Evaluation of DNA damage in workers occupationally exposed to pesticides using single-cell gel electrophoresis (SCGE) assay

Dose and Time-dependent Alterations in Various Cholinergic and Non-cholinergic Markers after Organophosphate Poisoning: Possible Role in Diagnosis

“…The complex can easily dissociate in the presence of OP to release high levels of BG into the plasma 15,16 . Measurement of antioxidants enzyme, certain cytogenetic biomarkers such as chromosomal aberration, sister chromatid exchange, DNA fragmentation and SCGE have also been used for the detection of exposure to commonly used OP pesticides 11,[29][30][31] . SCGE is a micro gel electrophoresis technique which detects DNA damage in individual cells 30 .…”

“…Measurement of antioxidants enzyme, certain cytogenetic biomarkers such as chromosomal aberration, sister chromatid exchange, DNA fragmentation and SCGE have also been used for the detection of exposure to commonly used OP pesticides 11,[29][30][31] . SCGE is a micro gel electrophoresis technique which detects DNA damage in individual cells 30 . However, most of these studies have been limited to OP pesticide exposures, and not applied to more toxic OP compounds like DFP and nerve agents.…”

Genotoxicity Testing in Pesticide Safety Evaluation

“…In last two decades comet assay has been adapted by many workers to evaluate the genotoxicity potentialities of pesticides in human populations (Garaj-Vrhovac & Zeljezic, . During a study on workers involved in the production of a variety of pesticides, an increase in DNA damage in peripheral blood lymphocytes was found (Garaj-Vrhovac & Zeljezic, 2000). In another experiment on Croatian workers occupationally exposed to a complex mixture of pesticides showed an increase in the values of the comet assay parameters (Garaj-Vrhovac & Zeljezic, 2001) indicating that the pesticides to be the potent genotoxic substances.…”

Pesticides: Genotoxic Risk of Occupational Exposure

“…Each damaged cell has the appearance of a comet with head and tail bright; the undamaged cells appear intact or with complete nuclei and no tail (Möller, 2006). The application of the comet assay to evaluate the DNA damage, biomontoring in populations occupationally exposed to pesticides has demonstrated positive results (Lebailly et al, 1998a,b;Garaj-Vrhovac & Zeljezic, 2000Zeljezic & Garaj-Vrhovac, 2001;Ündeğer & Başaran, 2002;Grover et al, 2003;Ascarrunz et al, 2006;Castillo-Cadena et al, 2006;Remor et al, 2009;Rohr et al, 2010). Besides, correlation between the CoA and exposure time was not observed or determined in any of these cases.…”