Evaluation of epigallocatechin-3-gallate (EGCG) modified collagen in guided bone regeneration (GBR) surgery and modulation of macrophage phenotype

Chu, Chenyu; Wang, Yufei; Wang, Zhengliang; Yang, Renli; Liu, Li; Rung, Shengan; Xiang, Lin; Wu, Yingying; Du, Shufang; Man, Yi; Qu, Yang

doi:10.1016/j.msec.2019.01.083

Cited by 78 publications

(62 citation statements)

References 36 publications

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“…Accordingly, macrophage density and inflammatory cytokines (TNF‐α, IL‐1β, IL‐6, and RANKL) were higher comparing with other Ti particles injected group without the depletion, also showed the important role of macrophage in bone growth and inflammation (Wang, Li, Feng, Cheng, & Li, ). Additionally, molecules such as inflammatory cytokines, bone morphogenetic protein 2 (BMP‐2), PDGF‐BB, and VEGF (Chu et al, ; Ng, Spiller, Bernhard, & Vunjak‐Novakovic, ), secreted by macrophage, are of great importance for bone regeneration.…”

Section: Fbr Of Implanted Materialsmentioning

confidence: 99%

Modulation of foreign body reaction and macrophage phenotypes concerning microenvironment

Chu

Liu

Rung

et al. 2019

J Biomedical Materials Res

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106

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The foreign body reaction (FBR) is described as a local chronic inflammation after implantation of biomaterials in which macrophages involved intimately. At the stage of acute inflammation, mast cells release histamine, Interleukin-4 (IL-4) andInterleukin-13 (IL-13), enhancing recruitment, and fusion of macrophages in the following phase. As for chronic intensive inflammation, degradation of biomaterials would be promoted by macrophage-derived foreign body giant cells releasing degradative enzymes, acid and reactive oxygen intermediates. Nevertheless, it could be seen as a breakthrough point for regulating FBR, considering the dominant role of the macrophage in the immune response as exemplified by the decrease of IL-4 and IL-13, stabilizing an appropriate balance between two macrophage phenotypes, selectively suppressing some function of macrophages, and so on. Moreover, the relationship between macrophages polarization and the development of a fibrous capsule, which increase the possibility of implantation failure, will be illustrated later.This review aims at providing readers a comprehensive understanding of FBR and its correlative treatment strategy. K E Y W O R D Sbiomaterials, foreign body reaction, macrophages

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Section: Fbr Of Implanted Materialsmentioning

confidence: 99%

Modulation of foreign body reaction and macrophage phenotypes concerning microenvironment

Chu

Liu

Rung

et al. 2019

J Biomedical Materials Res

Self Cite

106

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“…However, recent research found that macrophages play a critical role during bone regeneration based on their polarization into both proinflammatory and anti-inflammatory phenotypes. Immune-responsive collagen membranes may be an innovative strategy for improving guided bone regeneration surgery [36,37].…”

Section: Biomed Research Internationalmentioning

confidence: 99%

Ridge Alterations following Socket Preservation Using a Collagen Membrane in Dogs

Lyu

Shao

Zou

et al. 2020

BioMed Research International

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Background. The healing process following tooth extraction results in alveolar ridge resorption. The dimensional changes may complicate the subsequent implant procedure. Socket preservation using absorbable collagen membranes or a combination of membranes with calcium phosphate cement (CPC) particles might ensure that the alveolar ridge retains a suitable morphology for implant placement. Objective. To evaluate the quality and quantity of new bone regenerated after application of either collagen membranes alone covering the sockets or a combination of membranes with CPC particles added into the sockets in dogs. Materials and Methods. Six dogs were included in this study. The mandibular premolars were extracted. For each hemimandible, three premolar extraction sites were randomly assigned to one of the following treatments: a covering collagen membrane, CPC with a covering collagen membrane, and a socket left empty. Cone-beam computed tomography (CBCT) measurements, polyfluorochrome sequential labeling, and histological assessments were performed to investigate the healing ability and repair processes within a 6-month observation period. Results. Buccal bone height in the membrane group was significantly higher than that in the membrane+CPC and blank groups at 4 and 6 months after extraction. The mineral apposition rate over 2-4 months and the alizarin red-stained area in the membrane group were significantly higher than those in the other two groups. Histological analysis after 6 months of healing showed significantly higher amounts of newly formed bone in the membrane group than in the other groups. Conclusion. Extraction sites treated with collagen barrier membranes showed better protection than sites not covered with membranes. And the buccal bone wall of the socket was well preserved by collagen membrane without extra CPC materials. Socket preservation using absorbable membranes alone yielded better quality and quantity of regenerated bone inside the socket site.

