Evaluation of Enzyme Protease Activity and Inhibition Effect on Pyricularia grisea with the Leaf Extract of Commela communis L.

Abstract

Rice blast is a dangerous disease that causes major damage to rice productivity worldwide. The purpose of this report is to examine the protease activity of Pyricularia grisea and the effects of Commelina communis L. (common name "day flower") extract on mycelial growth. The result showed that three isolates were collected from wild rice (Oryza rufipogon) based on a designation of new international standard and namely as U43-i4-k024-z05-ta532 (isolate 1); U12-i0-k101-z05-ta102 (isolate 2) and U43-i4-k141-z15-ta522 (isolate 3). Particularly, the third isolate showed the highest protease enzyme activity of 30.11 U/ml, followed by isolate 1 (20.27 U/ml) and isolate 2 (12.81 U/ml). The new fungi isolated from wild rice may be important methods to control detrimental diseases infesting cultivated rice. The result showed methanol extract from the day flower inhibitions on P.grisea. Besides protease activity, fungi inhibition was also affected. The protease activity was low; the mycelial growth inhibition was high (IC 50 = 2.35mg/mL at Isolate 2). Plant extracts from the day flower saved money and were environmentally friendly. So the implementation of this substance is very essential.

The effect of chemical-induced mutagenesis in indica rice variety IR50404 for blast disease caused by the Magnaporthe grisea in Vietnam

“…The blast fungus was isolated from wild rice by Truc et al (2019) and used for the screening of the mutant lines to analyze the disease reaction. IR 50404 seed stock was surface sterilized by soaking it in 0.1% NaOCl for 30 minutes and then washing it in distilled water.…”

“…Rice blast disease, caused by the fungus Pyricularia grisea Sacc [syn: Magnaporthe grisea (Hebert) Barr], is one of the most damaging fungi to rice production (Truc et al 2019), with up to 50% yield losses (Yang at al. 2024).…”