Evaluation of Diagnostic Parameters to Detect Induction of Acne, Acute Dermal Irritation and Corrosion Potential of a Polyherbal Formulation

Abstract

The current protocol was designed to evaluate and detect the new parameters of acne induction (Histological lesion), Acute Dermal Irritation and corrosion potential of a polyherbal nano emulsion (obtained from research and development of Venus Medicine Research Centre, Baddi, H.P, India) according to Organisation for Economic Co-operation and Development (OECD) guidelines. Materials and Methods: Polyherbal drug, a new micro emulsion for topical use applied on the dorsal area of the trunk, has been developed as an anti-acne herbal nano-emulsion. Polyherbal drug's active ingredients include herbal extracts of Melaleuca alternifolia oil, Rosmarinus officinalis oil, Mentha arvensis oil along with Citrus limon are tested for their antibacterial, antimicrobial, anti-inflammatory and antiseptic effects. Serialise testing approach is recommended for developing scientifically sound data on the corrosivity/irritation of a new substance. Young healthy albino rabbits were used for the initial corrosivity/irritation study. If corrosive response was not found in the initial test, the irritant or negative response was confirmed using three additional animals each with one patch for an exposure period of four hours. Each animal served as its own control. After application of polyherbal emulsion the degree of irritation/corrosion and histological lesions were read and scored. Results: No severe erythema, oedema or any histological lesions were observed after polyherbal nanoemulsion application. The results revealed no irritation potential of polyherbal nanoemulsion. Conclusion: polyherbal nanoemulsion is safe formulation for topical use.