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“…It has been reported that human amniotic mesenchymal stromal cells (HAMSC) showed increased secretion of BMP-2 and VEGF by promoting the macrophages to M2 polarization and the osteogenesis of hBMSC was enhanced via co-culture method in a paracrine manner of HAMSC [47]. Epigallocatechin-3-gallate (EGCG) modi ed collagen was reported to promote the transition of macrophage to M2 polarization, thus inhibiting in ammatory response and increased the secretion of BMP-2 and VEGF, which further facilitates the osteogenesis by upregulating RUNX2 and OPN and promotes the new bone formation in vivo [48].…”

Section: Discussionmentioning

confidence: 99%

MSC-derived miR-181b Overexpressing Exosomes Enhanced Osteointegration by Enhancing M2 Polarization of Macrophages via Targeting the PRKCD/AKT Pathway

Chen

et al. 2020

Preprint

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Background: Many patients suffer from implant loosening after the implantation of titanium alloy caused by immune response to the foreign bodies and this could inhibit osteogenesis, which could possibly give rise to poor osteointegration and there is currently no appropriate solution in clinical practice. Exosomes overexpressing miRNA has been proven to be a suitable candidate for solving this problem. In this study, we explored whether miR-181b could exert beneficial effect on promoting M2 macrophage polarization, thus inhibiting inflammation as well as promoting osteogenesis and elaborated the underlying mechanism in vitro. Furthermore, we aimed to find whether exosomes overexpressing miR-181b (Exo-181b) could enhance osteointegration in vivo.Methods: In vitro and in vivo studies were carried out for assessing the anti-inflammatory and pro-osteogenesis effect of miR-181b. In vitro, ELISA was applied for the detection of the inflammation factors levels including IL-6, TNF-α, as well as IL-10 and the percentage of M1 or M2 polarization was determined by flow cytometry. Also, qRT-PCR was used for the detection of the relative gene expression of the CCR7, CD206, Arg-1, iNOS, VEGF and BMP-2 genes. Western blotting was applied for detecting the protein expression of PRKCD, AKT and p-AKT. In vivo, we established air pouch model for evaluating the effect of Exo-181b on macrophage polarization and distal femoral bone defect model was established for determining the osteointegration effect of Exo-181b by MicroCT, sequential fluorescent labeling and histological analysis. Results: In vitro, we firstly verified that miR-181b significantly enhanced M2 polarization and inhibited inflammation by suppressing PRKCD and activating p-AKT. Then, in vivo, we verified that Exo-181b enhanced M2 polarization, reduced the inflammatory response and enhanced osteointegration. Conclusions: MiR-181b could suppress inflammatory response by regulating the PRKCD/AKT signaling pathway and promoting M2 polarization, which further promoting osteogenesis of hBMSC in vitro and Exo-181b could promote osteointegration in vivo.

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Evaluation of epigallocatechin-3-gallate (EGCG) modified collagen in guided bone regeneration (GBR) surgery and modulation of macrophage phenotype

Cited by 78 publications

References 36 publications

Modulation of foreign body reaction and macrophage phenotypes concerning microenvironment

Modulation of foreign body reaction and macrophage phenotypes concerning microenvironment

Ridge Alterations following Socket Preservation Using a Collagen Membrane in Dogs

MSC-derived miR-181b Overexpressing Exosomes Enhanced Osteointegration by Enhancing M2 Polarization of Macrophages via Targeting the PRKCD/AKT Pathway

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