Evaluation of donor arm disinfection techniques

McDonald, Carl; Lowe, Peter; Roy, Anjana; Robbins, S.; Hartley, Stephen W.; Harrison, John P.; Slopecki, A.; Verlander, Neville Q.; Barbara, J. A. J.

doi:10.1046/j.1423-0410.2001.00029.x

Cited by 88 publications

(94 citation statements)

References 9 publications

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“…Sample collection was performed 3 and 5 days after CVC insertion, before dressing changes, using sterile and disposable swabs packaged in individual swab containers (Absorve ® , Carl, São Paulo, Brazil). The swab was previously moistened in a sterile phosphate-buffered saline (PBS buffer) containing 3% Tween-80 18 , and rubbed for 30 seconds with uniform up and down movements in a 5 cm 2 area delineated by a sterile filter paper template (Nalgon ® , Nalgon Equipamentos Científicos Ltda, São Paulo, Brazil) 19 . The swab was then placed in sterile tubes containing 1 ml of 0.9% saline solution and 8 glass beads, and transported in a thermal box to the Laboratory of Basic Research of the School of Health Sciences at UNIVÁS.…”

Section: Evaluation Of the Microbiological Culturementioning

confidence: 99%

Fixation of the short-term central venous catheter. A comparison of two techniques

et al. 2017

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Fixation of the short-term central venous catheter. A comparison of two techniques 1Abstract Purpose: To compare the fixation of the central venous catheter (CVC) using two suture techniques. Methods: A clinical, analytical, interventional, longitudinal, prospective, controlled, singleblind and randomized study in adult, intensive care unit (ICU) patients. After admission and indication of CVC use, the patients were allocated to the Wing group (n = 35, catheter fixation with clamping wings and retainers) or Shoelace group (n = 35, catheter fixation using shoelace cross-tied sutures around the device). Displacement, kinking, fixation failure, hyperemia at the insertion site, purulent secretion, loss of the device, psychomotor agitation, mental confusion, and bacterial growth at the insertion site were evaluated. Results: Compared with the Wing group, the Shoelace group had a lower occurrence of catheter displacement (n=0 versus n =4; p = 0.04), kinking (n=0 versus n=8; p=0.001), and fixation failure (n=2 versus n=8; p=0.018). No significant difference was found in bacterial growth (n=20 versus n=14; p=0.267) between groups. Conclusion:The Shoelace fixation technique presented fewer adverse events than the Wing fixation technique.

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Section: Evaluation Of the Microbiological Culturementioning

confidence: 99%

Fixation of the short-term central venous catheter. A comparison of two techniques

et al. 2017

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“…52,53 Recent studies exploring blood culture contamination rates of blood products have demonstrated that both the quality of the antiseptic used and/or the mode of application of the antiseptic can influence the efficacy of skin disinfection. Thus, one possibility of reducing the prevalence of blood product bacterial contamination might be to improve donor skin disinfection.…”

