particulate matter (particle size > 10 µm), inhalable particulate matter (particle size < 10 µm), and fine particulate matter (PM 2.5 , equivalent diameter < 2.5 µm). [1] PM 2.5 particles are particularly harmful because they can be inhaled into the lungs and even enter the blood circulatory system, causing health issues. [2] PM 2.5 particles are also a good carrier of bacteria and viruses, resulting in the transmission of diseases, such as COVID-19. [3] Filtration with a filter is an effective method to remove these harmful particles and prevent the diseases from spreading.The removal mechanism of fibrous filters for these tiny particles mainly includes inertial collision, [4,5] direct interception, [6] diffusion effect, [7] gravity sedimentation, [8] and electrostatic attraction. [9] Nanofibrous filters have outstanding filtration efficiency due to the fine fiber diameters and high specific surface areas. [10] In addition, nanofibers have lower pressure drop due to the "slip effect" of their unique fiber diameter size, similar to the mean air molecule free path, reducing the airflow resistance. [11] Hence, nanofibers are widely used in air filtration, especially in air filters, [12][13][14] ventilating and air conditioning filters, [15] and masks. [16] Xia et al. [17] compared 122 nanofibrous filters with conventional heating, ventilation, and air-conditioning (HVAC) filters and showed that nanofiber filters had a higher quality factor than the conventional filters because of nanofibers' "slip effect."Considerable research has been devoted to understanding the structural parameters of nanofibers and their effect on filtration performance. For example, Wang et al. [18] studied the influence of nanofiber morphology on filtration performance. Zhang et al. [19] reported a pore size gradient combination of different nanofibrous membranes, which showed a filtration efficiency of 99.992% for 300 nm particles at a low-pressure drop. Li et al. [20] proposed a mathematical relationship between filtration efficiency and nanofibrous pore size. Bian et al. [21] established a semiempirical model to predict the influence of nanofiber diameter, membrane thickness, and packing density on the pressure drop of nanofibrous filters. Most studies have been based on nanofiber membranes with a homogenous distribution of nanofibers along the membrane.This study reports a patterned nanofibrous membrane and its novel filtration performance. The nanofibers are prepared using an electrospinning technique, and the nanofibers are collected on a commercial nonwoven fabric covering a roller collector, which is prepared using a metal sheet with a round hole array. The nanofibers collected on the metal surface have a much denser structure than those in the hole areas. As a result, the patterned nanofibrous membrane has a different filtration performance (lower pressure drop and filtration efficiency) from the uniform nanofiber membrane, prepared under the same condition but using a smooth roller collector. When two layers of this patterned nanof...