Evaluation of disease severity caused by <i>Bean golden mosaic virus</i> in different bean cultivars

Freitas-Vanzo, Aline Taiane de; Silva, Camila de Cássia da; Novaes, Tanara Garcia de; Walz, Daniel Mazzieri; Guimarães, Francismar Corrêa Marcelino; Kuwahara, Marcia Kamogae; Molina, Rúbia de Oliveira; Leite, Rui Pereira

doi:10.1080/07060661.2020.1771618

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Campos et al (2019) reported that qRT-PCR based on SYBR Green methodology produced high Ct values in low titres of olive viruses. Similarly, de Freitas-Vanzo et al (2021) used the qRT-PCR to quantify BGMV titre in host plants and found variations in symptom expression and BGMV titre between cultivars and lineages at 10 and 25 pi and the bean cultivar "Tangará" as reported in YLV-14 in this research, had a higher Ct value but showed less symptom severity than the other cultivars. Similar studies have also been carried out to investigate viral load that indicated the resistance and susceptibility of many hosts against various plant viruses, including BYDV, WYMV, TSWV, CBSD, and various grapevine viruses, which results were in agreement with the outcomes reported in this study (Gil salas et al, 2009;Jarošová and Kundu, 2010;Liu et al, 2013;Soler et al, 2015;Fajardo et al, 2017;Shirima et al, 2017).…”

Section: Discussionmentioning

confidence: 59%

A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris

et al. 2023

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Accurate and early diagnosis of bean common mosaic virus (BCMV) in Phaseolus vulgaris tissues is critical since the pathogen can spread easily and have long-term detrimental effects on bean production. The use of resistant varieties is a key factor in the management activities of BCMV. The study reported here describes the development and application of a novel SYBR Green-based quantitative real-time PCR (qRT-PCR) assay targeting the coat protein gene to determine the host sensitivity to the specific NL-4 strain of BCMV. The technique showed high specificity, validated by melting curve analysis, without cross-reaction. Further, the symptoms development of twenty advanced common bean genotypes after mechanical BCMV-NL-4 infection was evaluated and compared. The results showed that common bean genotypes exhibit varying levels of host susceptibility to this BCMV strain. The YLV-14 and BRS-22 genotypes were determined as the most resistant and susceptible genotypes, respectively, in terms of aggressiveness of symptoms. The accumulation of BCMV was analyzed in the resistant and susceptible genotypes 3, 6, and 9 days following the inoculation by the newly developed qRT-PCR. The mean cycle threshold (Ct) values showed that the viral titer was significantly lower in YLV-14, which was evident in both root and leaf 3 days after the inoculation. The qRT-PCR thus facilitated an accurate, specific, and feasible assessment of BCMV accumulation in bean tissues even in low virus titers, allowing novel clues in selecting resistant genotypes in the early stages of infection, which is critical for disease management. To the best of our knowledge, this is the first study of a successfully performed qRT-PCR to estimate BCMV quantification.

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Section: Discussionmentioning

confidence: 59%

A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris

et al. 2023

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“…In addition to the visual symptoms, it is important to note that the golden mosaic virus can seriously affect crop yield, impacting the quality and quantity of produced grains [87]. Therefore, early monitoring and the adoption of appropriate control measures are crucial to mitigate the damage caused by this disease.…”

Section: Biocontrol Agentsmentioning

confidence: 99%

“…Over time, these spots spread throughout the leaf blade or even throughout the entire plant (Figure 4), remaining limited by the veins, creating a characteristic mosaic appearance. In addition to the visual symptoms, it is important to note that the golden mosaic virus can seriously affect crop yield, impacting the quality and quantity of produced grains [87]. Therefore, early monitoring and the adoption of appropriate control measures are crucial to mitigate the damage caused by this disease.…”

Section: Biocontrol Agentsmentioning

confidence: 99%

Microbial Insights into Biofortified Common Bean Cultivation

Cardoso,

da Silva,

de Pádua

2024

Sci

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Microorganisms play a fundamental role in sustainable agriculture, and their importance in common bean (Phaseolus vulgaris) cultivation cannot be underestimated. This review article aims to comprehensively explore the diverse roles of microorganisms in sustainable biofortified common bean cultivation. Biofortification refers to the process of increasing the nutrient content in crops, which helps combat deficiencies in iron, zinc, and vitamins in the human body. Biofortified beans have better agronomic characteristics and offer higher micronutrient content compared to conventional crops. We examine the contribution of various microbial communities in nitrogen fixation, soil structure improvement, nutrient recycling, and disease suppression. Understanding the interaction between beneficial microorganisms and biofortified common bean plants enables us to develop ecologically sound and sustainable approaches to optimize crop productivity and improve nutrition and livelihoods for millions of people worldwide while reducing the environmental impact of agricultural practices.

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Interactions between Common Bean Viruses and Their Whitefly Vector

Ferreira,

Ghanim,

et al. 2024

Viruses

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Common bean (Phaseolus vulgaris L.) is a widely cultivated crop, representing an important protein source in the human diet in developing countries. The production of this crop faces serious challenges, such as virus diseases transmitted by the whitefly Bemisia tabaci. Although there is a lot of information about some of these viruses, most of what we know has been developed using model systems, such as tomato plants and tomato yellow leaf curl virus (TYLCV). There is still very little information on the most relevant common bean viruses, such as bean golden mosaic virus (BGMV), bean golden yellow mosaic virus (BGYMV), bean dwarf mosaic virus (BDMV), cowpea mild mottle virus (CPMMV), and bean yellow disorder virus (BnYDV). In this review, we discuss the available data in the most up-to-date literature and suggest future research avenues to contribute to the development of management tools for preventing or reducing the damage caused by viruses in this important crop.

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Evaluation of disease severity caused by Bean golden mosaic virus in different bean cultivars

Cited by 3 publications

References 24 publications

A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris

A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris

Microbial Insights into Biofortified Common Bean Cultivation

Interactions between Common Bean Viruses and Their Whitefly Vector

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