Evaluation of endoscopic examination of colon tumors in rats

Narisawa, Tomio; Wong, Ching-Quo; Weisburger, John H.

doi:10.1007/bf01070878

Cited by 28 publications

(5 citation statements)

References 9 publications

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“…The authors successfully performed TC on 37 of 39 rats with a pediatric bronchoscope. Colonoscopy limited to the splenic flexure in rats has been previously reported [5,8]. Recently, colonoscopy in mice was reported, although the mean length of insertion was 3 cm [6].…”

Section: Discussionmentioning

confidence: 94%

“…Recently, colonoscopy in mice was reported, although the mean length of insertion was 3 cm [6]. However, these studies did not address the question as to what extent TC is reproducible [5][6][7][8]. The next challenge is to use the working channel of a fiberscope to inject a cell suspension into the cecal wall of the rat.…”

Section: Discussionmentioning

confidence: 99%

“…The next challenge is to use the working channel of a fiberscope to inject a cell suspension into the cecal wall of the rat. These studies did not include endoscopic submucosal injection of the cecal wall [5][6][7][8].…”

Section: Discussionmentioning

confidence: 99%

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Development of a total colonoscopy rat model with endoscopic submucosal injection of the cecal wall

et al. 2005

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Development of a total colonoscopy rat model with endoscopic submucosal injection of the cecal wall

et al. 2005

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“…At week 11, the rats of the untreated group were submitted to endoscopic examination of the large bowel according to a method reported previously (Narisawa et al 1975;Merz et al 1981), to confirm that no grossly detectable large bowel tumors had developed up to this time. The rats were sacificed and autopsied at weeks 21 or 31.…”

Section: Methodsmentioning

confidence: 99%

Reduction of acetoxymethyl-methylnitrosamine-induced large bowel cancer in rats by indomethacin.

Narisawa

Hermanek

Schmähl

1984

Tohoku J. Exp. Med.

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“…Several studies have reported on attempts to perform colonoscopy in rats (1)(2)(3)(4)(5)(6)(7)(8)(9). However, in these studies, the rats were either large and old, the bowel was not optimally prepared, a total colonoscopy was not performed or no biopsies were taken.…”

Section: Introductionmentioning

confidence: 99%

Colonoscopy with mucosal biopsies in young rats: A model for experimental gastroenterology

Wendelbo¹,

Gundersen²,

Hatlebakk³

et al. 2013

Molecular Medicine Reports

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Abstract.Endoscopy is an important tool in the diagnosis, follow-up and management of several gastrointestinal diseases. In experimental studies, the course and progression of gastrointestinal lesions are followed by sacrificing animals at specific time intervals and the disease is usually assessed postmortem. This approach has several disadvantages, including the requirement of a large number of experimental animals and the inability to evaluate and follow the progression of the same lesion. The present study tested the feasibility of performing a total colonoscopy with mucosal biopsies in small, young rats. Eighteen adult male Wistar rats with an average bodyweight of 194 g (range, 166-232 g) were used. Three bowel preparation regimes with different fasting times and doses of sodiumpicosulfate and magnesium citrate were examined. A video gastroscope with a 4.9 mm outer diameter, 210/120˚ up/down tip deflection, 103 cm working length, 140˚ view field and a 2 mm working channel (GIF-N180, Olympus, Tokyo, Japan) was used. The rats were anesthetized by inhalation of isoflurane prior to and during colonoscopy. Biopsies were obtained using biopsy forceps through the endoscope working channel. The animals were allowed to recover and were monitored for ~1 h, following the procedure. Sections from the biopsy samples taken during colonoscopy and colon tissue obtained during postmortem laparotomy were stained with hematoxylin and eosin. The optimum results were obtained with the bowel preparation that involved rats fasting for 24 h and the administration of 1 and 2 ml of sodium-picosulfate/magnesium citrate at 24 and 12 h prior to colonoscopy, respectively. Colonoscopy was performed with minor complications, with the only difficulty arising when maneuvering the endoscope past the hepatic flexure. This was achieved by manual rotation of the endoscope shift to the right and left of a two-way endoscope.Taking biopsies with the forceps at full jaw length and swing resulted in perforation, which may be avoided by obtaining biopsies with the outer tip of the biopsy forceps. IntroductionEndoscopy is an important tool in the diagnosis, follow-up and management of several gastrointestinal diseases, including inflammatory bowel disease and cancer. The endoscopic features and results of biopsy examinations are the parameters used for evaluation in clinical practice. In experimental studies, the course and progression of gastrointestinal lesions are followed by sacrificing animals at specific time intervals. The disease is usually assessed postmortem. This approach has several disadvantages: i) it requires a large number of experimental animals; ii) it is not possible to follow the progression and evaluation of the same lesion and iii) the findings are difficult to compare with biopsy samples obtained from patients by endoscopy.Several studies have reported on attempts to perform colonoscopy in rats (1-9). However, in these studies, the rats were either large and old, the bowel was not optimally prepared, a total colonoscopy was not...

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Evaluation of endoscopic examination of colon tumors in rats

Cited by 28 publications

References 9 publications

Development of a total colonoscopy rat model with endoscopic submucosal injection of the cecal wall

Development of a total colonoscopy rat model with endoscopic submucosal injection of the cecal wall

Reduction of acetoxymethyl-methylnitrosamine-induced large bowel cancer in rats by indomethacin.

Colonoscopy with mucosal biopsies in young rats: A model for experimental gastroenterology

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