Evaluation of dermal tissue regeneration using resveratrol loaded fibrous matrix in a preclinical mouse model of full-thickness ischemic wound

Lakshmanan, Rajesh; Campbell, Jacob; Ukani, Gopi; Beringhs, André O’Reilly; Selvaraju, Vaithinathan; Thirunavukkarasu, Mahesh; Lü, Xiuling; Palesty, J. Alexander; Maulik, Nilanjana

doi:10.1016/j.ijpharm.2019.01.001

Cited by 30 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Application of wound dressings containing resveratrol to full-thickness skin wounds induced marked reductions in wound areas in comparison with the controls [122]. Similar acceleration in wound closure occurs following the placement of scaffolds containing resveratrol over the wound [123]. is evidence indicates that topical resveratrol accelerates wound healing in normal mice.…”

Section: Anti-inflammationmentioning

confidence: 77%

“…Rats were fed with resveratrol at a daily dose of 0.5 mg/kg body weight 7 days prior to operation and continued throughout the whole experiment period ↑Collagen deposition ↑Neovascularization ↑Fibroblast maturation ND [120] Following induction of full-thickness skin wound, wound was treated topically with 225 μL of 50 μM once daily for 17 days ↑Epithelialization ↓Wound size ↑Collagen deposition ↑Vascularization ↑AMPK pathway and SIRT1 [121] Mice Immediately after wound, a wound dressing containing 0.04% resveratrol was applied to full-thickness skin wound for 10 days ↓Wound size ↑Collagen fibers ↓Inflammation ND [122] Placing scaffolds containing 5% resveratrol on the wound for 7 days ↓Wound size ↑Expression of thioredoxin-1, heme oxygenase-1, and VEGF [123] Evidence-Based Complementary and Alternative Medicine structures for epidermal permeability barrier, requires transglutaminase [127]. Treatment of keratinocytes with resveratrol induced a dose-dependent increase in transglutaminase activity, while simultaneously inhibiting DNA synthesis, with a half-maximal concentration of 35 μM.…”

Section: Ratsmentioning

confidence: 99%

“…SIRT1-mediated benefits of resveratrol in diabetic wound healing can also be attributable to protection of endothelial cells from oxidative stress [201]. In addition, studies in mice indicate that resveratrol accelerates cutaneous wound healing by upregulation of vascular endothelial growth factor mediated by activation of at least two antioxidant enzymes (thioredoxin-1 and heme oxygenase-1) [123]. Because wound infections are the major cause of delayed wound healing [202], the antimicrobial properties of resveratrol could be another mechanism whereby wound healing is accelerated.…”

Section: Woundmentioning

confidence: 99%

See 2 more Smart Citations

Role of Resveratrol in Regulating Cutaneous Functions

Wen

Zhang

Yang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Protective role of the skin is against external insults and maintenance of electrolyte homeostasis of the body. Cutaneous dysfunction can account for the development of both cutaneous and systemic disorders. Thus, improvements in cutaneous functions can benefit a number of extracutaneous and cutaneous functions. Resveratrol, a natural ingredient, displays multiple benefits for various systems/organs, including the skin. The benefits of resveratrol for cutaneous functions include stimulation of keratinocyte differentiation and antimicrobial peptide expression, inhibition of keratinocyte proliferation and cutaneous inflammation, UV protection, anticancer, antiaging, and inhibition of melanogenesis. The mechanisms of action of resveratrol include activation of sirtuin 1 and nuclear factor erythroid 2-related factor 2, and inhibition of mitogen-activated protein kinase signaling. Evidence suggests that topical resveratrol could be a valuable alternative not only for daily skin care, but also for the prevention and treatment of various cutaneous disorders. This review summarizes the benefits of resveratrol for cutaneous functions.

show abstract

Section: Anti-inflammationmentioning

confidence: 77%

Section: Ratsmentioning

confidence: 99%

Section: Woundmentioning

confidence: 99%

See 1 more Smart Citation

Role of Resveratrol in Regulating Cutaneous Functions

Wen

Zhang

Yang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…As for experimental corneal neovascularization, the effect of RES is related to the way of administration, oral RES has anti-angiogenesis effect, while subconjunctival administration has no anti-angiogenesis effect (Doganay et al, 2013). On the contrary, RES promotes random skin flap survival and the ischemic wound healing through effective angiogenesis (Lakshmanan et al, 2019; Lin et al, 2019). Thus, the exact mechanisms of RES and its regulatory roles for angiogenesis, should be analyzed under specific circumstances.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Promotes Diabetic Wound Healing via SIRT1-FOXO1-c-Myc Signaling Pathway-Mediated Angiogenesis

