Evaluation of denture base resin after disinfection method using reactive oxygen species (ROS)

Abstract

The effects of certain disinfectants on the stability of a polymethyl methacrylate denture base resin were investigated, including those of a novel disinfection method using reactive oxygen species (ROS). The surface roughness and flexural strength were analyzed to assess the effects of the disinfectants on material properties. The following disinfectants were tested: 5% sodium hypochlorite, 70% alcohol, and ROS. Furthermore, the attachment of Candida albicans to the resin surface was investigated. The disinfection method using sodium hypochlorite significantly increased the surface roughness and decreased flexural strength. The surface roughness and flexural strength of the ROS-treated specimens did not significantly differ from those of the control specimens, and the ROS-treated specimens exhibited diminished Candida attachment. These results demonstrate that the ROS disinfection method preserves acceptable material stability levels in polymethyl methacrylate resins.

Effect of surface roughness on the hydrophobicity of a denture‐base acrylic resin andCandida albicanscolonization

“…In contrast, Odagiri et al . found that disinfection of an autopolymerized denture‐base resin with 5% sodium hypochlorite promoted an increase in roughness (Ra 1.04) and the initial attachment of C. albicans when compared to the control (immersion in water, Ra 0.38) . It should be noted, however, that the increase in C. albicans adherence, although significant, was small (<0.5 log 10 c.f.u./mL).…”

“…As in previous investigations on the adherence and biofilm formation of Candida albicans to denture‐base resins, the arithmetic Ra was chosen to represent the surface roughness in the present study …”

“…albicans adhesion, as well as proliferation on the most commonly‐used denture‐base material, polymethylmethacrylate, conflicting results have been reported. While some studies have shown that, during initial adherence, C. albicans is retained in greater amounts on rough surfaces in comparison to smooth acrylic surfaces, others have observed no significant influence of surface roughness on the initial attachment of C. albicans . It is important to note, however, that although in these studies the substrates were prepared to produce different surface morphologies, only a few investigated the adherence of C. albicans to denture‐base resins over wider range of roughness .…”

Influence of ozone and paracetic acid disinfection on adhesion of resilient liners to acrylic resin

“…These properties make ozone an important therapeutic agent in infectious and inflammatory diseases 8. Furthermore, ozone decomposes rapidly to O 2 and OH radicals in water solutions 910…”

Photosensitization of in vitro biofilms formed on denture base resin

“…Immersion in 70% ethanol for 15 minutes caused qualitative changes in the acrylic resin surface, as observed by scanning electron microscope. 33 The search for a simple alternative method of disinfection or sterilization of acrylic resin devices is of interest to clinical dentistry because of problems of cross-infection between the laboratory and dental office and the use of nonsterile immediate dentures and surgical guides. 31 In addition, a wide range of potentially pathogenic microorganisms has been found in previously worn dentures.…”