“…After 14 days, the sponge was gently removed, and the serum gonadotropin of a pregnant mare was injected intramuscularly (PMSG; 500 IU per animal; Folligon, Intervet Int., Boxmeer, Netherlands) to facilitate ovulation. Oestrus occurred within 48-72 h after PMSG administration (Krawczyńska et al, 2019;Rekešiutė et al, 2020). The appearance of oestrus was additionally detected using a vasectomised ram.…”