Evaluation of encapsulated anethole and carvone in lambs artificially- and naturally-infected with Haemonchus contortus

Katiki, Luciana Morita; Araújo, Renata Cristina; Ziegelmeyer, L.; Gomes, A. C. P.; Gutmanis, Gunta; Rodrigues, Luan Fernandes; Bueno, Mauro Sartori; Verı́ssimo, Cecı́lia José; Louvandini, Hélder; Ferreira, Jorge; Amarante, Alessandro Francisco Talamini do

doi:10.1016/j.exppara.2019.01.002

Cited by 31 publications

(22 citation statements)

References 20 publications

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“…Parasitic gastroenteritis has been increasingly recognized as a limiting factor for sustainable small ruminant farming globally due to the adverse impact on the health and productivity of the affected animals [ 1 ]. The efficacy of broad-spectrum anthelmintics, the mainstay tool used in helminth treatment and control plans, has been compromised by the multi-drug-resistant parasite populations [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro

et al. 2020

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The anthelmintic effects of extracted coriander oil and five pure essential oil constituents (geraniol, geranyl acetate, eugenol, methyl iso-eugenol, and linalool) were tested, using larval motility assay, on the third-stage larvae (L3s) of Haemonchus contortus, Trichostrongylus axei, Teladorsagia circumcincta, Trichostrongylus colubriformis, Trichostrongylus vitrinus and Cooperia oncophora. Coriander oil and linalool, a major component of tested coriander oil, showed a strong inhibitory efficacy against all species, except C. oncophora with a half maximal inhibitory concentration (IC50) that ranged from 0.56 to 1.41% for the coriander oil and 0.51 to 1.76% for linalool. The coriander oil and linalool combinations conferred a synergistic anthelmintic effect (combination index [CI] <1) on larval motility comparable to positive control (20 mg/mL levamisole) within 24 h (p < 0.05), reduced IC50 values to 0.11–0.49% and induced a considerable structural damage to L3s. Results of the combined treatment were validated by quantitative fluorometric microplate-based assays using Sytox green, propidium iodide and C12-resazurin, which successfully discriminated live/dead larvae. Only Sytox green staining achieved IC50 values comparable to that of the larval motility assay. The cytotoxicity of the combined coriander oil and linalool on Madin–Darby Canine Kidney cells was evaluated using sulforhodamine-B (SRB) assay and showed no significant cytotoxic effect at concentrations < 1%. These results indicate that testing essential oils and their main components may help to find new potential anthelmintic compounds, while at the same time reducing the reliance on synthetic anthelmintics.

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Section: Introductionmentioning

confidence: 99%

Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro

et al. 2020

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“…The in vitro evaluation of the binary combination of R-CNE with other bioactive phytochemicals, such as anethole, showed synergistic activity in the egg hatch assay with H. contortus eggs (7). However, the in vivo administration of R-CNE+anethole combination to infected lambs for 45 days resulted in a reduction in fecal egg counts of H. contortus, but there was no effect on total worm count (9). Proper dosing regimen and formulation are essential to ensure the best performance after the administration of a bioactive phytochemical to ruminants.…”

Section: Discussionmentioning

confidence: 98%

“…The 50% lethal concentration of CNE was 85 µg/mL, and this effect was increased after the combination with different phytochemicals (7). Moreover, the effect of the long-term administration of encapsulated R-CNE was recently evaluated in sheep, and a significant reduction in the fecal egg count was obtained (9). These promissory observations show the need for additional research on the potential of this phytochemical compound as a pharmacological alternative to treat gastrointestinal parasitic diseases of ruminants.…”

Section: Introductionmentioning

confidence: 97%

Plant-Derived Compounds as a Tool for the Control of Gastrointestinal Nematodes: Modulation of Abamectin Pharmacological Action by Carvone

