Evaluation of DNA damage in exfoliated tear duct epithelial cells from individuals exposed to air pollution assessed by single cell gel electrophoresis assay

Rojas, Emilio; Valverde, Mahara; López, Marcos Valdivia; Naufal, I; Sánchez, I. H.; Bizarro, Patricia; López, Irma Araceli Aburto; Fortoul, Teresa I.; Ostrosky‐Wegman, Patricia

doi:10.1016/s1383-5718(00)00035-8

Cited by 54 publications

(36 citation statements)

References 10 publications

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“…To the best of our knowledge we did not find any study showing association of CYP1A1 gene polymorphism with MN in exfoliated buccal epithelial cells in PAHs exposed population. As in our results CYP1A1 variant genotype has been found to be associated with higher levels of DNA damage in different occupational workers such as coke-oven workers (Brescia et al, 1999;Rojas et al, 2000), pot-room workers (Carstensen et al, 1999), traffic policemen (Carere et al, 2002). In our previous study we observed significant association between GSTM1 and GSTT1 null genotypes and BMN frequencies among coal-tar population (Kumar et al, 2011).…”

Section: Discussionsupporting

confidence: 83%

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Giri

Yadav²,

Kumar

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Aim: It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coaltar workers exposed to PAHs at their work place. Methods: Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects. Results: Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects. Conclusion: In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt)+homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.

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Section: Discussionsupporting

confidence: 83%

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Giri

Yadav²,

Kumar

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…The name refers to the fact that the head has no DNA damage while the tail is the fragmented DNA. Rojas et al (2000) used the comet assay in exfoliated tear duct epithelial cells of young adults, residents of the Northern and the Southern areas of MCMA; individuals in the last area showed the highest frequencies of DNA damage by the environmental pollution exposure. Gutiérrez-Castillo et al (2006) made two fractions with the particle matter (PM), one a water soluble and the other an organic soluble.…”

Section: Toxic Effects and Human Health Riskmentioning

confidence: 99%

Organic Compound in Airborne Particles and their Genottoxic Effects in Mexico City

Villalobos-Pietrini¹,

Amador-Muñóz²,

Valle-Hernández³

et al. 2011

Air Quality Monitoring, Assessment and Management

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“…Valverde et al [23] reported higher levels of damage in leukocytes and nasal epithelial cells from residents of south Mexico City compared with the north (but no difference for buccal epithelial cells). In tear duct cells, too, damage was higher (by about a third) when collected in the south of the city (with high levels of ozone) than in the north (with elevated levels of hydrocarbons in the air) [16]. DNA SBs in nasal mucosa cells in Florence, with a high level of ozone, were high in comparison with samples collected in Sardinia [24].…”

Section: Comet Assaymentioning

confidence: 85%

“…[15]), and a few on other cell types (e.g. [16]). The most common approach is to compare similar population groups living in polluted and clean areas.…”

Section: Discussionmentioning

confidence: 99%

Atmospheric pollution in Kosovo is associated with increased DNA damage in the human population

Alija¹,

Asllani²,

Bajraktari³

et al. 2016

Biomonitoring

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Abstract:In order to assess DNA damage associated with exposure to environmental pollution in two polluted sites and one control site in Kosovo, whole blood samples were collected from volunteers in two polluted areas (Kastriot/ Obiliq - lignite-based power plants and lignite mines - and Drenas/Gllogovc - Ferronikeli smelting plant) as well as from Peja, representing an unpolluted area. White blood cells were isolated, and DNA damage was analyzed by the alkaline comet assay. Significantly higher levels of DNA damage (strand breaks) were found in white blood cells from subjects living in the polluted areas compared with residents of the unpolluted city, indicating a potential threat to human health.

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Evaluation of DNA damage in exfoliated tear duct epithelial cells from individuals exposed to air pollution assessed by single cell gel electrophoresis assay

Cited by 54 publications

References 10 publications

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Organic Compound in Airborne Particles and their Genottoxic Effects in Mexico City

Atmospheric pollution in Kosovo is associated with increased DNA damage in the human population

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