Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population

Greenland-Bews, Caitlin; Byrne, Rachel L; Owen, Sophie I; Watkins, Rachel L; Bengey, Daisy; Buist, Kate; Clerkin, Karina; Escadafal, Camille; Finch, Lorna; Gould, Susan; Giorgi, Emanuele; Hodgkinson, Andy; Mashenko, Larysa; Powell, Darren; Savage, Helen R; Thompson, Caitlin R.; Turtle, Lance; Wardale, Jahanara; Wooding, Dominic; Edwards, Thomas; Atienzar, Ana I Cubas; Adams, Emily

doi:10.1186/s12879-023-08033-1

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…When utilized related to symptomatology, a determination of contamination might be conceivable. Fast antigen test using anti-CoV antibodies rather than immobilized viral antigen, allowing for a more easy assessment of the current disease [ 151 ].…”

Section: Computational Framework For Covid-19 Diagnostics and Therape...mentioning

confidence: 99%

Reviewing methods of deep learning for diagnosing COVID-19, its variants and synergistic medicine combinations

Rafique,

Rehman,

Afghan

et al. 2023

Computers in Biology and Medicine

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Section: Computational Framework For Covid-19 Diagnostics and Therape...mentioning

confidence: 99%

Reviewing methods of deep learning for diagnosing COVID-19, its variants and synergistic medicine combinations

Rafique,

Rehman,

Afghan

et al. 2023

Computers in Biology and Medicine

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Section: Lfia and Response To Vaccinationmentioning

confidence: 99%

“…A very recent study assessed the sensitivity of eight LFIAs in post-vaccinated individuals and found that the sensitivity increased from dose one to dose two in six out of eight LFIAs [127] . Three tests detecting anti-spike antibodies achieved 100% sensitivity at dose two, compared with a chemiluminescent microparticle immunoassay [127] . Another recent study showed that a LFIA (Humasis®, Anyang, South Korea) was able to detect anti-spike antibodies in plasma samples of vaccinated subjects with an accuracy comparable to ELISA, and the strength of label bands correlated with antibody concentrations, although effective protection against infection was not assessed [128] .…”

Section: Lfia and Response To Vaccinationmentioning

confidence: 99%

An update on lateral flow immunoassay for the rapid detection of SARS-CoV-2 antibodies

Spicuzza¹,

Campagna²,

Maria³

et al. 2023

AIMSMICRO

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<abstract> <p>Over the last three years, after the outbreak of the COVID-19 pandemic, an unprecedented number of novel diagnostic tests have been developed. Assays to evaluate the immune response to SARS-CoV-2 have been widely considered as part of the control strategy. The lateral flow immunoassay (LFIA), to detect both IgM and IgG against SARS-CoV-2, has been widely studied as a point-of-care (POC) test. Compared to laboratory tests, LFIAs are faster, cheaper and user-friendly, thus available also in areas with low economic resources. Soon after the onset of the pandemic, numerous kits for rapid antibody detection were put on the market with an emergency use authorization. However, since then, scientists have tried to better define the accuracy of these tests and their usefulness in different contexts. In fact, while during the first phase of the pandemic LFIAs for antibody detection were auxiliary to molecular tests for the diagnosis of COVID-19, successively these tests became a tool of seroprevalence surveillance to address infection control policies. When in 2021 a massive vaccination campaign was implemented worldwide, the interest in LFIA reemerged due to the need to establish the extent and the longevity of immunization in the vaccinated population and to establish priorities to guide health policies in low-income countries with limited access to vaccines. Here, we summarize the accuracy, the advantages and limits of LFIAs as POC tests for antibody detection, highlighting the efforts that have been made to improve this technology over the last few years.</p> </abstract>

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Studies on the ssDNA-Based Biosensor Regeneration and Miniaturization for Electrochemical Detection of miRNAs

Zaras,

Sokal,

Jarczewska

2024

J. Electrochem. Soc.

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One of the most significant disadvantages of biosensing systems is the limited possibility of their regeneration, which only allows for their single use for detection of most targets. The reduction of biosensor fabrication cost could thus be achieved by elaboration of protocol providing the highest recovery of sensing layer response. A further drop of production expenses could yield the biosensor miniaturization as it leads to consumption of chemicals required for receptor layer formation as well as execution of measurements. To address the above-mentioned challenges, we aimed to find the most adequate method of regeneration of single-stranded DNA-based layers specific to miRNA 141 molecule which elevated concentration might refer to progression of cancer. The studies indicated that 5 min. incubation of ssDNA-modified electrode in 4 M urea provided the highest response towards miRNA 141 among all tested regeneration procedures. Furthermore, the possibility of ssDNA immobilization on was shown. This enabled miRNA 141 detection within 0.1 nM – 1 µM concentration range with high selectivity. Moreover, ssDNA layers elaborated on miniaturized transducers were distinguished with sufficient stability after 24 h storage in 20 mM PBS and could be also regenerated using 4 M urea.

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Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population

Cited by 3 publications

References 17 publications

Reviewing methods of deep learning for diagnosing COVID-19, its variants and synergistic medicine combinations

Reviewing methods of deep learning for diagnosing COVID-19, its variants and synergistic medicine combinations

An update on lateral flow immunoassay for the rapid detection of SARS-CoV-2 antibodies

Studies on the ssDNA-Based Biosensor Regeneration and Miniaturization for Electrochemical Detection of miRNAs

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