et al. 2019

View full text Add to dashboard Cite

Background/Aims: Diabetic non-healing skin ulcers represent a serious challenge in clinical practice, in which the hyperglycemia-induced disturbance of angiogenesis, and endothelial dysfunction play a crucial role. Resveratrol (RES), a silent information regulator 1 (SIRT1) agonist, can improve endothelial function and has strong proangiogenic properties, and has thus become a research focus for the treatment of diabetic non-healing skin ulcers; however, the underlying mechanism by which RES regulates these processes remains unclear. Therefore, the present study was intended to determine if RES exerts its observed protective role in diabetic wound healing by alleviating hyperglycemia-induced endothelial dysfunction and the disturbance of angiogenesis. Methods:We investigated the effects of RES on cell migration, cell proliferation, apoptosis, tube formation, and the underlying molecular mechanisms in 33 mM high glucose-stimulated human umbilical vein endothelial cells (HUVECs) by semiquantitative RT-PCR, western blot analysis, terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL) staining, and immunofluorescence in vitro. We further explored the role of RES on endothelial dysfunction and wound healing disturbance in db/db mice by TUNEL staining, immunofluorescence, and photography in vivo. Results:We observed an obvious inhibition of hyperglycemia-triggered endothelial dysfunction and a disturbance of angiogenesis, followed by the promotion of diabetic wound healing via RES, along with restoration of the activity of the hyperglycemiaimpaired SIRT1 signaling pathway. Pretreatment with EX-527, a SIRT1 inhibitor, abolished the RES-mediated endothelial protection and pro-angiogenesis action, and then delayed diabetic wound healing. Furthermore, examination of the overexpression of forkhead box O1 (FOXO1), a transcription factor substrate of SIRT1, in HUVECs Frontiers in Pharmacology | www.frontiersin.org April 2019 | Volume 10 | Article 421 Huang et al.Resveratrol Promotes Diabetic Wound Healing and db/db mice revealed that RES activated SIRT1 to restore hyperglycemia-triggered endothelial dysfunction and disturbance of angiogenesis, followed by the promotion of diabetic wound healing in a c-Myc-dependent manner. Pretreatment with 10058-F4, a c-Myc inhibitor, repressed RES-mediated endothelial protection, angiogenesis, and diabetic wound healing. Conclusion:Our findings indicate that the positive role of RES in diabetic wound healing via its SIRT1-dependent endothelial protection and pro-angiogenic effects involves the inhibition of FOXO1 and the de-repression of c-Myc expression.

show abstract

“…The release of resveratrol was sustained, reaching 70% after 6 h. Application on full-thickness excision diabetic rat models resulted in improved collagen fibers as the addition of resveratrol delayed dermal matrix degradation by collagenases and prevented inflammation due to antioxidant properties. Lakshmanan et al [ 157 ] developed electrospun poly(ε-caprolactone)-based scaffolds containing resveratrol. In vivo experiments full-thickness ischemic mice wound models showed a considerably faster wound closure and re-epithelialization than the collagen-treated and negative control groups.…”

Section: Wound Dressings In Skin Cancermentioning

confidence: 99%

Regenerative Wound Dressings for Skin Cancer

Pavel

Chircov

Rădulescu

et al. 2020

Cancers

View full text Add to dashboard Cite

Skin cancer is considered the most prevalent cancer type globally, with a continuously increasing prevalence and mortality growth rate. Additionally, the high risk of recurrence makes skin cancer treatment among the most expensive of all cancers, with average costs estimated to double within 5 years. Although tumor excision is the most effective approach among the available strategies, surgical interventions could be disfiguring, requiring additional skin grafts for covering the defects. In this context, post-surgery management should involve the application of wound dressings for promoting skin regeneration and preventing tumor recurrence and microbial infections, which still represents a considerable clinical challenge. Therefore, this paper aims to provide an up-to-date overview regarding the current status of regenerative wound dressings for skin cancer therapy. Specifically, the recent discoveries in natural biocompounds as anti-cancer agents for skin cancer treatment and the most intensively studied biomaterials for bioactive wound dressing development will be described.

show abstract

Evaluation of dermal tissue regeneration using resveratrol loaded fibrous matrix in a preclinical mouse model of full-thickness ischemic wound

Cited by 30 publications

References 31 publications

Role of Resveratrol in Regulating Cutaneous Functions

Role of Resveratrol in Regulating Cutaneous Functions

Resveratrol Promotes Diabetic Wound Healing via SIRT1-FOXO1-c-Myc Signaling Pathway-Mediated Angiogenesis

Regenerative Wound Dressings for Skin Cancer

Contact Info

Product

Resources

About