this regard, the cytotoxicity and antioxidant properties could be helpful for the evaluation of the bioactive properties of Lactuca indica.

In vitro propagation, lactucin quantification, and antibacterial activity of Indian lettuce (Lactuca indica L.)

“…Lactuca indica extracts have been shown to have various therapeutic activities, including antibacterial activity against uropathogenic E. coli (Hou et al 2003;Wang et al 2003;Kim et al 2007Kim et al , 2010Lüthje et al 2011;Park et al 2014). Lactucin in Cichorium intybus showed antibacterial activity against various bacteria (Petrovic et al 2004;Nandagopal and Kumari 2007;Liu et al 2013).…”

“…Traditionally, it is used in a tonic to heal fever, coughs, diabetes, and stomach disorders. The plant has antiinflammatory, antidiarrhea, cytotoxicity, antibacterial, antidiabetic, antioxidant, α-glucosidase-inhibiting, and immune-enhancing activities (Hou et al 2003;Wang et al 2003;Kim et al 2007Kim et al , 2008Kim et al , 2010Harikrishnan et al 2011;Lüthje et al 2011;Park et al 2014;Choi et al 2016;Ha et al 2017;Oliya et al 2018). Like other Lactuca species, its leaves and stems release a milky latex upon wounding, which becomes sticky when exposed to air, helping to defend against herbivorous insects (Agrawal and Konno 2009).…”

ANTIOXIDANT ACTIVITY TEST of SIJUKKOT (Lactuca indica L.) LEAVES EXTRACT LEAF

“…Tanaman sijukkot memiliki banyak khasiat karena mengandung senyawa golongan fenolik dan polifenol seperti flavonoid. [10][11][12][13][14][15] 16 Diidentifikasi delapan senyawa fenolik dalam Lactuca indica. Struktur senyawa dijelaskan berdasarkan bukti spektroskopi sebagai apigenin, luteolin, isoquercitrin, asam klorogenat, asam protocatechuic, asam p-hydroxymethyl benzoic, asam trans-cinnamic, dan asam p-coumaric, Luteolin, isoquercitrin, asam klorogenat, dan asam p-hidroksimetil benzoate.…”