Section: Improving Donor Skin Disinfectionmentioning

confidence: 99%

Bacterial Contamination of Blood Components: Risks, Strategies, and Regulation

Hillyer

Josephson²,

Blajchman³

et al. 2003

Hematology

221

210

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Bacterial contamination of transfusion products, especially platelets, is a longstanding problem that has been partially controlled through modern phlebotomy practices, refrigeration of red cells, freezing of plasma and improved materials for transfusion product collection and storage. Bacterial contamination of platelet products has been acknowledged as the most frequent infectious risk from transfusion occurring in approximately 1 of 2,000-3,000 whole-blood derived, random donor platelets, and apheresis-derived, single donor platelets. In the US, bacterial contamination is considered the second most common cause of death overall from transfusion (after clerical errors) with mortality rates ranging from 1:20,000 to 1:85,000 donor exposures. Estimates of severe morbidity and mortality range from 100 to 150 transfused individuals each year.Concern This Joint ASH and AABB Educational Session reviews the risks, testing strategies, and regulatory approaches regarding bacterial contamination of blood components to aid in preparing practitioners of hematology and transfusion medicine in understanding the background and clinical relevance of this clinically important issue and in considering the approaches currently available for its mitigation, as well as their implementation.In this chapter, Drs. Hillyer and Josephson review the background and significance of bacterial contamination, as well as address the definitions, conceptions and limitations of the terms risk, safe and safety. They then describe current transfusion risks including non-infectious serious hazards of transfusion, and current and emerging viral risks. In the body of the text, Dr. Blajchman reviews the prevalence of bacterial contamination in cellular blood components in detail with current references to a variety of important studies. He then describes the signs and symptoms of transfusion-associated sepsis and the sources of the bacterial contamination for cellular blood products including donor bacteremia, and contamination during whole blood collection and of the collection pack. This is followed by strategies to decrease the transfusion-associated morbidity/mortality risk of contaminated cellular blood products 576American Society of Hematology including improving donor skin disinfection, removal of first aliquot of donor blood, pretransfusion detection of bacteria, reducing recipient exposure, and pathogen reduction/inactivation. In the final sections, Drs. Vostal, Epstein and Goodman describe the regulations and regulatory approaches critical to the appropriate implementation of a bacterial contamination screening and limitation program including their and/or the FDA's input on prevention of bacterial contamination, bacterial proliferation, and detection of bacteria in transfusion products. This is followed by a discussion of sampling strategy for detection of bacteria in a transfusion product, as well as the current approval process for bacterial detection devices, trials recommended under "actual clinical use" conditions, pathogen reduction ...

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“…Transfusion associated sepsis cases are encountered occasionally and bacterial transmission remains the major cause of acute morbidity and mortality following post transfusion infection [1][2][3][4]. Bacterial contamination of platelet products is generally the most frequent infectious risk associated with transfusion [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…Possible mechanisms of bacterial contamination of blood components include donor bacteremia, contamination during the whole blood collection procedure, contamination of the collection pack or contamination during the blood processing procedure [3,4,9]. The types of organisms isolated in these transmissions indicate that the major source of contamination is skin derived [1]. Micro organisms are present on skin of all individuals but are numerous only in relatively moist areas [9].…”

Section: Introductionmentioning

confidence: 99%

Impact of Donor Arm Cleaning with Different Aseptic Solutions for Prevention of Contamination in Blood Bags

Patel

Shukla

Gupte

2012

Indian J Hematol Blood Transfus

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Transfusion associated sepsis cases are encountered occasionally and bacterial transmission remains the major cause. The goal of our study was to compare the efficacy of disinfectants in phlebotomy site preparation. After selection of donor the antecubital fossa area of the arm was disinfected with different types of disinfectants namely sprit (70% isopropyl alcohol), povidone iodine (0.5% w/v available iodine in distilled water), savlon (1.5% v/v chlorhexidine gluconate solution and 3.0% cetrimide solution) and combination of sprit and povidone iodine. Swabs were collected from 20 donors using a sterile forceps, after cleaning with different antiseptic solutions. Swab was streaked on blood agar plate aseptically and the plate was incubated at 37°C for 24 h. Colonies were counted and a single colony was recultured by growing on nutrient and Mac-Conkey agar. The biochemical characteristics were determined by performing Gram staining, Motility, Catalase and Oxidase tests. The mean values of colonies were significantly higher with savlon compared to other three solutions. The difference was statistically significant by ''t'' test (t values 1.7-3.0; P \ 0.05). Staphylococcus epidermidis, Staphylococcus sp., Streptococcus sp., Micrococcus sp., Bacillus megaterium and Bacillus cereus were the organisms identified. After completion of bleeding, samples from the bag were aseptically inoculated in aerobic and anaerobic culture bottles to be tested on BacT/Alert system. The bag containing donor's blood did not show any contamination when three cleanings were carried out using sprit, povidone iodine and spirit respectively.

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Evaluation of donor arm disinfection techniques

Abstract: The Medi-Flex disinfection method offers the English National Blood Service a validated, optimal 'best practice' disinfection technique and should contribute significantly to the reduction in risk of transmission of bacteria by transfusion.

Cited by 88 publications

References 9 publications

Fixation of the short-term central venous catheter. A comparison of two techniques

Fixation of the short-term central venous catheter. A comparison of two techniques

Bacterial Contamination of Blood Components: Risks, Strategies, and Regulation

Impact of Donor Arm Cleaning with Different Aseptic Solutions for Prevention of Contamination in Blood Bags

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