Evaluation of dried blood spots as an alternative sampling strategy for 5-fluorouracil monitoring: From method development to clinical application

Silva, Laura Cé da; Grando, Ana Paula; Baco, Lucas Silva de; Hahn, Roberta Zilles; Filho, A. F. Ferreira; Brucker, Natália; Linden, Rafael; Antunes, Marina Venzon

doi:10.1016/j.jpba.2023.115539

Cited by 3 publications

(1 citation statement)

References 30 publications

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“… 1 Furthermore, since TDM requires repeated sampling in short intervals, BµS are of great interest. In the last 2 years, BµS has been studied in the monitoring of immunosuppressant drugs used after organ transplantation like tacrolimus and the associate biomarker creatinine, 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 anti‐cancer such as PARP and kinase inhibitors, 55 , 56 , 57 , 58 antibiotics in children and neonates, 59 , 60 , 61 anti‐tuberculosis drugs 62 and anti‐fungal medication. 63 …”

Section: Targeted Metabolomicsmentioning

confidence: 99%

Targeted and untargeted metabolomics and lipidomics in dried blood microsampling: Recent applications and perspectives

Couacault,

Avella,

Londoño‐Osorio

et al. 2024

Analytical Science Advances

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Blood microsampling (BµS) offers an alternative to conventional methods that use plasma or serum for profiling human health, being minimally invasive and cost effective, especially beneficial for vulnerable populations. We present a non‐systematic review that offers a synopsis of the analytical methods, applications and perspectives related to dry blood microsampling in targeted and untargeted metabolomics and lipidomics research in the years 2022 and 2023. BµS shows potential in neonatal and paediatric studies, therapeutic drug monitoring, metabolite screening, biomarker research, sports supervision, clinical disorders studies and forensic toxicology. Notably, dried blood spots and volumetric absorptive microsampling options have been more extensively studied than other volumetric technologies. Therefore, we suggest that a further investigation and application of the volumetric technologies will contribute to the use of BµS as an alternative to conventional methods. Conversely, we support the idea that harmonisation of the analytical methods when using BµS would have a positive impact on its implementation.

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Section: Targeted Metabolomicsmentioning

confidence: 99%

Targeted and untargeted metabolomics and lipidomics in dried blood microsampling: Recent applications and perspectives

Couacault,

Avella,

Londoño‐Osorio

et al. 2024

Analytical Science Advances

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Alginate‐derived carbon dots for “turn off–on” anti‐neoplastic 5‐fluorouracil sensing in biological samples

Majd,

Kashanian,

Babaei

et al. 2024

Biotech and App Biochem

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As a chemotherapy drug, 5‐fluorouracil (5‐FU) has been used for colon cancer for decades. Excessive levels of 5‐FU in the human body can lead to notable adverse effects, including severe diarrhea, infection, mouth sores, skin peeling, skin inflammation, and ulcers, which are important and relatively common digestive side effects. In addition, 5‐FU is an analog of uracil and also has similarities to pyrimidines. Therefore, it is not easy to separate them. This research presented a sensor capable of detecting drugs in minimal amounts. An alginate‐derived carbon dot (CD) was synthesized by unique optical properties that obey an on–off fluorescence mechanism for 5‐FU sensing. Introducing copper (Cu(I)) to CDs results in fluorescence quenching through electron transfer. However, when 5‐FU is added to the system as an oxidizing agent, a redox reaction occurs on the surface of the CDs, which leads to the restoration of fluorescence as Cu(I) is altered to Cu(II). Experimental results showed a strong linear correlation (R2 = 0.99) in the concentration range of 1.00–45.00 nM, with the following linear regression, and revealed the relative standard deviation (RSD%) and detection limit of 2.57%, and 1.00 nM, respectively. These results validated the excellent detection capability of the proposed method even at low concentrations of 5‐FU and in the presence of other drugs and interfering substances. Also, the recovery of 5‐FU (varies from 100.46% to 113.7%, with RSD equal to 1.89–3.63) in serum samples indicates the absence of matrix interference in the determination of 5‐FU. In summary, this novel approach to developing a cost‐effective and sensitive sensor holds great potential for future applications in healthcare and related fields.

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Smartcard: an integrated approach for contaminant monitoring, from field to laboratory

Geballa-Koukoula,

Willemsen,

Beij

et al. 2024

Anal Bioanal Chem

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Effective food safety monitoring requires a multi-step approach from farm to fork, involving different methods, ranging from convenient screening devices to sophisticated laboratory confirmatory testing. However, sample transportation to routine laboratories is time-consuming and expensive. Simplified on-site sampling followed by laboratory analysis offers a potential solution. Dried blood spot (DBS) cards ensure stability and ease of sample transportation and are used in clinical testing. However, the applicability of such an approach could be broader and include the storage of dried extract from more complex (solid) matrices. Therefore, a simplified approach is presented here, using DBS cards for on-site sampling and subsequent laboratory confirmation for food contaminants. To achieve this, an analytical tool (Smartcard) was designed using 3D-printing technology. As a proof of concept, the approach was applied to detect the pesticide fipronil, which is widely used in ornamental flower production to limit pests and on poultry farms. The Smartcard can securely store the sample extracts on a DBS card (dried extract spot (DExS) card), incorporate the lateral flow immunoassay (LFIA) and immediately provide an estimate of contamination levels. After simplified in-syringe extraction of the sample, the LFIA allows direct screening of fipronil (half maximum inhibitory concentration of 6.5 µg/l with calibration standards), and the same sample extract can be directly applied to the DExS card for storage and transport to the laboratory, where analyte re-extraction and instrumental analysis is performed using ultra high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detecting fipronil down to 0.8 µg/kg. Graphical Abstract

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Evaluation of dried blood spots as an alternative sampling strategy for 5-fluorouracil monitoring: From method development to clinical application

Cited by 3 publications

References 30 publications

Targeted and untargeted metabolomics and lipidomics in dried blood microsampling: Recent applications and perspectives

Targeted and untargeted metabolomics and lipidomics in dried blood microsampling: Recent applications and perspectives

Alginate‐derived carbon dots for “turn off–on” anti‐neoplastic 5‐fluorouracil sensing in biological samples

Smartcard: an integrated approach for contaminant monitoring, from field to laboratory

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