Evaluation of diagnostic and quantitative PCR for the identification and severity assessment of eyespot and sharp eyespot in winter wheat

Nicholson, P.; Edwards, Simon; Bateman, G. L.; Morgan, L. W.; Todd, A. D.; Parry, D. W.; Marshall, Juliet M.; Nuttall, Michaela

doi:10.1046/j.1365-3059.2001.00592.x

Cited by 20 publications

(22 citation statements)

References 11 publications

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“…DNA in rye crop. As has been reported by Turner et al (2001), visual and PCR analyses on wheat stems generally supported each other, but there were often variances in relating disease incidence or the severity of symptoms to the amount of pathogen DNA by regression analyses; however, the relationships between symptoms and eyespot pathogen DNA were less clear on some cultivars. Ray at al.…”

Section: Discussionsupporting

confidence: 67%

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Ramanauskienė

Gaurilčikienė

Supronienė

et al. 2014

Zemdirbyste-Agriculture

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Eyespot of rye, caused by two fungal pathogens Oculimacula yallundae and O. acuformis, is widespread throughout many cool and wet cereal growing regions. The study was aimed to estimate eyespot incidence in winter rye crops in three agro-ecological zones of Lithuania, and to quantify eyespot disease causal agents O. yallundae and O. acuformis in the population using the real-time polymerase chain reaction (RT PCR) method. During the 2008-2012 periods, 69 winter rye fields were surveyed and eyespot-affected stems were identified in 92.7% of the crops surveyed. The incidence of eyespot in winter rye crops varied from 8.0% to 82.7% depending on the year and location. The average disease incidence in the reviewed crops was 26.0%. The highest incidence of eyespot averaging 34.0% was identified in the winter rye crops of the Middle Lowland zone. Both eyespot causal agents O. yallundae and O. acuformis were often found to co-exist on the winter rye stems. In the majority of the winter rye crops tested, O. acuformis was predominant in the Oculimacula spp. population. In most cases, O. yallundae was identified only at trace-level concentrations.

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Section: Discussionsupporting

confidence: 67%

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Ramanauskienė

Gaurilčikienė

Supronienė

et al. 2014

Zemdirbyste-Agriculture

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“…According to Turner et al (2001), visual and PCR analyses on stems varied in relation to disease incidence or severity of symptoms and to the amount of pathogen DNA. Ramanauskienė et al (2014) have previously reported that stem base and foot rot disease incidence did not correlate with fungal DNA amounts from diseased stems.…”

Section: Resultsmentioning

confidence: 99%

Microdochium nivale and M. majus as causative agents of seedling blight in spring cereals

Jonavičienė

Supronienė

Semaškienė

2016

Zemdirbyste-Agriculture

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Seedling blight, mostly caused by Fusarium spp. and Bipolaris sorokiniana, is a common disease in spring cereals. This research confirmed the presence of Microdochium fungi in a complex of seedling blight causative agents. Pathogens Microdochium nivale and M. majus in the seed and in stem base of seedlings of spring barley, spring wheat, spring triticale and spring oats were identified and quantified using a quantitative real-time polymerase chain reaction (qPCR) in 2013-2015. Both species were detected in the seed of all spring cereals tested. The amount of M. nivale and M. majus DNA was the highest in seeds of barley. Both Microdochium pathogens were present in the stem base of seedlings of all spring cereals tested; however, a high variation between cereal species and years was established. In most cases, the quantity of M. nivale DNA was the lowest in the seedlings of oats, while that of M. majus -in the seedlings of barley compared to the other cereal species tested. Higher contents of M. majus and M. nivale DNA were identified in the stem base of the seedlings emerged from untreated seeds compared with the seedlings emerged from the fludioxonil-treated seeds. However, the effect of tebuconazole on the reduction of M. nivale and M. majus DNA was inconsistent. Our findings suggest that M. nivale and M. majus occur in the seed of spring cereals and cause seedling blight, therefore research on these pathogens needs to be extended.

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“…The results of 3 years revealed no correlations between these indicators for different agro-ecological zones. The absence of significant relationships suggests that disease development is largely influenced by year and location, probably owing to climatic and agronomic differences [26].…”

Section: Discussionmentioning

confidence: 99%

Incidence of eyespot in winter wheat and quantification of the fungi Oculimacula acuformis and O. yallundae in Lithuania

Ramanauskienė

Gaurilčikienė

2016

J Plant Dis Prot

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Eyespot, caused by Oculimacula acuformis and O. yallundae, is one of the most damaging diseases in areas, including Lithuania, where mild and damp autumn and winter conditions favour the development and spread of the pathogens. The aim of this study was to estimate the incidence of eyespot in Lithuania and to use real-time polymerase chain reaction to determine the structure of Oculimacula fungi in winter wheat crops, which has not been previously established. From 2008 to 2012, 122 winter wheat fields in three different agro-ecological zones of Lithuania were reviewed for incidences of eyespot. Eyespot was identified in 95.9 % of the tested fields. Eyespot incidence in winter wheat varied depending on the experimental year and location, and the disease was spread over almost all of the tested fields. Depending on the year, the incidence of eyespot in different agro-ecological zones varied from 26.7 to 60.8 %. Both O. acuformis and O. yallundae were found to coexist on winter wheat stems. The amounts of Oculimacula spp. DNA varied between experimental years and sites. The highest amounts of both O. acuformis and O. yallundae were found in mid-lowland zones. For all years, higher averaged amounts of O. acuformis DNA were found in Middle Lithuania; however, for 2 years out of 3, averaged amounts of O. yallundae DNA were higher in eyespot-diseased winter wheat stems from western zones.

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Evaluation of diagnostic and quantitative PCR for the identification and severity assessment of eyespot and sharp eyespot in winter wheat

Cited by 20 publications

References 11 publications

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania

Microdochium nivale and M. majus as causative agents of seedling blight in spring cereals

Incidence of eyespot in winter wheat and quantification of the fungi Oculimacula acuformis and O. yallundae in Lithuania

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