Evaluation of Diuretic activity of Fractional Extracts of Ajuga remota Benth (Lamiaceae) in Albino mice

Abstract

The present study was evaluated the in vivo diuretic activity of fractional extracts of A. remota in albino mice. The dried aqueous crude extracts were subjected to soxhlet extraction by n-butanol, methanol and water solvents. The mice were randomly divided into eleven groups with 8 mice in each. All fractions were administered orally at doses of 250, 500 and 1000 mg/kg to adult male mice, and the positive and negative controls were treated with furosemide (10 mg/kg, p.o) and the vehicle distilled water (2 ml/100 gm of body weight) respectively. The diuretic effect of the extracts was evaluated by measuring urine volume, urinary electrolytes and urinary pH. The result indicates that aqueous and methanolic fractions at 1000 mg/kg dose produced significant (p<0.001) increase in urine output and electrolyte excretion (p<0.001) when compared to control. Additionally, potassium sparing activity (27%, p<0.05) and high natriuretic index (2.7-3.03) were produced by the n-butanol fraction relatively even if it showed minimal effect on urine output. Therefore, from the present study it may be concluded that the compounds present in methanolic and aqueous fraction are responsible for diuretic activity. This finding together with previous results on the aqueous crude extracts provides a quantitative basis for developing a new diuretic medicine from A. remota plant.

Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania

“…A. integrifolia Buch., A. remota Benth.) leaves and aerial parts (rich in phenolic acids) were found as potent in vivo diuretics in rodent studies (Hailu and Engidawork, 2014;Chilo and Raju, 2021). Diuretic action measured in a rat study for A. remota fractions (in doses of 250, 500 and 1,000 mg/kg) revealed values between 1.05 and 2.53, while diuretic activity was shown as varying between 0.41 and 0.99 in comparison with positive control, furosemide respectively (Chilo and Raju, 2021).…”

“…leaves and aerial parts (rich in phenolic acids) were found as potent in vivo diuretics in rodent studies (Hailu and Engidawork, 2014;Chilo and Raju, 2021). Diuretic action measured in a rat study for A. remota fractions (in doses of 250, 500 and 1,000 mg/kg) revealed values between 1.05 and 2.53, while diuretic activity was shown as varying between 0.41 and 0.99 in comparison with positive control, furosemide respectively (Chilo and Raju, 2021). According to previous published works (Gujral et al, 1955;Hailu and Engidawork, 2014), a good diuretic activity is described by values above 1.5, but as long as there are no other reports regarding diuretic effects of other thyme species, we prefer to be cautious and define T. comosus as a mild diuretic agent, this statement being a good start point for further confirmations regarding this effect by human studies.…”