Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

Lizarazo, Yanedt Amparo Vera; Ferri, Elías Fernando Rodríguez; Gutiérrez-Martín, César B.

doi:10.1016/j.rvsc.2008.02.002

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…The results showed moderate biochemical variability among the P multocida isolates examined with 26 different profiles obtained using the API 20E system, as well as a significant percentage of isolates (21.7 per cent) that were not accurately identified with this method. Although this percentage is lower than the 36 per cent of misidentified isolates of P multocida obtained by Collins and others (1981), the present results confirm that the API 20E should not be considered a reliable system for the correct identification of P multocida isolates in clinical laboratories (Vera Lizarazo and others 2008). On the other hand, considering that all isolates were identified as P multocida using the PCR assay proposed by Townsend and others (1998), this genetic identification technique would represent a rapid, reliable and precise alternative to the phenotypic identification of P multocida isolates using commercial identification systems.…”

Section: Discussioncontrasting

confidence: 50%

Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain

Garcı́a

Fernández‐Garayzábal²,

Goyache³

et al. 2011

Veterinary Record

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Two hundred and five isolates of Pasteurella multocida from pigs were phenotypically and genetically characterised by determining their biovar, capsular type, virulence-associated genes and pulsed-field gel electrophoresis (PFGE) profiles. All isolates were identified as P multocida subspecies multocida and most were assigned to biovar 3 (58 per cent) and biovar 2 (39.5 per cent). Biovar 1 represented 2.4 per cent of the isolates. According to the capsular type, the great majority of the isolates (79.0 per cent) belonged to capsular type A, 18.5 per cent belonged to capsular type D and 2.4 per cent were of capsular type F. All isolates harboured ompH, psl, oma87, ptfA, nanB, nanH, tonB, hgbA, sodA and sodC genes, while none of them possessed the transferrin-binding protein gene tbpA. The prevalence of toxA, pfhaA and hgbB genes was variable (7.8, 40.5 and 60.5 per cent of the isolates, respectively). After PFGE typing, isolates of biovar 2 and 3 were grouped in two different clusters (A and B) at a level of 45 per cent similarity. In addition, isolates of biovar 2 and 3 exhibited statistically significant differences (P<0.05) in the virulence-associated hgbB and pfhA genes (biovar 3 was hgbB(+) pfhA(-), while biovar 2 was hgbB- pfhA(+)).

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Section: Discussioncontrasting

confidence: 50%

Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain

Garcı́a

Fernández‐Garayzábal²,

Goyache³

et al. 2011

Veterinary Record

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“…Eight isolates exhibited no growth on MacConkey agar, and they were correctly identifed as P. multocida by API ® 20NE with a numerical profle of 3000004 (% ID � 96%). Tis numerical profle is consistent with the results of previous studies [29,30]. Meanwhile, the other two isolates (468 and 492) grew pink bacterial colonies on MacConkey agar (Figure 1(c)).…”

Section: Bacterial Identifcationsupporting

confidence: 90%

“…Both of these characteristics are not accommodated by the API ® 20NE test, so they can cause misidentifcation if not tested separately. Cases of misidentifcation by API ® 20NE on P. multocida have also been reported in a previous study [30]. Te misidentifed isolate was identifed as Aeromonas salmonicida (% ID � 55.2%).…”

Section: Discussionsupporting

confidence: 69%

Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia

Desem

Handharyani

Setiyono

et al. 2023

Veterinary Medicine International

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Pasteurella multocida is a Gram-negative bacterium that causes hemorrhagic septicemia (HS) in buffaloes and cattle. The disease causes serious problems in Indonesian livestock and is classified as a serious transmissible animal disease. Previous research has determined the diversity of P. multocida using a serotyping method based on the antigenic properties of capsule polysaccharides. An alternative method for analysis utilizes sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD). This study aimed to characterize and determine P. multocida diversity in several regions of Indonesia based on phenotypic character, protein profile, and the band pattern of RAPD results. Bacterial identification was performed using traditional biochemical techniques and API® 20NE systems and then confirmed molecularly using polymerase chain reaction (PCR). The freeze-thawing technique was performed to obtain the bacterial protein extract, and DNA extraction was executed using DNAzol. The extracted protein and RAPD product were then electrophoresed on 12% polyacrylamide gel and 1.5% agarose gel, respectively. The results indicate that the molecular weight range of the protein bands is 12–209 kDa, and the band pattern of the RAPD results ranged from 307–3,100 bp. Based on phenotypical analysis, P. multocida from South Sulawesi Province exhibited a variety of growth characteristics in MacConkey agar media. Using the hierarchical clustering analysis of the band patterns of RAPD and the whole-cell protein profiles, four and five clusters were formed, respectively. These results indicate molecular diversity among P. multocida from several regions of Indonesia.

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“…Through API 50 CHL test, LS, LP, and L14 which were isolated from different products were identified as L. plantarum 1 (Figure 1). The API 50 CHL system can be useful for characterizing the bacteria below species level, but it has limitations that cannot identify all unknown bacteria [38]. For this reason, 16S rRNA sequencing was supplementally performed to clarify that the three LABs were L. plantarum (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Cell-Free Extracts from Three Newly Identified Lactobacillus plantarum Strains on the Inhibitory Effect of Adipogenic Differentiation and Insulin Resistance in 3T3-L1 Adipocytes

Lee²,

Kim

et al. 2021

BioMed Research International

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Obesity and associated metabolic disorders, including cardiovascular disease and diabetes, are rapidly becoming serious global health problems. It has been reported that Lactobacillus plantarum (L. plantarum) extracts have the beneficial activities of antiobesity and antidiabetes, although few studies have compared the beneficial effects among various L. plantarum extracts. In this study, three new L. plantarum (named LP, LS, and L14) strains were identified, and the antiobesogenic and diabetic effects of their extracts were investigated and compared using 3T3-L1 cells in vitro. Lipid accumulation in maturing 3T3-L1 cells was significantly decreased by the addition of LS and L14 extracts. The mRNA expression levels of Pparγ, C/ebpα, Fabp4, Fas, and Dgat1 were significantly decreased by the addition of LP, LS, and L14 extracts. Interestingly, the protein expression levels of PPARγ, C/EBPα, FABP4, and FAS were downregulated in mature 3T3-L1 cells with the addition of the L14 extract. Moreover, the LS and L14 extract treatments stimulated glucose uptake in maturing adipocytes. The L14 extract treatments exhibited a significant reduction in TNF-α protein expression, which is a key factor of insulin resistance in adipocytes. Of the three extracts, L14 extract markedly reduced adipogenic differentiation and insulin resistance in vitro, suggesting that the L14 extract may be used as a therapeutic agent for obesity-associated metabolic disorders.

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Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

Cited by 7 publications

References 11 publications

Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain

Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain

Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia

Comparison of Cell-Free Extracts from Three Newly Identified Lactobacillus plantarum Strains on the Inhibitory Effect of Adipogenic Differentiation and Insulin Resistance in 3T3-L1 Adipocytes

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