Evaluation of dry stored disposable sensor strip on rapid SARS-CoV-2 detection platform

Abstract

This study investigated a SARS-CoV-2 virus detection mechanism using dry-stored disposable strips. The accuracy of this sensing platform is as good as polymerase chain reaction (PCR) with a detection time of fewer than 30 s. SARS-CoV-2 antibodies were biofunctionalized on disposable strips similar to glucose detection strips to detect the presence and concentrations of SARS-CoV-2 in saliva samples. Eight 1 ms electric pulses were sent through the sensor strip with a saliva sample in its microfluidic chamber. A circuit board embedded with MOSFET was also employed to amplify the detected signals and convert the signal to digital readings displayed on an LCD screen. The COV-antibody functionalized disposable strips were stored in a dry condition for at least one day before analyzing clinical human saliva samples with known cycling threshold (Ct) values confirmed with conventional PCR tests. Results demonstrate our system is capable of showing qualitative positive or negative results within 30 s and providing quantitative SARS-CoV-2 concentrations in terms of Ct values in 5 min.

Point-of-Care Detection of HER2 and CA 15-3 in Breast Cancer Patients: Dual-Channel Biosensor Implementation

“…The details of the functionalization method have been previously elucidated. 26,[38][39][40] A solution of 10 mM thioglycolic acid (TGA) was employed to establish a robust Au-S bond, with N, N'-Dicyclohexylcarbodiimide (DCC) and N-Hydroxysuccinimide (NHS) aiding in the formation of an amide bond. This amide bond is crucial for interaction with accessible amino groups on an antibody.…”