et al. 2020

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The combination of synthetic anthelmintics and bioactive phytochemicals may be a pharmacological tool for improving nematode control in livestock. Carvone (R-CNE) has shown in vitro activity against gastrointestinal nematodes; however, the anthelmintic effect of bioactive phytochemicals either alone or combined with synthetic drugs has been little explored in vivo. Here, the pharmacological interaction of abamectin (ABM) and R-CNE was assessed in vitro and in vivo. The efficacy of this combination was evaluated in lambs naturally infected with resistant gastrointestinal nematodes. Additionally, the ligand and molecular docking of both molecules to P-glycoprotein (P-gp) was studied in silico. The presence of R-CNE produced a significant (p < 0.05) increase of Rho123 and ABM accumulation in the intestinal explants. After 60 min of incubation, Rho123 incubated with R-CNE had a 67 ± 21% higher concentration (p < 0.01) than when it was incubated alone. In the case of ABM, a significant increase in the intestinal concentrations was observed at 15 and 30 min after incubation with R-CNE. In the in vivo assay, no undesirable effects were observed after the oral administration of R-CNE. The coadministration of the natural compound prolonged ABM absorption in lambs. ABM T½ absorption was 1.57-fold longer (p < 0.05) in the coadministered group. Concentrations of R-CNE between 420 and 2,593 ng/mL were detected in the bloodstream between 1 and 48 h posttreatment. The in vivo efficacy of ABM against gastrointestinal nematodes increased from 94.9 to 99.8% in the presence of R-CNE, with the lower confidence interval limit being >90%. In vitro/in vivo pharmacoparasitological studies are relevant for the knowledge of the interactions and the efficacy of bioactive natural products combined with synthetic anthelmintics. While ADMET (absorption, distribution, metabolism, excretion, and toxicity) predictions and the molecular docking study showed a good interaction between ABM and P-gp, R-CNE does not appear to modulate this efflux protein. Therefore, the pharmacokinetic–pharmacodynamic effect of R-CNE on ABM should be attributed to its effect on membrane permeability. The development of pharmacology-based information is critical for the design of successful strategies for the parasite control.

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“…El TRCFH fue utilizado en todos los trabajos incluidos en la presente revisión. (66,67,70,72), junto con la evaluación de la función renal y hepática (66,72). Adicionalmente, el seguimiento de variables productivas como la ganancia diaria de peso (GDP) y la conversión alimenticia reviste una gran importancia en términos de aplicabilidad en diferentes sistemas productivos.…”

Section: Experiencias En La Utilización De Terpenos Y Aceites Esenciales Sobre Los Nematodos Gastrointestinales De Ovinos Y Caprinosunclassified

Actividad antihelmíntica in vivo de terpenos y aceites esenciales en pequeños rumiantes

Torres-Fajardo

Higuera-Piedrahita

2021

Rev MVZ Córdoba

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Los terpenos y aceites esenciales (AcEs) poseen un amplio espectro de actividades biológicas que pueden ser exploradas en las ciencias veterinarias. En este sentido, su uso ha sido propuesto como una estrategia para enfrentar las crecientes poblaciones de nematodos gastrointestinales (NGI) resistentes a los antihelmínticos (AH) tradicionales. En la presente revisión analizamos 11 manuscritos científicos que, mediante la utilización del enfoque in vivo, evaluaron el potencial AH de plantas ricas en terpenos (PRT) o AcEs en pequeños rumiantes. La especie ovina fue utilizada en el 81% de los trabajos. Brasil es el país que lidera esta línea de investigación seguido de los Estados Unidos de América y la República de Benín. Todos los manuscritos analizados utilizaron la prueba de reducción del conteo fecal de huevos de NGI, mientras que cinco manuscritos emplearon la metodología del test —o prueba— controlado(a). La actividad de las PRT y los AcEs sobre la excreción de huevos de NGI en las heces fue variable, reportándose valores que oscilaron desde un efecto nulo hasta un 97%. Dos trabajos reportaron una reducción en el tamaño de los NGI machos adultos y en la fecundidad de las hembras tras la administración de AcEs. Resulta necesario generar más trabajos que se dirijan a entender las interacciones entre las plantas, sus metabolitos secundarios y los rumiantes que las consumen. Comprender dichas interacciones nos permitirá utilizar estos productos naturales como elementos que ayuden a mejorar la nutrición y la sanidad de ovinos y caprinos en diferentes sistemas productivos.

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Evaluation of encapsulated anethole and carvone in lambs artificially- and naturally-infected with Haemonchus contortus

Cited by 31 publications

References 20 publications

Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro

Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro

Plant-Derived Compounds as a Tool for the Control of Gastrointestinal Nematodes: Modulation of Abamectin Pharmacological Action by Carvone

Actividad antihelmíntica in vivo de terpenos y aceites esenciales en pequeños rumiantes

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