Evaluation of desmoglein enzyme‐linked immunosorbent assay (ELISA) in Indian patients with pemphigus vulgaris

Sharma, Vinod; Prasad, Hari; Khandpur, Sujay; Kumar, Ashok

doi:10.1111/j.1365-4632.2006.02593.x

Cited by 52 publications

(82 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Only patients with a "classical" antibody profile (IIF + and anti-Dsg3 + in PV, IIF + and anti-Dsg1 + in PF, at least once during the course of the disease) were included in our study. Epidemiological data and antibody profiles of our patients were comparable to previous reports (2,22,(36)(37)(38)(39)(40), except for one female patient who presented with erosive, crusted, vegetating plaques exclusively on her scalp, but negative anti-Dsg1 values. Based on histological findings (suprabasal acantholysis), ELISA values (Dsg1 -/Dsg3 + ), and immunoprecipitation (single band at 130 kDa, consistent with Dsg3) we favoured the diagnosis of a cutaneous type PV (22,41), reminiscent of a previously published case described as Dsg1 -/ Dsg3 + pemphigus vegetans of the scalp (42), but lacking histological changes typical for this variant of PV such as papillomatosis, acanthosis and an abundance of eosinophils (43).…”

Section: Discussionsupporting

confidence: 87%

Autoantibody Levels and Clinical Disease Severity in Patients with Pemphigus: Comparison of Aggregated Anti-desmoglein ELISA Values and Indirect Immunofluorescence Titres

Weiss¹,

Ristl²,

Griss³

et al. 2015

Acta Derm Venerol

View full text Add to dashboard Cite

Detecting serum-autoantibodies by anti-Desmoglein-1 (anti-Dsg1) and anti-Dsg3 ELISAs as well as indirect immunofluorescence (IIF) are established complementary methods to diagnose pemphigus. Whether autoantibody levels also reflect clinical disease activity is still a matter of debate, as head-to-head comparisons of ELISA values and IIF titres with clinical activity over a longer treatment period are scarce. In our retrospective study, we compared aggregated repetitive intra-patient ELISA values and IIF titres with grades of clinical disease (1 = remission, 2 = moderate activity, 3 = exacerbation) in 47 patients suffering from pemphigus vulgaris (PV, n = 36) and pemphigus foliaceus (PF, n=11). We found that anti-Dsg1 ELISA values in PF and mucocutaneous PV as well as anti-Dsg3 ELISA values in PV best reflect disease activity. IIF titres, by contrast, did not show a significant association with disease severity. From these data we conclude that ELISA index values can be a valuable tool to monitor disease in patients with pemphigus, whereas IIF titres reflect clinical activity only insufficiently.

show abstract

Section: Discussionsupporting

confidence: 87%

Autoantibody Levels and Clinical Disease Severity in Patients with Pemphigus: Comparison of Aggregated Anti-desmoglein ELISA Values and Indirect Immunofluorescence Titres

Weiss¹,

Ristl²,

Griss³

et al. 2015

Acta Derm Venerol

View full text Add to dashboard Cite

show abstract

“…In our previous study also we observed that the ELISA test could not differentiate between the various clinical phenotypes of PV because both anti-Dsg 1 and 3 titers were raised in all the three variants. [12] Jamora et al reported a high anti-Dsg 1 titer in 28% of mucosal PV and its absence in 29% of the cutaneous variant. [13] Delmonte et al reported raised anti-Dsg 1 antibody in 62% of PV.…”

Section: Discussionmentioning

confidence: 98%

Comparison of enzyme-linked immunosorbent assay test with immunoblot assay in the diagnosis of pemphigus in Indian patients

Khandpur¹,

Sharma²,

Sharma³

et al. 2010

Indian J Dermatol Venereol Leprol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Anti-Dsg 3 titer correlated positively with mucosal severity of the disease, while anti-Dsg 1 titers correlated with skin surface area involvement. [23] Pemphigus being an autoimmune disease driven by autoantibody against epidermal antigen, it is expected from immunological point of view that Th2 predominance would be observed. It has been confirmed by Satyam et al in their study on serum samples from patients of either PV or PF.…”

Section: Genetic Susceptibility and Immunological Aspectmentioning

confidence: 99%

Pemphigus in India

Kanwar¹,

De²

2011

Indian J Dermatol Venereol Leprol

View full text Add to dashboard Cite

Pemphigus is a chronic epidermal immunobullous disease with potentially fatal outcome. The journey of literature on pemphigus in India has come a long way in last five decades. Pemphigus in Indian patients has unique genetic, clinical, and epidemiological differences from those in the Western countries. Corticosteroids remain the mainstay of treatment for pemphigus. Dexamethasone-cyclophosphamide pulse therapy has revolutionized the management of pemphigus in India and abroad for nearly 3 decades now. Corticosteroid-based treatment, along with adjuvants, has significantly brought down the high mortality rates that had been observed in precorticosteroid era. Present day research is largely based on elucidating the pathogenesis beyond the antidesmoglein antibodies, and newer diagnostic and treatment approaches. In this article, we review various aspects of literature on pemphigus in India, on Indians abroad, or literature from other countries that are considered relevant to the topic.

show abstract

Evaluation of desmoglein enzyme‐linked immunosorbent assay (ELISA) in Indian patients with pemphigus vulgaris

Abstract: ELISA (dsg1 and dsg3) is an efficient tool for confirming the diagnosis of PV. Specific antibody titers correlate with disease severity; however, desmoglein testing cannot differentiate between the various morphologic subtypes of PV.

Cited by 52 publications

References 13 publications

Autoantibody Levels and Clinical Disease Severity in Patients with Pemphigus: Comparison of Aggregated Anti-desmoglein ELISA Values and Indirect Immunofluorescence Titres

Autoantibody Levels and Clinical Disease Severity in Patients with Pemphigus: Comparison of Aggregated Anti-desmoglein ELISA Values and Indirect Immunofluorescence Titres

Comparison of enzyme-linked immunosorbent assay test with immunoblot assay in the diagnosis of pemphigus in Indian patients

Pemphigus in India

Contact Info

Product

Resources

About