Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Tsai, Ming‐Hsun; Lee, Wen‐Chien; Kuan, Wei‐Chih; Sirisansaneeyakul, Sarote; Savarajara, Ancharida

doi:10.1002/ese3.243

Cited by 42 publications

(43 citation statements)

References 26 publications

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“…In addition, the time required for the SSF process is shorter, leading to a higher productivity [28]. SSF has been widely investigated for ethanol production from various biomass, including Napier grass [29], cornstalks [30], and cogongrass [31]. However, no reports have been found for its use on microalgal biomass for hydrogen production.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

et al. 2019

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Simultaneous saccharification and fermentation (SSF) and pre-hydrolysis with SSF (PSSF) were used to produce hydrogen from the biomass of Chlorella sp. SSF was conducted using an enzyme mixture consisting of 80 filter paper unit (FPU) g-biomass−1 of cellulase, 92 U g-biomass−1 of amylase, and 120 U g-biomass−1 of glucoamylase at 35 °C for 108 h. This yielded 170 mL-H2 g-volatile-solids−1 (VS), with a productivity of 1.6 mL-H2 g-VS−1 h−1. Pre-hydrolyzing the biomass at 50 °C for 12 h resulted in the production of 1.8 g/L of reducing sugars, leading to a hydrogen yield (HY) of 172 mL-H2 g-VS−1. Using PSSF, the fermentation time was shortened by 36 h in which a productivity of 2.4 mL-H2 g-VS−1 h−1 was attained. To the best of our knowledge, the present study is the first report on the use of SSF and PSSF for hydrogen production from microalgal biomass, and the HY obtained in the study is by far the highest yield reported. Our results indicate that PSSF is a promising process for hydrogen production from microalgal biomass.

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Section: Introductionmentioning

confidence: 99%

Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

et al. 2019

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“…However, the utilisation of biomass resources for electricity, biofuel, or biogas generation has not been extensively utilised or studied in most African countries [9][10][11]. From the different lignocellulosic materials currently available in Nigeria, Napier grass has been reported to be a particularly attractive feedstock for production of biofuels and bio-based products mainly due to its high cellulose content (34.2-40%), high yields per unit area, tolerance to drought, and a good water use efficiency (ratio of water used by the crop to water lost by evapotranspiration) [12][13][14][15][16][17].Napier grass is a perennial C4 plant endemic to Sub-Saharan Africa with a high heating value biomass (16.58 MJ/kg) [18]. This crop is mainly used to feed cattle, but it can also be used for grazing, silage, or hay production or fish food [15].…”

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confidence: 99%

Bioenergy Yields from Sequential Bioethanol and Biomethane Production: An Optimized Process Flow

et al. 2019

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This study investigates the potential of different stages of the bioethanol production process (pretreatment, hydrolysis, and distillation) for bioethanol and biomethane production, and studies the critical steps for the liquid and the solid fractions to be separated and discarded to improve the efficiency of the production chain. For this, Napier grass (a fast-growing grass) from Effurun town of Delta State in Nigeria was used and the novel pretreatment method, nitrogen explosive decompression (NED), was applied at different temperatures. The results show that the lowest glucose (13.7 g/L) and ethanol titers (8.4 g/L) were gained at 150 • C. The highest glucose recovery (31.3 g/L) was obtained at 200 • C and the maximum ethanol production (10.3 g/L) at 170 • C. Methane yields are higher in samples pretreated at lower temperatures. The maximum methane yields were reported in samples from the solid fraction of post-pretreatment (pretreated at 150 • C, 1.13 mol CH4/100 g) and solid fraction of the post-hydrolysis stage (pretreated at 150 • C, 1.00 mol CH4/100 g). The lowest biomethane production was noted in samples from the liquid fraction of post-pretreatment broth (between 0.14 mol CH4/100 g and 0.24 mol CH4/100 g). From the process point of view, samples from liquid fraction of post-pretreatment broth should be separated and discarded from the bioethanol production process, since they do not add value to the production chain. The results suggest that bioethanol and biomethane concentrations are influenced by the pretreatment temperature. Napier grass has potential for bioethanol and further biomethane production and it can be used as an alternative source of energy for the transportation sector in Nigeria and other countries rich in grasses and provide energy security to their population.alternative sources of energy that will decrease the share of fossil fuels utilised in the transportation sector and productive uses, reduce environmental concerns caused by the extensive utilization of these resources (e.g., climate change and global warming), increase energy security, and improve the access of the population to electricity. Renewable energy sources from lignocellulosic biomass have been reported as promising solutions to these problems [4]. Nowadays, biomass itself is responsible for 10% primary energy consumption worldwide [5] and it can be used as a promising feedstock for biofuel production. The sustainability of wastes and by-products as a biofuel for the transportation sector in a circular economy has been studied in the literature, in order to make the biogas-biomethane chain more sustainable [6][7][8]. These studies show the positive impact of biofuels in the transportation section in Europe. However, further research needs to be done in order to apply circular economy models to emerging economies.Sub-Saharan Africa has great potential to develop renewable energy sources, such as wind, biomass, solar, and hydro. Just in Nigeria, the biomass potential is about 144 million tonnes per year and the potenti...

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“…While annual dry matter yields of 13~21 t/ha have been obtained from some traditional agricultural wastes, yields of Arundo donax L. have reached 30~40 t/ha. These values are similar to that of energy crops such as Pennisetum grass (0~50.2 t/ha) and miscanthus (27~38 t/ha) [9][10][11]. Given its high yield, low cost, and environmentally benign production, Arundo donax L. is considered as a potential candidate for biofuel production, and hence, it was chosen as a substrate in this study to investigate its potential for bioconversion.…”

Section: Introductionmentioning

confidence: 68%

One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L.

Tang

Cao

et al. 2020

Energies

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Energy crops are not easily converted by microorganisms because of their recalcitrance. This necessitates a pretreatment to improve their biodigestibility. The effects of different pretreatments, as well as their combination on the enzymatic digestibility of Arundo donax L. were systematically investigated to evaluate its potential for bioconversion. Dilute alkaline pretreatment (ALP) using 1.2% NaOH at 120 °C for 30 min resulted in the highest reducing sugar yield in the enzymatic hydrolysis process because of its strong delignification and morphological modification, while ferric chloride pretreatment (FP) was effective in removing hemicellulose and recovering soluble sugars in the pretreatment stage. Furthermore, an efficient two-step ferric chloride-alkaline pretreatment (FALP) was successfully developed. In the first FP step, easily degradable cellulosic components, especially hemicellulose, were dissolved and then effectively recovered as soluble sugars. Subsequently, the FP sample was further treated in the second ALP step to remove lignin to enhance the enzymatic hydrolysis of the hardly degradable cellulose. As a result, the integrated two-step process obtained the highest total sugar yield of 420.4 mg/g raw stalk in the whole pretreatment and enzymatic hydrolysis process; hence, the process is a valuable candidate for biofuel production.

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Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Cited by 42 publications

References 26 publications

Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

Bioenergy Yields from Sequential Bioethanol and Biomethane Production: An Optimized Process Flow

One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L.

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