Evaluation of Entrapment Efficiency of Glipizide Microsphere

Arora, Neha

doi:10.9790/3013-0220180181

Cited by 4 publications

(2 citation statements)

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“…The supernatant was passed through a 0.2-μm syringe filter. Then 100 μL of the supernatant was distributed in 900 μL of acetonitrile, and unentrapped drug absorbance was observed through HPLC at 210 nm with above-set parameters ( Dora et al, 2010 ; Arora, 2012 ). The entrapment efficiency of SLNP was calculated as follows:…”

Section: Methodsmentioning

confidence: 99%

Fabrication and Assessment of Diosgenin Encapsulated Stearic Acid Solid Lipid Nanoparticles for Its Anticancer and Antidepressant Effects Using in vitro and in vivo Models

et al. 2022

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Inflammatory cascade plays a pivotal role in the onset and progression of major depressive disorder (MDD) and glioblastoma multiforme (GBM). Therefore, questing natural compounds with anti-inflammatory activity such as diosgenin can act as a double-edged sword targeting cancer and cancer-induced inflammation simultaneously. The blood–brain barrier limits the therapeutic efficiency of the drugs against intracranial pathologies including depression and brain cancers. Encapsulating a drug molecule in lipid nanoparticles can overcome this obstacle. The current study has thus investigated the anticancer and antidepressant effect of Tween 80 (P80) coated stearic acid solid lipid nanoparticles (SLNPs) encapsulating the diosgenin. Physio-chemical characterizations of SLNPs were performed to assess their stability, monodispersity, and entrapment efficiency. In vitro cytotoxic analysis of naked and drug encapsulated SLNPs on U-87 cell line indicated diosgenin IC50 value to be 194.4 μM, while diosgenin encapsulation in nanoparticles slightly decreases the toxicity. Antidepressant effects of encapsulated and non-encapsulated diosgenin were comprehensively evaluated in the concanavalin-A–induced sickness behavior mouse model. Behavior test results indicate that diosgenin and diosgenin encapsulated nanoparticles significantly alleviated anxiety-like and depressive behavior. Diosgenin incorporated SLNPs also improved grooming behavior and social interaction as well as showed normal levels of neutrophils and leukocytes with no toxicity indication. In conclusion, diosgenin and diosgenin encapsulated solid lipid nanoparticles proved successful in decreasing in vitro cancer cell proliferation and improving sickness behavioral phenotype and thus merit further exploration.

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Section: Methodsmentioning

confidence: 99%

Fabrication and Assessment of Diosgenin Encapsulated Stearic Acid Solid Lipid Nanoparticles for Its Anticancer and Antidepressant Effects Using in vitro and in vivo Models

et al. 2022

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“…6,7,8 Materials: Diltiazem hydrochloride was a gift sample from Hetero pharma, Hyderabad. Sodium alginate, HPMC K15M, carbopol 934, calcium chloride are from LOBA CHEME and all the other ingredients used in the study were analytical grade.…”

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confidence: 99%

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2017

IJPSR

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Diltiazem hydrochloride is a calcium channel blocker used in various cardiac diseases. It is associated with problem of short biological half life leading to high dosage frequency. Therefore, to ensure the controlled drug delivery systems of diltiazem the alginate-chitosan microspheres were prepared by Ionotropic gelation technique and characterized. The diltiazem hydrochloride SR microspheres were successfully formulated by Ionotropic gelation technique employing different proportions of sodium alginate, hydroxy propyl methyl cellulose (HPMC) K15M and carbopol 934 in various combinations. The prepared diltiazem hydrochloride microspheres were characterized for entrapment efficiency, swelling index, Carr's index, angle of repose, particle size, assay and in-vitro drug release. Further, the final optimized formulations were assessed for their compatibility studies using Fourier Transform Infrared (FT-IR) Spectroscopy and stability was assessed by conducting accelerated stability studies. All the diltiazem hydrochloride microsphere formulations were found have good flow properties. The mean particle size was found to be within a range of 6.1-to 9.2 μm and entrapment efficiency ranged from 70% to 94.6%. In-vitro drug release was found to be 96% for the optimized formulation. FTIR studies were carried out and all the excipients are found to be compatible with no interactions. Stability studies were carried out for the best formulation F5 indicates that there is no change in entrapment efficiency and release kinetics. The results obtained in this work clearly indicate best results using carbopol 934 a rate controlling polymer in diltiazem hydrochloride SR microspheres.

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