Evaluation of different cooling rates, equilibration periods and diluents for effects on deep-freezing, enzyme leakage and fertility of taurine bull spermatozoa

Dhami, A. J.; Sahni, K. L.

doi:10.1016/0093-691x(93)90297-i

Cited by 22 publications

(12 citation statements)

References 13 publications

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“…A greater value of STR indicates that the trajectory of sperm is more uniform and with low amplitude, was supported by earlier study [5]. The success of this technique is evident by the fact that the overall field conception rate was observed to be 55.2 % which is almost equal to fertility rate (57.9 % on 90-d NRR) observed in cattle [2,3], however, it was slightly lower than that observed by Singleton [9].…”

Section: Discussionsupporting

confidence: 46%

“…In the present study, no significant effect of post-collection equilibration (2-4 h) during transportation on total motility and progressive motility of frozen-thawed semen was obtained. Earlier reports also found that 2-4 h equilibration improved the total and progressive motility and fertility of frozen-thawed semen in bubaline [2,3]. Based on the results of slow cooling and equilibration time at 5°C and its relationship with fertility, several authors have recommended an equilibration of 4-18 h at 5°C before freezing [4,6].…”

Section: Discussionmentioning

confidence: 99%

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Cryopreservation and Quality Assessment of Buffalo Bull Semen Collected from Farmer’s Doorstep

Singh

Kumar

et al. 2013

Agric Res

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The present study was undertaken with the objective to evaluate the effect of transportation distance on freezability of buffalo semen collected at farmer's doorstep. In the study elite Murrah buffalo bulls of 3-6 years of age available with the farmers in the breeding home tract were used. Semen samples were divided into two groups based on distance of transportation viz. group I (80-100 km) and group II (130-150 km). Semen was collected at the owner's premises and kept in warm water at 30 ± 2°C and extended 10-15 times based on its consistency and color. The extended semen was transported to laboratory in indigenously designed container. In the laboratory, after subjective assessment, the semen was cryopreserved successfully. The frozen-thawed semen was assessed for quality parameters using computer assisted semen analyzer and artificial insemination (AI) in field conditions. Post-thaw sperm total motility (51.06 ± 2.59 vs. 45.36 ± 2.43 %) and progressive motility (31.8 ± 1.75 vs. 26.5 ± 1.55 %) did not vary significantly between groups, whereas rapid motility and sperm kinetic parameters like average path velocity, straight linear velocity, curvilinear velocity, beat cross frequency, and straightness were significantly (P \ 0.05) higher in group I than the group II. Parameters, such as amplitude of lateral head displacement, linearity, elongation, and head size of spermatozoa did not vary significantly between the two transportation distances. The conception rates, of the frozen semen used for AI at field level, were found to be 53.8 and 60.2 % in group I and group II, respectively. In conclusion, the proposed design of container for cooling and transportation of diluted semen, up to 150 km distance proved to be highly efficient for cryopreservation of semen of buffalo bulls from field conditions.

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Section: Discussionsupporting

confidence: 46%

Section: Discussionmentioning

confidence: 99%

Cryopreservation and Quality Assessment of Buffalo Bull Semen Collected from Farmer’s Doorstep

Singh

Kumar

et al. 2013

Agric Res

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“…Narasimha Rao et al (1986) reported ice crystals must transition from solid state to liquid state without going into different forms as they pass through the critical temperature limit. In the field, it is advised that bull spermatozoa are thawed in a water bath at 35°C for at least 30 sec (Nur et al, 2003;Dhami and Sahni, 1993;Hayashi and Isobe, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Thawing Time and High Temperature on Frozen Thawed Bull Semen Traits

Yılmaz¹,

Ak²,

Baran³

2020

J. Anim. Vet. Adv.

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In this study was to investigate the effect of high temperature thawing and post-thaw cold shock application on sperm motility as well as acrosomal and total abnormalities of frozen bull semen. Four Holstein bulls were used to frozen semen in 0.25 mL straws. Semen of each bulls were thawed in 45 sec at 37°C (Group A = Control group) in 15 sec at 50°C (Group B) and 5 sec at 70°C (Group C) and post-thawing cold shock (300 sec at 5°C) were applied to all groups. After spermatozoa motility and morphological examinations are performed sperm samples were incubated at 35°C for 120 min and spermatological traits were repeated. Semen samples from the control and treatment groups were placed in an incubator taking into consideration the medium conditions (Modified buffered Hepes medium) and the time needed for spermatozoa to reach the fertilization site. Motility and morphological defects rates were determined with phase contrast microscopy and motility rate and speed with sperm fertility analyser (SFA-500). The Hancock's solution was used for morphologic examination of spermatozoa (acrosome, other and total). In computer aided sperm fertility analyser, motility and movement were performed in according to the technique. In conclusion, it has been found that the conventional thawing method and rapid thawing technique are successful methods where the thawing method applied to Group B damaged spermatozoon viability. The rate of thawing in Group B did not protect the spermatozoa from dissolving or from the harmful effects of cold shock.

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“…According to Dhami and Sahni (), the time of spermatozoa exposed to the cryoprotectant prior to freezing affects its post‐thaw characteristics, as evidenced by better bovine sperm motility after 2 hr of equilibration compared to a protocol with no equilibration period. However, this hypothesis may not hold true for every species.…”

Section: Introductionmentioning

confidence: 99%

Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis

et al. 2016

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This work aimed to evaluate the effect of stabilisation times, glycerol concentration, and the catalase and superoxide dismutase supplementation of diluent on parameters of frozen-thawed spermatozoa from epididymis of Nelore bulls: Experiment 1: spermatozoa diluted in Tris-egg yolk with glycerol (3%, 5% or 7%) and stabilisation times (0, 2 or 4 hr at 5°C); Experiment 2: Tris-egg yolk only, Tris-egg yolk with catalase (CAT, 50 or 100 U ml ) or superoxide dismutase (SOD, 50 or 100 U ml ). Frozen-thawed spermatozoa were evaluated for kinetic parameters, plasma membrane and acrosome integrity, mitochondrial activity and IVF capacity. ALH and BCF were affected (p < .05) by glycerol at 3% after 4-hr equilibration time and 7% after 2-hr equilibration time. Glycerol 3% had lower (p < .05) iPM and iAc after 4 hr. Glycerol 5% had greater (p < .05) hPMM after 4 hr and iAc after 2 hr than at 0 hr. SOD 100 U ml had lower (p < .05) linearity and wobble compared to control group. No was observed differences to fertilisation rate (p < .05) among groups. In conclusion, glycerol 5% in Tris-egg yolk extender for 4 hr is suitable for the preservation of sperm kinetics and membrane integrity. CAT (50 and 100 U ml ) or SOD (50-100 U ml ) had no beneficial effects on sperm kinetics, plasma and acrosomal membrane integrity, mitochondrial activity or the capacity for IVF of frozen-thawed spermatozoa from epididymis of Nelore bulls.

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Evaluation of different cooling rates, equilibration periods and diluents for effects on deep-freezing, enzyme leakage and fertility of taurine bull spermatozoa

Cited by 22 publications

References 13 publications

Cryopreservation and Quality Assessment of Buffalo Bull Semen Collected from Farmer’s Doorstep

Cryopreservation and Quality Assessment of Buffalo Bull Semen Collected from Farmer’s Doorstep

Effect of Different Thawing Time and High Temperature on Frozen Thawed Bull Semen Traits

Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis

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